bioimagesUnknownAzusa HottaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200910095JCB

MCF-10Aeco cells expressing EB1-GFP and RFP-LL5α were cocultured with parental non-fluorescent cells on glass-bottomed dishes (fluorescent:non-fluorescent cells=1:10). Z-scanning images from the apical (bottom of the Z-axis here) to basal planes (top of the Z axis) of the cell were collected using a Revolution XD system.

MCF-10Aeco cells expressing GFP-α-tubulin and RFP-LL5α were seeded on glass-bottomed dishes at a low density and cultured overnight. Time-lapse images of the cells were collected at 2.5-min intervals for 8 h 6 min using a DeltaVision Core system. During the time-lapse imaging, the cells divided into two daughter cells. The RFP-LL5α patches disappear when the cells become detached from the glass at the onset of cell division, and reappear soon after the cells adhere to the glass. Pixel Size: z=0.127, y=0.127 m; Time intervals: 2.5 min.

Mouse mammary tissues were immunostained for b-catenin (Cy2, 488 nm laser), LL5α (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.09, y=0.09 um.

MCF-10A cells were fixed and stained for LL5s (Cy5, 633 nm laser), integrin a3 (rhodamine red-X, 543 nm laser) and integrin a6 (Cy2, 488 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.04, y=0.04 um.

Mouse small intestine were immunostained for laminin (Cy2, 488 nm laser), LL5a (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.45, y=0.45 um.

MCF-10A cells were fixed and immunostained for LL5b (Cy5, 633 nm laser), laminin-5 (rhodamine red-X, 543 nm laser) and integrin a6 (Cy2, 488 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.07, y=0.07 um.

MCF-10A cells were fixed and stained for LL5s (Cy5, 633 nm laser), laminin-5 (Cy2, 488 nm laser) and integrin a3 (rhodamine red-X, 543 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.04, y=0.04 um.

At 3 h after seeding on coverslips, MCF-10Aeco cells were fixed and immunostained for tubulin (Cy2, 488 nm laser), EB1 (rhodamine red-X, 543 nm laser) and LL5s (Cy5, 633 nm laser), and observed using a LSM510 confocal laser-scanning microscope system.

Mouse mammary tissues were immunostained for laminin (Cy2, 488 nm laser), LL5a (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system. Pixel Size: z=0.09, y=0.09 um.

bioimagesFigure 3 - DcFigure 3 - DbImage 16728 (Figure 3 - Db)Figure 3 - DaFigure 8 - CImage 16799 (Figure 1 - Dc)Figuer 4 - BaImage 16765 (Figuer 4 - Bb)Figuer 4 - BbVideo 4 - NoneImage 16710 (Figure 3 - Da)Figure 1 - DcFiguer 4 - DFigure 8Figuer 4Image 16772 (Figuer 4 - D)Image 16751 (Figuer 4 - Ba)Image 16735 (Figure 3 - Dc)Video 4Figure 1Image 17585 (Figure 8 - C)Image 16705 (Video 4 - None)Figure 3Tomomi KawakatsuTomoya NakataniTomoko HamajiTaiko SukezaneAzusa HottaChiyuki MatsuiAnna AkhmanovaToshitaka SatoYuko Mimori-KiyosueTsuyoshi AkagiYoshimi TakaiIlya GrigorievfalseLaminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5α/β2010-05-31T11:19:01Z2018-11-29T11:19:01Z2018-11-29T11:19:01ZS-JCBD-20091009510.1083/jcb.200910095