bioimagesUnknownAnne ChadrinThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200910043JCB

Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in pom33∆ +POM33 cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.

Confocal videomicroscopy analysis of Pom33-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 6.5s. The bleached area is indicated on the corresponding JPEG image.

Confocal videomicroscopy analysis of Ndc1-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 6.36s. The bleached area is indicated on the corresponding JPEG image.

Confocal videomicroscopy analysis of Sec61-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 6.97s. The bleached area is indicated on the corresponding JPEG image.<br /><br />

Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in wt cells.

Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in per33∆ cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.

Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in per33∆ pom33∆ cells.

Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in per33∆ pom33∆ cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.

Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in nup133∆ +NUP133 cells.<br /><br />

Confocal videomicroscopy analysis of Pom33-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 4.93s. The bleached area is indicated on the corresponding JPEG image.<br /><br />

Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in nup133∆ cells.

Confocal videomicroscopy analysis of Ndc1-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 4.98s. The bleached area is indicated on the corresponding JPEG image. <br /><br />

Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in nup133∆ +∆N-NUP133 cells.

Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in nup133∆ cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.

Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in nup133∆+∆N-NUP133 cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.

Confocal videomicroscopy analysis of Sec61-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 7.31s. The bleached area is indicated on the corresponding JPEG image.

The bleached area is indicated on an intermediate frame of the corresponding movie.<br /><br />

Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in pom33∆ +POM33 cells.

Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in per33∆ cells.

Merge of the DIC image (purple) and the 3D reconstruction of total Nup84-GFP fluorescence (green) in pom33∆ cells.<br /><br />

Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in wt cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.

Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in nup133∆+NUP133 cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.

Confocal spinning disk microscopy analysis of Nup84-GFP fluorescence in pom33∆ cells. A representative Z-stack used for M/B ratio measurement is presented. Distance between planes is 0.8µm.

Confocal videomicroscopy analysis of Per33-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 5.18s. The bleached area is indicated on the corresponding JPEG image.<br /><br />

The bleached area is indicated on an intermediate frame of the corresponding movie.

Confocal videomicroscopy analysis of Per33-GFP dynamics upon continuous photobleaching of a small region within the cortical ER. Channel 0: GFP signal; Channel 1: DIC. Time between frames is 7.57s. The bleached area is indicated on the corresponding JPEG image.<br /><br />

bioimagesImage 8983 (Figure 2 - A.Sec61-GFP)Image 8959 (Figure 8 - A.nup133∆ +NUP133)Figure 8 - A.pom33∆ +POM33Image 8952 (Figure 8 - A.pom33∆ +POM33)Image 8979 (Figure 2 - A.Pom33-GFP)Image 8976 (Figure 2 - A.Per33-GFP)Image 8978 (Figure 2 - A.Pom33-GFP)Image 8974 (Figure 2 - A.Per33-GFP)Image 8955 (Figure 8 - A.per33∆ pom33∆)Image 8970 (Figure 2 - A.Ndc1-GFP)Image 8984 (Figure 2 - A.Sec61-GFP)Image 8960 (Figure 8 - A.nup133∆)Image 8975 (Figure 2 - A.Per33-GFP)Image 8949 (Figure 8 - A.wt)Image 8948 (Figure 8 - A.wt)Image 8963 (Figure 8 - A.nup133∆ +∆N-NUP133)Figure 2 - A.Sec61-GFPImage 8972 (Figure 2 - A.Ndc1-GFP)Figure 8 - A.per33∆Image 8951 (Figure 8 - A.pom33∆)Image 8971 (Figure 2 - A.Ndc1-GFP)Figure 8 - A.pom33∆Image 8973 (Figure 2 - A.Per33-GFP)Image 8961 (Figure 8 - A.nup133∆)Figure 8 - A.nup133∆ +NUP133Figure 8 - A.nup133∆Figure 2 - A.Pom33-GFPImage 8957 (Figure 8 - A.per33∆)Image 8954 (Figure 8 - A.per33∆ pom33∆)Image 8969 (Figure 2 - A.Ndc1-GFP)Figure 8 - A.per33∆ pom33∆Image 8953 (Figure 8 - A.pom33∆ +POM33)Image 8958 (Figure 8 - A.nup133∆ +NUP133)Figure 8 - A.nup133∆ +∆N-NUP133Figure 8 - A.wtFigure 2 - A.Ndc1-GFPImage 8977 (Figure 2 - A.Pom33-GFP)Image 8962 (Figure 8 - A.nup133∆ +∆N-NUP133)Figure 2 - A.Per33-GFPImage 8956 (Figure 8 - A.per33∆)Image 8980 (Figure 2 - A.Pom33-GFP)Figure 8Image 8981 (Figure 2 - A.Sec61-GFP)Image 8982 (Figure 2 - A.Sec61-GFP)Image 8950 (Figure 8 - A.pom33∆)Figure 2Anne ChadrinMabel San RomanBarbara HessBérangère LombardYves BarralXavier GattiBenoit PalancadeValérie DoyeDamarys LoewfalsePom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution2010-05-24T11:18:59Z2018-11-29T11:18:59Z2018-11-29T11:18:59ZS-JCBD-20091004310.1083/jcb.200910043