bioimagesUnknownIoannis P. NezisThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201002035JCB

dBruce colocalizes with the autophagic marker Atg8a-GFP and is degraded by autophagy in the nurse cells during late oogenesis. Confocal micrographs of late stage egg chambers expressing Atg8a-GFP and stained for dBruce at early stage 12. Note the colocalization between Atg8a-GFP and dBruce in punctuate structures during stage 12.

51μm z stack<br /><br />Autophagy is activated in dying nurse cells during late oogenesis in Drosophila melanogaster. <br />(A-C) Confocal micrographs of egg chambers of flies expressing the UASp-GFP-mCherry-DrAtg8a transgene exclusively in the germline (genotype: UASp-GFPmCherry-DrAtg8a /+; nanos-VP-16 Gal4/+). Expression of UASp-GFP-mCherry-DrAtg8a exhibits punctate staining pattern (arrows). Note that during developmental stages early 12 (A) and late 12 (B) the punctate dots are yellow (merge panel). In late stage 13 (C) large red dots are evident indicating the presence of acidic compartments (autolysosomes). The arrowhead in (A) points to the physical connection between the oocyte and the nurse cells during early stage 12 (not observed during late stage 12).<br />

Autophagy is activated in dying nurse cells during late oogenesis in Drosophila melanogaster. <br />(E) Transmission electron micrographs of nurse cells in in stage 13 egg chambers. Autophagosomes and autolysosomes are evident in the remaining nurse cell cytoplasm (inset,<br />Arrow in figure). Note that the autolysosome shown contains condensed material resembling the material of the condensed and fragmented nurse cell nucleus (NCN), providing evidence for nuclear autophagy.<br />

Genetic inhibition of autophagy in the germ line prevents DNA fragmentation.<br />Confocal micrographs of stage 13/14 egg chambers after TUNEL staining. <br />(C-E) stage 14 atg1-/- (C), atg13-/- (D) and vps34-/- (E) germline mutant chambers have persisting nurse cell nuclei that do not contain fragmented DNA (negative TUNEL staining).<br />

Germline autophagy mutant egg chambers exhibit significantly reduced expression of cleaved caspase-3. Confocal micrographs of stage 13/14 egg chambers stained for cleaved caspase-3. (A) Wild type late stage 13 egg chamber showing cleaved caspase-3 staining in the degenerating nurse cell cluster.

21 μm z stack<br /><br />Autophagy is activated in dying nurse cells during late oogenesis in Drosophila melanogaster. <br />(A-C) Confocal micrographs of egg chambers of flies expressing the UASp-GFP-mCherry-DrAtg8a transgene exclusively in the germline (genotype: UASp-GFPmCherry-DrAtg8a /+; nanos-VP-16 Gal4/+). Expression of UASp-GFP-mCherry-DrAtg8a exhibits punctate staining pattern (arrows). Note that during developmental stages early 12 (A) and late 12 (B) the punctate dots are yellow (merge panel). In late stage 13 (C) large red dots are evident indicating the presence of acidic compartments (autolysosomes). The arrowhead in (A) points to the physical connection between the oocyte and the nurse cells during early stage 12 (not observed during late stage 12).<br />

54 μm z stack<br /><br />Autophagy is activated in dying nurse cells during late oogenesis in Drosophila melanogaster. <br />(A-C) Confocal micrographs of egg chambers of flies expressing the UASp-GFP-mCherry-DrAtg8a transgene exclusively in the germline (genotype: UASp-GFPmCherry-DrAtg8a /+; nanos-VP-16 Gal4/+). Expression of UASp-GFP-mCherry-DrAtg8a exhibits punctate staining pattern (arrows). Note that during developmental stages early 12 (A) and late 12 (B) the punctate dots are yellow (merge panel). In late stage 13 (C) large red dots are evident indicating the presence of acidic compartments (autolysosomes). The arrowhead in (A) points to the physical connection between the oocyte and the nurse cells during early stage 12 (not observed during late stage 12).<br />

bioimagesImage 13165 (Figure 2 - D)Figure 1 - AFigure 1 - EFigure 3 - AImage 13148 (Figure 1 - A)Figure 1 - CImage 13150 (Figure 1 - C)Figure 1 - BFigure 4Figure 4 - BImage 13164 (Figure 2 - C)Image 13167 (Figure 3 - A)Figure 1Figure 2 - CFigure 2 - DImage 13149 (Figure 1 - B)Image 13138 (Figure 1 - E)Figure 3Figure 2Image 13168 (Figure 4 - B)Andreas BrechTrond LamarkIoannis P. NezisGeir BjørkøyBhupendra V. ShravageAntonia P. SagonaHarald StenmarkTerje JohansenTor Erik RustenEric H. BaehreckefalseAutophagic degradation of dBruce controls DNA fragmentation in nurse cells during lateDrosophila melanogasteroogenesis2010-08-16T11:19:44Z2018-11-29T11:19:44Z2018-11-29T11:19:44ZS-JCBD-20100203510.1083/jcb.201002035