bioimagesUnknownDebaditya MukhopadhyayThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200909008JCB

Hela cells were treated with control siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins CENP-O (green) Cells were simultaneously stained for centromeres (CREST, red) and Hoechst 33342 (blue).

Hela cells were treated with anti-SENP6 siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins CENP-E (green) Cells were simultaneously stained for centromeres (CREST, red) and Hoechst 33342 (blue).

HeLa cells were treated with anti-SENP6 siRNA and double thymidine block for 48 h. Cells were fixed and stained for centromeres (CREST, red) Bub1 (green) and Hoechest 33342 (blue).

HeLa cells were treated with control siRNA and double thymidine block for 48 h. Cells were fixed and stained for centromeres (CREST, red) Bub1 (green) and Hoechest 33342 (blue).

Hela cells were treated with anti-SENP6 siRNA and synchronized by double thymidine block. Metaphase cells were fixed and stained with CREST sera (red), CenpH antibody (green) and Hoechst 33342 (blue).

Hela cells were treated with anti-SENP6 siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins CENP-O (green) Cells were simultaneously stained for centromeres (CREST, red) and Hoechst 33342 (blue).

Hela cells were treated with control siRNA and synchronized by double thymidine block. Metaphase cells were fixed and stained with CREST sera (red), CenpH antibody (green) and Hoechst 33342 (blue).

Hela cells were treated with anti-SENP6 siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins Hec1 (green) Cells were simultaneously stained for centromeres (CREST, red) and Hoechst 33342 (blue).

HeLa cells were treated with control siRNA for 36 h and then with nocodazole for further 12 hours. Cells were fixed and stained for centromeres (CREST, red), Mad2 (green) and Hoechest 33342 (blue).

HeLa cells were treated with anti-SENP6 siRNA for 36 h and then with nocodazole for further 12 hours. Cells were fixed and stained for centromeres (CREST, red), Mad2 (green) and Hoechest 33342 (blue).

Hela cells were treated with control siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins Hec1 (green). Cells were simultaneously stained for centromeres (CREST, red) and Hoechst 33342 (blue).

HeLa cells were treated with control siRNA and double thymidine block for 48 h. Cells were fixed and stained for centromeres (CREST, red) Bub1 (green) and Hoechest 33342 (blue).

Hela cells were treated with anti-SENP6 siRNA and synchronized by double thymidine block. Metaphase cells were fixed and stained with CREST sera (red), CenpI antibody (green) and Hoechst 33342 (blue).

Hela cells were treated with control siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins CENP-E (green) Cells were simultaneously stained for centromeres (CREST, red) and Hoechst 33342 (blue).

Hela cells were treated with control siRNA and synchronized by double thymidine block. Metaphase cells were fixed and stained with CREST sera (red), CenpI antibody (green) and Hoechst 33342 (blue).

bioimagesImage 11926 (Figure 4 - B)Image 10937 (Figure 2 - B)Image 11929 (Figure 4 - E)Figure 3 - AFigure 3 - BFigure 4 - AFigure 4 - BImage 10992 (Figure 3 - B)Image 10989 (Figure 2 - A)Image 10993 (Figure 3 - B)Figure 4 - EImage 10940 (Figure 2 - B)Image 10995 (Figure 3 - A)Image 10988 (Figure 2 - A)Image 10939 (Figure 2 - B)Image 11925 (Figure 4 - A)Image 10994 (Figure 3 - A)Figure 4Image 11927 (Figure 4 - B)Image 11924 (Figure 4 - A)Image 11928 (Figure 4 - E)Image 10938 (Figure 2 - B)Figure 2 - AFigure 3Figure 2Figure 2 - BDebaditya MukhopadhyayAlexei ArnaoutovMary DassofalseThe SUMO protease SENP6 is essential for inner kinetochore assembly2010-03-08T11:18:49Z2018-11-29T11:18:49Z2018-11-29T11:18:49ZS-JCBD-20090900810.1083/jcb.200909008