{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Anne Chadrin"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-200910043"],"attach_to":["JCB"],"legend":["Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in pom33∆ +POM33 cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.","Confocal videomicroscopy analysis of Pom33-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 6.5s. The bleached area is indicated on the corresponding JPEG image.","Confocal videomicroscopy analysis of Ndc1-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 6.36s. The bleached area is indicated on the corresponding JPEG image.","Confocal videomicroscopy analysis of Sec61-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 6.97s. The bleached area is indicated on the corresponding JPEG image.<br /><br />","Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in wt cells.","Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in per33∆ cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.","Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in per33∆ pom33∆ cells.","Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in per33∆ pom33∆ cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.","Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in nup133∆ +NUP133 cells.<br /><br />","Confocal videomicroscopy analysis of Pom33-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 4.93s. The bleached area is indicated on the corresponding JPEG image.<br /><br />","Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in nup133∆ cells.","Confocal videomicroscopy analysis of Ndc1-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 4.98s. The bleached area is indicated on the corresponding JPEG image. <br /><br />","Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in nup133∆ +∆N-NUP133 cells.","Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in nup133∆ cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.","Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in nup133∆+∆N-NUP133 cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.","Confocal videomicroscopy analysis of Sec61-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 7.31s. The bleached area is indicated on the corresponding JPEG image.","The bleached area is indicated on an intermediate frame of the corresponding movie.<br /><br />","Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in pom33∆ +POM33 cells.","Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in per33∆ cells.","Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in pom33∆ cells.<br /><br />","Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in wt cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.","Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in nup133∆+NUP133 cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.","Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in pom33∆ cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.","Confocal videomicroscopy analysis of Per33-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 5.18s. The bleached area is indicated on the corresponding JPEG image.<br /><br />","The bleached area is indicated on an intermediate frame of the corresponding movie.","Confocal videomicroscopy analysis of Per33-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 7.57s. The bleached area is indicated on the corresponding JPEG image.<br /><br />"],"repository":["bioimages"],"figure_sub":["Image 8983 (Figure 2 - A.Sec61-GFP)","Image 8959 (Figure  8 - A.nup133∆ +NUP133)","Figure  8 - A.pom33∆ +POM33","Image 8952 (Figure  8 - A.pom33∆ +POM33)","Image 8979 (Figure 2 - A.Pom33-GFP)","Image 8976 (Figure 2 - A.Per33-GFP)","Image 8978 (Figure 2 - A.Pom33-GFP)","Image 8974 (Figure 2 - A.Per33-GFP)","Image 8955 (Figure  8 - A.per33∆ pom33∆)","Image 8970 (Figure 2 - A.Ndc1-GFP)","Image 8984 (Figure 2 - A.Sec61-GFP)","Image 8960 (Figure  8 - A.nup133∆)","Image 8975 (Figure 2 - A.Per33-GFP)","Image 8949 (Figure  8 - A.wt)","Image 8948 (Figure  8 - A.wt)","Image 8963 (Figure  8 - A.nup133∆ +∆N-NUP133)","Figure 2 - A.Sec61-GFP","Image 8972 (Figure 2 - A.Ndc1-GFP)","Figure  8 - A.per33∆","Image 8951 (Figure  8 - A.pom33∆)","Image 8971 (Figure 2 - A.Ndc1-GFP)","Figure  8 - A.pom33∆","Image 8973 (Figure 2 - A.Per33-GFP)","Image 8961 (Figure  8 - A.nup133∆)","Figure  8 - A.nup133∆ +NUP133","Figure  8 - A.nup133∆","Figure 2 - A.Pom33-GFP","Image 8957 (Figure  8 - A.per33∆)","Image 8954 (Figure  8 - A.per33∆ pom33∆)","Image 8969 (Figure 2 - A.Ndc1-GFP)","Figure  8 - A.per33∆ pom33∆","Image 8953 (Figure  8 - A.pom33∆ +POM33)","Image 8958 (Figure  8 - A.nup133∆ +NUP133)","Figure  8 - A.nup133∆ +∆N-NUP133","Figure  8 - A.wt","Figure 2 - A.Ndc1-GFP","Image 8977 (Figure 2 - A.Pom33-GFP)","Image 8962 (Figure  8 - A.nup133∆ +∆N-NUP133)","Figure 2 - A.Per33-GFP","Image 8956 (Figure  8 - A.per33∆)","Image 8980 (Figure 2 - A.Pom33-GFP)","Figure  8","Image 8981 (Figure 2 - A.Sec61-GFP)","Image 8982 (Figure 2 - A.Sec61-GFP)","Image 8950 (Figure  8 - A.pom33∆)","Figure 2"],"pubmed_authors":["Anne Chadrin","Mabel San Roman","Barbara Hess","Bérangère Lombard","Yves Barral","Xavier Gatti","Benoit Palancade","Valérie Doye","Damarys Loew"],"additional_accession":[]},"is_claimable":false,"name":"Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution","description":null,"dates":{"release":"2010-05-24T11:18:59Z","modification":"2018-11-29T11:18:59Z","creation":"2018-11-29T11:18:59Z"},"accession":"S-JCBD-200910043","cross_references":{"doi":["10.1083/jcb.200910043"]}}