{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Alejo E. Rodriguez-Fraticelli"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201002047"],"attach_to":["JCB"],"legend":["Cells were transfected with Cdc42 siRNAs and plated to form cysts for 72 h in the presence (Cdc42V12 repressed) or the absence (induced) of doxycycline (20 μmol). Cells were stained to detect actin (red), Cdc42V12 myc (green) and β-catenin (blue).","Cdc42 and ITSN2 KD cells were plated to form cysts for 72 h. Cells were stained to detect gp135 (red, left), β-catenin (green) and nuclei (blue).","Cells expressing vhITSN2 (green) were stained with pericentrin (centrosomal marker, red) and acetylated tubulin (blue).","Cells expressing vhITSN2 (green, left panels) were stained with acetylated tubulin (red, middle panels) and chromatin (blue, right panels and merge). Bottom panels show the magnification image of the square area indicated in the top panels. Scale bars, 5 μm.","Cells expressing Ct-LGN myc were plated to form cysts for 72 h in the presence (Ct-LGN myc repressed) or the absence (Ct-LGN myc induced) of doxycycline (20 μmol). Cells were stained to detect acetylated tubulin (red), myc (green) and chromatin (blue).","Effect of Cdc42 and ITSN2 siRNA in spindle orientation. Cells were transfected with ITSN2 siRNA pool , Cdc42 siRNA or control siRNA ) and plated to form cysts for 72 hr. Cells were stained to detect actin (green), acetylated tubulin (red) and chromatin (blue).","MDCK cells stably expressing Cdc42-GFP were silenced with siRNA oligos to ITSN2 (right panel) or control (left panel) and plated to form cysts for 24h. Cells with Cdc42-GFP (green) were stained for acetylated tubulin (red) and chromatin (blue).","Cells expressing vhITSN2 (green) were plated to form cysts for 72 hr. Cysts were stained with gp135 (red), and b-catenin (blue).","Mock shRNA expressing MDCK cells (top panels) and 2.3 ITSN2 shRNA MDCK (bottom panels) cells were plated to form cysts for 72 and 120 h and stained for detecting gp135 (red), β-catenin (green), ZO-1 (purple) and nuclei (blue)."],"repository":["bioimages"],"figure_sub":["Figure4 - Panel B","Image 14967 (Figure 2 - panel C)","Image 14964 (Figure7 - panel E)","Figure3 - panel E","Image 15158 (Figure1 - panel B)","Image 15164 (Figure1 - panel B)","Image 14971 (Figure4 - Panel B)","Image 14963 (Figure6 - panel D)","Image 14974 (Figure1 - panel G)","Image 15166 (Figure1 - panel B)","Figure 2 - panel B","Figure 2 - panel A","Figure 2 - panel C","Image 14973 (Figure4 - Panel B)","Figure7 - panel E","Image 14966 (Figure3 - panel E)","Image 14968 (Figure 2 - panel A)","Image 14969 (Figure 2 - panel B)","Image 15159 (Figure1 - panel B)","Figure3","Figure1","Figure7","Image 14972 (Figure4 - Panel B)","Figure6","Figure4","Figure6 - panel D","Image 14962 (Figure6 - panel D)","Figure1 - panel B","Image 14965 (Figure3 - panel E)","Figure1 - panel G","Figure 2"],"pubmed_authors":["Miguel A. Alonso","Silvia Vergarajauregui","Fernando Martín-Belmonte","Alejo E. Rodriguez-Fraticelli","Keith Mostov","Anirban Datta","Dennis J. Eastburn"],"additional_accession":[]},"is_claimable":false,"name":"The Cdc42 GEF Intersectin 2 controls mitotic spindle orientation to form the lumen during epithelial morphogenesis","description":null,"dates":{"release":"2010-05-17T11:19:47Z","modification":"2018-11-29T11:19:47Z","creation":"2018-11-29T11:19:47Z"},"accession":"S-JCBD-201002047","cross_references":{"doi":["10.1083/jcb.201002047"]}}