{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Azusa Hotta"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-200910095"],"attach_to":["JCB"],"legend":["MCF-10Aeco cells expressing EB1-GFP and RFP-LL5α were cocultured with parental non-fluorescent cells on glass-bottomed dishes (fluorescent:non-fluorescent cells=1:10). Z-scanning images from the apical (bottom of the Z-axis here) to basal planes (top of the Z axis) of the cell were collected using a Revolution XD system.","MCF-10Aeco cells expressing GFP-α-tubulin and RFP-LL5α were seeded on glass-bottomed dishes at a low density and cultured overnight. Time-lapse images of the cells were collected at 2.5-min intervals for 8 h 6 min using a DeltaVision Core system. During the time-lapse imaging, the cells divided into two daughter cells. The RFP-LL5α patches disappear when the cells become detached from the glass at the onset of cell division, and reappear soon after the cells adhere to the glass. Pixel Size: z=0.127, y=0.127 m; Time intervals: 2.5 min.","Mouse mammary tissues were immunostained for b-catenin (Cy2, 488 nm laser), LL5α (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.09, y=0.09 um.","MCF-10A cells were fixed and stained for LL5s (Cy5, 633 nm laser), integrin a3 (rhodamine red-X, 543 nm laser) and integrin a6 (Cy2, 488 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.04, y=0.04 um.","Mouse small intestine were immunostained for laminin (Cy2, 488 nm laser), LL5a (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.45, y=0.45 um.","MCF-10A cells were fixed and immunostained for LL5b (Cy5, 633 nm laser), laminin-5 (rhodamine red-X, 543 nm laser) and integrin a6 (Cy2, 488 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.07, y=0.07 um.","MCF-10A cells were fixed and stained for LL5s (Cy5, 633 nm laser), laminin-5 (Cy2, 488 nm laser) and integrin a3 (rhodamine red-X, 543 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.04, y=0.04 um.","At 3 h after seeding on coverslips, MCF-10Aeco cells were fixed and immunostained for tubulin (Cy2, 488 nm laser), EB1 (rhodamine red-X, 543 nm laser) and LL5s (Cy5, 633 nm laser), and observed using a LSM510 confocal laser-scanning microscope system.","Mouse mammary tissues were immunostained for laminin (Cy2, 488 nm laser), LL5a (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.09, y=0.09 um."],"repository":["bioimages"],"figure_sub":["Figure 3 - Dc","Figure 3 - Db","Image 16728 (Figure 3 - Db)","Figure 3 - Da","Figure 8 - C","Image 16799 (Figure 1 - Dc)","Figuer 4 - Ba","Image 16765 (Figuer 4 - Bb)","Figuer 4 - Bb","Video 4 - None","Image 16710 (Figure 3 - Da)","Figure 1 - Dc","Figuer 4 - D","Figure 8","Figuer 4","Image 16772 (Figuer 4 - D)","Image 16751 (Figuer 4 - Ba)","Image 16735 (Figure 3 - Dc)","Video 4","Figure 1","Image 17585 (Figure 8 - C)","Image 16705 (Video 4 - None)","Figure 3"],"pubmed_authors":["Tomomi Kawakatsu","Tomoya Nakatani","Tomoko Hamaji","Taiko Sukezane","Azusa Hotta","Chiyuki Matsui","Anna Akhmanova","Toshitaka Sato","Yuko Mimori-Kiyosue","Tsuyoshi Akagi","Yoshimi Takai","Ilya Grigoriev"],"additional_accession":[]},"is_claimable":false,"name":"Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5α/β","description":null,"dates":{"release":"2010-05-31T11:19:01Z","modification":"2018-11-29T11:19:01Z","creation":"2018-11-29T11:19:01Z"},"accession":"S-JCBD-200910095","cross_references":{"doi":["10.1083/jcb.200910095"]}}