bioimagesUnknownMartijn S. LuijsterburgThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200909175JCB

Example of a photoconversion experiment on XP4PA cells transiently expressing low levels of XPC-mOrange. Cells were locally irradiated (5 m, 100 J/m2) and monitored in the orange channel (543 nm; non-photoconverted) and the far-red channel (633 nm; photoconverted). 30 min after local UV irradiation, the local accumulation of XPC-mOrange was photoconverted with 488 laser light. The levels of non-photoconverted XPC-mOrange increased at the local damage, while the levels of photoconverted XPC-mOrange decreased due to the rapid exchange of XPC at the damaged site.

bioimagesFigure 4Image 14737 (Figure 4 - None)Figure 4 - NoneWim VermeulenThomas HöferMartijn S. LuijsterburgMartijn J. MonéAudrey M. GourdinDaniël O. WarmerdamRoel van DrielAntonio Z. PolitiJoachim GoedhartGesa von BornstaedtfalseStochastic and reversible assembly of a multiprotein DNA repair complex ensures accurate target site recognition and efficient repair2010-05-03T11:18:57Z2018-11-29T11:18:57Z2018-11-29T11:18:57ZS-JCBD-20090917510.1083/jcb.200909175