bioimagesUnknownGul Civelekoglu-ScholeyThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200908150JCB

The microinjection of a mixture of anti-lamin-B mAbs crosslinks the lamin-B envelope into a hyperstable network that fails to disassemble and remains throughout mitosis. This<br />structure impedes spindle elongation.

Embryo expressing lamin-B-GFP injected with rhodamine tubulin. This embryo was imaged under the same conditions as that injected with antibody.

3D stack of an embryo expressing KLP61F-GFP during prometaphase phase 1

Cycle 11 timelapse of an embryo expressing gfp-tubulin (shown in red) and histone-rfp (shown in green)

Microinjection of the lamin-B tail dominant negative subfragment disrupts the nuclear lamina and causes defects in mitosis

Time lapse of an embryo expressing lamin-B-GFP injected with rhodamine tubulin.

Cycle 11 timelapse of an embryo expressing gfp-tubulin (shown in green) and histone-rfp (shown in red). 4 planes (0.5 microns) were acquired

3D stack of an embryo expressing Ncd-GFP during prometaphase phase 1

bioimagesFigure 5 - AImage 7995 (Figure 1 - B)Image 7990 (Figure 5 - A)Image 8072 (Figure 6 - B)Figure 1 - AImage 8071 (Figure 1 - A)Figure 1 - BFigure 5SupFigure 1Figure 6Image 7994 (Figure 1 - B)Image 8085 (Figure 6 - A)SupFigure 1 - NoneImage 8033 (SupFigure 1 - None)Figure 6 - AFigure 6 - BFigure 1Image 8032 (Figure 6 - B)Jonathan M. ScholeyLi TaoIngrid Brust-MascherGul Civelekoglu-ScholeyRoy WollmanfalsePrometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope2010-01-11T11:18:29Z2018-11-29T11:18:29Z2018-11-29T11:18:29ZS-JCBD-20090815010.1083/jcb.200908150