{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Sarah J. Heasman"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201002067"],"attach_to":["JCB"],"legend":["Confocal z-stack images of cell tracker dye-labelled (pink) T-LB cell migrating on endothelial cells, fixed and labelled for endogenous RhoA (green).","Confocal z-stack images of cell tracker dye-labelled (pink) CEM T cell transmigrating through endothelial cells, fixed and labelled for endogenous RhoA (green).","CEM T cells were imaged by timelapse microscopy 72 h after transfection with control siRNA. Top panels: a polarized control cell is shown migrating across the endothelium extending a lamellipodium at the front (arrow) and with a uropod at the rear. 1 frame was taken every second.","Confocal z-stack images of cell tracker dye-labelled (pink) CEM T cell migrating on endothelial cells, fixed and labelled for endogenous RhoA (green).","Movie of CEM T cells crawling on and transmigrating across endothelial cells, 72 h after transfection with RhoA siRNA. 1 frame was taken every minute.","CEM T cells were fixed 30 min after addition to activated endothelial cells and stained with antibodies to p-MLC and with phalloidin to localize F-actin.","CEM T cells crawling on endothelial cells, 72h after transfection with RhoA siRNA.  Cell has elongated tail at the rear. 1 frame was taken every second.","Movie (time lapse) of CEM T cells crawling on and transmigrating across endothelial cells, 72 h after transfection with control siRNA. The Y axis represents time. 1 frame was taken every minute.","CEM T cell on endothelial cells, 72 h after transfection with RhoA siRNA. Cell shown has rounded cell body and two protrusions, extending from opposite sides of the cell body. One frame was taken every second.","Image shows a CEM T cell labelled with cell tracker dye that has been added to activated EC monolayers. After 30 min, cells were fixed and stained with antibodies to GEF-H1.","Confocal z-stack images of cell tracker dye-labelled (pink) T-LB cell transmigrating through endothelial cells, fixed and labelled for endogenous RhoA (green)."],"repository":["bioimages"],"figure_sub":["Image 13102 (Figure 1 - f)","Figure 1 - f","Image 13107 (Figure 3 - a)","Image 13114 (Figure 5 - f)","Image 13105 (Figure 3 - a)","Figure 5 - f","Image 13104 (Figure 2 - C)","Image 13162 (Figure 2 - C)","Image 13111 (Figure 4 - j)","Image 13108 (Figure 3 - a)","Figure 3 - a","Figure 5","Image 13106 (Figure 3 - a)","Figure 4","Image 13103 (Figure 1 - f)","Image 13113 (Figure 5 - f)","Image 13112 (Figure 4 - j)","Figure 4 - j","Image 13110 (Figure 4 - j)","Image 13163 (Figure 2 - C)","Figure 1","Figure 2 - C","Figure 3","Figure 2"],"pubmed_authors":["Susan Cox","Leo M. Carlin","Anne J. Ridley","Sarah J. Heasman","Tony Ng"],"additional_accession":[]},"is_claimable":false,"name":"Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration","description":null,"dates":{"release":"2010-08-23T11:19:48Z","modification":"2018-11-29T11:19:48Z","creation":"2018-11-29T11:19:48Z"},"accession":"S-JCBD-201002067","cross_references":{"doi":["10.1083/jcb.201002067"]}}