bioimagesUnknownSimona GiuntaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200911156JCBAnti-Chk2 immunoblotScans of WB of PCNA loading control.see figure 4CCells pre-incubated with ATMi and irradiated with 2 Gy. Images were acquired on Radiance confocal microscope using 60x lens and 1x zoom.IR-treated, asynchronously growing HeLA cells co-stained with γH2AX (red) and H3 phospho-serine 10 (green) antibodies, and for DNA (DAPI, blue). Cells pre-incubated with DMSO (no PIKK inhibitors) and irradiated with 2 Gy. Images were acquired on Radiance confocal microscope using 60x lens and 2x zoom.MDC1 co-localizes with γH2AX foci in different mitotic stages of U2OS cells after IR. U2OS cells in prophase were co-stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Immunoblot of SMC1 phosphorylated on S966Anti-BRCA1 (top) and anti-RNF168 (bottom) immunoblots: long exposure.Untreated, asynchronously growing U2OS cells co-stained for 53BP1 (green), γH2AX (red), and DNA (DAPI, blue).Untreated U2OS cells synchronized in mitosis were co-stained for 53BP1 (green) and γH2AX (red) and DNA (DAPI, blue).MDC1 co-localizes with γH2AX foci in different mitotic stages of U2OS cells after IR. U2OS cells in anaphase were co-stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Phleomycin-treated, asynchronously growing U2OS cells co-stained with MDC1 (red) and H3 phospho-serine 10 (green) antibodies and for DNA (DAPI, blue).U2OS cells were incubated with DMSO in presence of nocodazole block and treated with IR. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 24 hours.timecourse of cells released from Thymidine/Nocodazole arrestWestern blot of MDC1 following peptide pull-downs with unmodified and phosphorylated H2AX C-terminal peptides.immunoblot of KAP1 and KAP1 phosphorylated on S284 (ATM-dependent site)U2OS cells were incubated with DMSO in presence of nocodazole block. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 2 hours.Anti-53BP1 immunoblotIR-treated (IR) U2OS cells were released from nocodazole arrest and fixed after 5 hours. Cells were co-stained for 53BP1 (green), γH2AX (red), and DNA (DAPI, blue).Phleomycin-treated, asynchronously growing HeLa cells co-stained with MDC1 (red) and H3 phospho-serine 10 (green) antibodies and for DNA (DAPI, blue).Asynchronously growing U2OS cells were pre-treated with a Chk1 inhibitor for 1 hour and co-treated with phleomycin in presence of the Chk1 inhibitor for 2 more hours. Cells were co-stained with 53BP1 (green) and γH2AX (red) antibodies and for DNA (blue).Anti-NBS1 immunoblot.KAP1 and KAP1pS284Immunoblot of histone H4K20me2 and H3K79me2U2OS cells were incubated with DMSO in presence of nocodazole block and treated with IR. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 1 hour.Asynchronously growing, IR-treated, U2OS cells, stably expressing GFP-RNF8 (green) and co-stained with γH2AX antibodies (red) and for DNA (DAPI, blue).U2OS cells were incubated with DMSO in presence of nocodazole block. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 4 hours.Cells pre-incubated with the combination of ATMi and DNA-PKi and irradiated with 10 Gy. Images were acquired on Radiance confocal microscope using 60x lens and 1x zoom.Asynchronously growing U2OS cells were treated with a Chk1 inhibitor for 3 hours. Cells were co-stained with 53BP1 (green) and γH2AX (red) antibodies and for DNA (DAPI, blue).Phleomycin treated, asynchronously growing U2OS cells co-stained with 53BP1 and H3 phospho-serine 10 antibodies.Immunoblot of Chk1 phosphorylated on S345timecourse after release from nocodazoleU2OS cells were incubated with DMSO in presence of nocodazole block and treated with IR. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 30 minutes.U2OS cells were incubated with ATM and DNA-PK inhibitors in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 30 minutes.Untreated, asynchronously growing HeLa cells co-stained with MDC1 (red) and H3 phospho-serine 10 (green) antibodies and for DNA (DAPI, blue).IR-treated (IR) U2OS cells were released from nocodazole arrest and fixed after 10 hours. Cells were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).γH2AX staining of mitotic chromosomes co-stained for histone H3pS10 in UNTREATED samples. All experiments were performed with U2OS cells. Cells were stained for gamma-H2AX (red), H3ps10 (green) and DNA (DAPI, blue).53BP1 is excluded from IRIF during mitosis. IR-treated asynchronously growing U2OS cells were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).see fig 4CUntreated, asynchronously growing U2OS cells were co-stained with 53BP1 (green) and γH2AX (red) antibodies and for DNA (DAPI, blue).Immunoblot of histone H2AX phosphorylated on S139.U2OS cells were pre-incubated with DMSO (no PIKK inhibitors) and irradiated with 10 Gy (supplemental data). Cells were stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Anti-53BP1 (top) and anti-RNF8 (bottom) immunoblot: long exposureU2OS cells stably expressing GFP-RNF8 (green) were fixed and stained with anti-tubulin antibody (red) and DAPI. Images were acquired on Radiance confocal microscope using 60x lens and 4x zoom.53BP1 is excluded from IRIF during mitosis. Untreated asynchronously growing U2OS were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).Asynchronously growing, UNTREATED, U2OS cells co-stained with BRCA1 (green), γH2AX antibodies (red), and for DNA (DAPI, blue).Asynchronously growing U2OS cells were treated with phleomycin for 2 hours. Cells were co-stained with 53BP1 (green) and γH2AX (red) antibodies and for DNA (DAPI, blue).Anti-BRCA1 (top), anti-RNF168 (middle) and anti-alpha-actin (bottom) immunoblot: short exposure.Anti-histone H2AX immunoblot. Cell lysates were resolved on 15% SDS-PAGE. See Figure 1F for description.U2OS cells were incubated with ATM and DNA-PK inhibitors and treated with IR in presence of nocodazole block. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI.Immunoblot of 53BP1 phosphorylated on S25Untreated, asynchronously growing BJ primary fibroblasts co-stained with γH2AX (red)and H3 phospho-serine 10 (green) antibodies, and for DNA (DAPI, blue). RNF8 has altered mobility in mitosisTime-lapse imaging was performed on a Zeiss Axiovert 200 M inverted microscope using LSM510 software. Images were acquired at 1.4x zoom (shown as Movie 1 in the corresponding manuscript).Untreated U2OS cells were released from nocodazole arrest and fixed after 1 hour. Cells were co-stained for 53BP1 (green), γH2AX (red), and DNA (DAPI, blue).Mitotic U2OS cells were obtained by shake-off and attached to coverslips by centrifugation using a cytospin centrifuge at 1800 rpm. Images were acquired on a Radiance confocal microscope with 60x lens and 4x zoom. Cells were stained for gamma-H2AX (Red), NBS1 (Green) and DNA (DAPI, blue).Time-lapse imaging was performed on a Zeiss Axiovert 200 M inverted microscope using LSM510 software. Images were acquired at 1.8x zoom. (Shown as Movie 3 in the corresponding manuscript). IR-treated (IR) U2OS cells were released from nocodazole arrest and fixed after 30 minutes. Cells were co-stained for 53BP1 (green) and γH2AX (red) and DNA (DAPI, blue). Phleomycin-treated, asynchronously growing BJ primary fibroblasts co-stained with γH2AX (red) and H3 phospho-serine 10 (green) antibodies, and for DNA (DAPI, blue).U2OS cells were incubated with DMSO in presence of nocodazole block and treated with IR. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 2 hours.Asynchronously growing U2OS cells treated with IR co-stained with FK2 (red) and γH2AX (green) antibodies and for DNA (DAPI, blue).IR-treated (IR) U2OS cells were released from nocodazole arrest and fixed after 15 hours. Cells were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).U2OS cells were pre-treated with ATMi and irradiated with 10 Gy (supplemental data). Cells were stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Cells pre-incubated with ATMi and irradiated with 10 Gy. Images were acquired on Radiance confocal microscope using 60x lens and 1x zoom.Anti-NBS1 immunoblotU2OS cells were incubated with ATM and DNA-PK inhibitors in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 2 hours.U2OS cells were incubated with ATM and DNA-PK inhibitors in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 24 hours.timecourseU2OS cells were pre-incubated with the combination of ATMi and DNA-PKi and irradiated with 10 Gy (supplemental data). Cells were stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).U2OS cells were incubated with ATM and DNA-PK inhibitors and treated with IR in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 2 hours.Immunoblot of ATM phosphorylated on S1981.Untreated U2OS cells were released from nocodazole arrest and fixed after 10 hours. Cells were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).Untreated U2OS cells were released from nocodazole arrest and fixed after 48 hours. Cells were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).Asynchronous U2OS cells were irradiated with 2 Gy. 30 minutes later mitotic cells were harvested by shake-off and centrifuged onto coverslips using a cytospin centrifuge at 1800 rpm. Images were acquired on a Radiance confocal microscope with 60x lens and 4x zoom. Cells were stained for gamma-H2AX (Red), NBS1 (Green) and DNA (DAPI, blue).U2OS cells were incubated with DMSO in presence of nocodazole block. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 1 hour.Unirradiated control cells pre-incubated with ATMi. Images were acquired on Radiance confocal microscope using 60x lens and 1x zoom.IR-treated, asynchronously growing U2OS cells co-stained with 53BP1 and H3 phospho-serine 10 antibodies.U2OS cells were incubated with ATM and DNA-PK inhibitors in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 1 hour.U2OS cells were incubated with ATM and DNA-PK inhibitors in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 4 hours.Cells pre-incubated with the combination of ATMi and DNA-PKi and irradiated with 2 Gy. Images were acquired on Radiance confocal microscope using 60x lens and 1x zoom.Asynchronously growing U2OS cells were treated with phleomycin and co-stained for 53BP1 (green), γH2AX (red), and DNA (DAPI, blue). Shows 53BP1 exclusion from IRIF during mitosis.immunoblot of SMC1 (loading control)MDC1 is constitutively phosphorylated on the TQXF motifs in mitosisAsynchronously growing IR-treated U2OS cells, stably expressing GFP-MDC1 (green) and co-stained with γH2AX antibodies (red) and for DNA (DAPI, blue).U2OS cells synchronized in mitosis by thymidine-nocodazole block, treated with ATM and DNA-PK inhibitors and stained for γH2AX, 53BP1 and DAPI.Immunoblot of DNA-PKCs phosphorylated on T2609.Untreated, asynchronously growing BJ primary fibroblasts co-stained with MDC1 (red) and H3 phospho-serine 10 (green) antibodies and for DNA (DAPI, blue).Asynchronously growing, UNTREATED, U2OS cells, stably expressing GFP-RNF8 (green) and co-stained with γH2AX antibodies (red) and for DNA (DAPI, blue).Untreated U2OS cells synchronized in mitosis by thymidine-nocodazole block and stained for γH2AX, 53BP1 and DAPI.Untreated U2OS cells were released from nocodazole arrest and fixed after 5 hours. Cells were co-stained for 53BP1 (green), γH2AX (red), and DNA (DAPI, blue).Untreated, asynchronously growing HeLA cells were co-stained with γH2AX (red)and H3 phospho-serine 10 (green) antibodies, and for DNA (DAPI, blue).Immunoblot of Chk2 phosphorylated on T68U2OS cells were incubated with DMSO in presence of nocodazole block. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 30 minutes.Untreated, asynchronously growing U2OS cells co-stained with 53BP1 and H3 phospho-serine 10 antibodies.IR-treated (IR) U2OS cells were released from nocodazole arrest and fixed after 24 hours. Cells were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).IR-treated, asynchronously growing BJ primary fibroblasts co-stained for 53BP1 and γH2AX.U2OS cells were incubated with ATM and DNA-PK inhibitors and treated with IR in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 24 hours.Anti-53BP1 (top) and anti-RNF8 (bottom) immunoblot: short exposure.timecourse after nocodazole releaseIR-treated, asynchronously growing BJ primary fibroblasts co-stained with 53BP1 and H3 phospho-serine 10 antibodies.IR-treated (IR) U2OS cells were released from nocodazole arrest and fixed after 1 hour. Cells were co-stained for 53BP1 (green), γH2AX (red), and DNA (DAPI, blue).U2OS cells were incubated with ATM and DNA-PK inhibitors and treated with IR in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 30 minutes.Anti-SMC1 immunoblotUnirradiated control U2OS cells were pre-treated with ATMi. Cells were stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Untreated U2OS cells were released from nocodazole arrest and fixed after 15 hours. Cells were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).Untreated U2OS cells were released from nocodazole arrest and fixed after 2 hours. Cells were co-stained for 53BP1 (green), γH2AX (red), and DNA (DAPI, blue).Anti-Chk1 immunoblotUntreated, asynchronously growing MRC5 primary fibroblasts co-stained with 53BP1 and γH2AX antibodies.MDC1 co-localizes with γH2AX foci in different mitotic stages of U2OS cells after IR. U2OS cells in prometaphase were co-stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Unirradiated control U2OS cells were pre-incubated with the combination of ATMi and DNA-PKi. Cells were stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Phleomycin-treated, asynchronously growing BJ primary fibroblasts co-stained with MDC1 (red) and H3 phospho-serine 10 (green) antibodies and for DNA (DAPI, blue).Time-lapse imaging was performed on a Zeiss Axiovert 200 M inverted microscope using LSM510 software. Images were acquired at 1.8x zoom. (Shown as Movie 2 in the corresponding manuscript).Immunoblot of SMC1 phosphorylated on S966.IR-treated, asynchronously growing MRC5 primary fibroblasts co-stained with γH2AX (red)and H3 phospho-serine 10 (green) antibodies, and for DNA (DAPI, blue).Asynchronously growing, IR-treated, U2OS cells, stably expressing GFP-RNF168 (green) and co-stained with γH2AX antibodies (red) and for DNA (DAPI, blue).Asynchronously growing, UNTREATED, U2OS cells, stably expressing GFP-RNF168 (green) and co-stained with γH2AX antibodies (red) and for DNA (blue).Immunoblots of histone H3 phosphorylated on S10 and ubiquitylated histone H2A. Cell lysates were resolved on 15% SDS-PAGE. See Figure 1F for description.Asynchronous U2OS cell stably expressing GFP-RNF8 stained for CENP-F and DAPI. RNF8 co-localizes with CENP-F at kinetochores in mitosis.U2OS cells synchronized in mitosis by thymidine-nocodazole block, treated with IR and stained for γH2AX, 53BP1 and DAPI.Untreated U2OS cells were released from nocodazole arrest and fixed after 24 hours. Cells were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).Anti-phospho-H2AX immunoblot.Cells pre-incubated with DMSO (no PIKK inhibitors) and irradiated with 10 Gy. Images were acquired on Radiance confocal microscope using 60x lens and 2x zoom.U2OS cells were incubated with ATM and DNA-PK inhibitors and treated with IR in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 1 hour. IR-treated, asynchronously growing BJ primary fibroblasts co-stained with γH2AX (red)and H3 phospho-serine 10 (green) antibodies, and for DNA (DAPI, blue).Control for Figure 2d: time-lapse imaging of the unirradiated control cellsAsynchronously growing U2OS cells treated with IR co-stained with FK2 (red)and γH2AX antibodies (green) and for DNA (DAPI, blue).U2OS cells were incubated with DMSO in presence of nocodazole block. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 24 hours.Unirradiated control cells pre-incubated with the combination of ATMi and DNA-PKi. Images were acquired on Radiance confocal microscope using 60x lens and 1x zoom.Untreated asynchronously growing U2OS cells co-stained for 53BP1 (green), γH2AX (red), and DNA (DAPI, blue).Asynchronously growing U2OS cells were treated with phleomycin and co-stained for 53BP1 (green), γH2AX (red), and DAPI (blue).IR-treated (IR) U2OS cells were released from nocodazole arrest and fixed after 2 hours. Cells were co-stained for 53BP1 (green), γH2AX (red), and DNA (DAPI, blue).Unirradiated control U2OS cells pre-incubated with DMSO (no PIKK inhibitors). Cells were stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Unirradiated control cells pre-incubated with DMSO (no PIKK inhibitors). Images were acquired on Radiance confocal microscope using 60x lens and 2x zoomU2OS cells were pre-treated with ATMi and irradiated with 2 Gy. Cells were stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Anti-ATM immunoblot.Asynchronously growing U2OS cells treated with phleomycin were co-stained with BRCA1 (green), γH2AX antibodies (red) and DNA (DAPI, blue).Scans of WB of Chk2 phospho-threonine 68.IR-treated, asynchronously growing MRC5 primary fibroblasts co-stained with 53BP1 and γH2AX antibodies.IR-treated (IR) U2OS cells were released from nocodazole arrest and fixed after 48 hours. Cells were co-stained for 53BP1 (green), γH2AX (red) and DNA (DAPI, blue).U2OS cells were incubated with DMSO in presence of nocodazole block and treated with IR. Nocodazole was washed out and cells were released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 4 hours.IR-treated, asynchronously growing U2OS cells co-stained for 53BP1 and γH2AX.U2OS cells were pre-incubated with DMSO (no PIKK inhibitors) and irradiated with 2 Gy. Cells were stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Untreated, asynchronously growing HeLa cells co-stained for 53BP1 and γH2AX.U2OS cells were pre-incubated with the combination of ATMi and DNA-PKi and irradiated with 2 Gy. Cells were stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).IR-treated (IR) U2OS cells synchronized in mitosis were co-stained 53BP1 (green), γH2AX (red), and DNA (DAPI, blue).anti-NBS1 and anti-Chk2 pT68 immunoblot. NBS1 provides loading control.γH2AX staining of mitotic chromosomes co-stained for histone H3pS10 in IR-treated samples. All experiments were performed with U2OS cells. Cells were stained for gamma-H2AX (red), H3ps10 (green) and DNA (DAPI, blue).Untreated U2OS cells were released from nocodazole arrest and fixed after 30 minutes. Cells were co-stained for 53BP1 (green) and γH2AX (red) and DNA (DAPI, blue).Anti-histone H4 immunoblot. Cell lysates were resolved on 15% SDS-PAGE. See Figure 1F for description.U2OS cells were incubated with ATM and DNA-PK inhibitors and treated with IR in presence of nocodazole block. Nocodazole and inhibitors were washed out and cells released into fresh medium. Cells were fixed and co-stained for γH2AX, 53BP1 and DAPI after 4 hours.Untreated, asynchronously growing MRC5 primary cells co-stained with γH2AX (red)and H3 phospho-serine 10 (green) antibodies, and for DNA (DAPI, blue).MDC1 co-localizes with γH2AX foci in different mitotic stages of U2OS cells after IR. U2OS cells in telophase were co-stained for gamma-H2AX (red), MDC1 (green) and DNA (DAPI, blue).Untreated, asynchronously growing U2OS cells were co-stained with MDC1 (red) and H3 phospho-serine 10 (green) antibodies and for DNA (DAPI, blue).γH2AX staining of mitotic chromosomes co-stained for histone H3pS10 in samples treated with phleomycin (supplemental data). All experiments were performed with U2OS cells. Cells were stained for gamma-H2AX (red), H3ps10 (green) and DNA (DAPI, blue).bioimagesImage 10225 (Figure 1 - B)Image 10124 (Figure 1 - A)Image 10249 (Figure 3 - D)Image 10132 (Figure 2 - C)Image 10136 (Figure 3 - B)Image 18060 (Figure 3 - H)Image 10202 (Figure 5 - A)Image 10151 (Figure 4 - B)Image 10333 (SupFigure 2 - F)Image 10178 (SupFigure 3 - B)Image 18057 (SupFigure 2 - H)Image 10204 (Figure 2 - B)Image 10197 (Figure 5 - A)Image 10248 (Figure 3 - F)Image 14452 (Figure 5 - A)Image 10211 (SupFigure 1 - A)Image 10190 (Figure 5 - A)Image 10213 (SupFigure 3 - A)Image 10230 (Figure 1 - B)Image 10128 (Figure 2 - A)Image 14451 (Figure 5 - A)Image 10332 (SupFigure 2 - F)Image 10191 (Figure 5 - A)Image 18058 (SupFigure 2 - H)Image 10173 (SupFigure 1 - C)Image 10179 (SupFigure 3 - B)Image 10174 (SupFigure 1 - D)Image 10331 (SupFigure 2 - F)Image 10214 (SupFigure 3 - B)Image 10231 (Figure 1 - B)Figure 3 - DImage 10253 (Figure 3 - F)Figure 3 - EImage 10123 (Figure 1 - A)Figure 3 - FFigure 3 - 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B)Image 10152 (Figure 4 - B)Image 10247 (Figure 3 - F)Image 10156 (Figure 4 - C)Image 10246 (Figure 3 - F)Image 10226 (Figure 1 - B)Image 10141 (Figure 4 - B)Image 14453 (Figure 5 - A)SupFigure 2 - HSupFigure 2 - GImage 10160 (SupFigure 1 - A)Image 10167 (SupFigure 1 - B)Image 10209 (Figure 4 - B)Image 10186 (Figure 5 - A)Image 10125 (Figure 1 - C)Image 10129 (Figure 2 - B)SupFigure 2 - FImage 10254 (Figure 4 - A)Image 14429 (Figure 4 - C)Image 10222 (Figure 1 - D)SupFigure 2 - AImage 10257 (Figure 4 - A)Image 10229 (Figure 1 - B)Image 10198 (Figure 5 - A)SupFigure 1 - DSupFigure 1 - CSupFigure 1 - BSupFigure 1 - AFigure 4 - AFigure 4 - BImage 10261 (Figure 4 - A)Image 10171 (SupFigure 1 - C)Figure 4 - CImage 10137 (Figure 3 - C)Image 10145 (Figure 4 - B)Image 10250 (Figure 3 - D)Image 10133 (Figure 3 - A)Image 14424 (SupFigure 2 - H)Image 10194 (Figure 5 - A)Image 14430 (Figure 4 - C)Image 18054 (Figure 4 - C)Image 10175 (SupFigure 3 - A)Image 10336 (SupFigure 2 - G)Image 10238 (SupFigure 2 - 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A)Image 10131 (Figure 2 - C)Image 10180 (SupFigure 3 - B)Stephen P. JacksonSimona GiuntaRimma BelotserkovskayafalseDNA damage signaling in response to double-strand breaks during mitosis2010-07-26T11:19:08Z2018-11-29T11:19:08Z2018-11-29T11:19:08ZS-JCBD-20091115610.1083/jcb.200911156