bioimagesUnknownSarah J. HeasmanThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201002067JCB

Confocal z-stack images of cell tracker dye-labelled (pink) T-LB cell migrating on endothelial cells, fixed and labelled for endogenous RhoA (green).

Confocal z-stack images of cell tracker dye-labelled (pink) CEM T cell transmigrating through endothelial cells, fixed and labelled for endogenous RhoA (green).

CEM T cells were imaged by timelapse microscopy 72 h after transfection with control siRNA. Top panels: a polarized control cell is shown migrating across the endothelium extending a lamellipodium at the front (arrow) and with a uropod at the rear. 1 frame was taken every second.

Confocal z-stack images of cell tracker dye-labelled (pink) CEM T cell migrating on endothelial cells, fixed and labelled for endogenous RhoA (green).

Movie of CEM T cells crawling on and transmigrating across endothelial cells, 72 h after transfection with RhoA siRNA. 1 frame was taken every minute.

CEM T cells were fixed 30 min after addition to activated endothelial cells and stained with antibodies to p-MLC and with phalloidin to localize F-actin.

CEM T cells crawling on endothelial cells, 72h after transfection with RhoA siRNA. Cell has elongated tail at the rear. 1 frame was taken every second.

Movie (time lapse) of CEM T cells crawling on and transmigrating across endothelial cells, 72 h after transfection with control siRNA. The Y axis represents time. 1 frame was taken every minute.

CEM T cell on endothelial cells, 72 h after transfection with RhoA siRNA. Cell shown has rounded cell body and two protrusions, extending from opposite sides of the cell body. One frame was taken every second.

Image shows a CEM T cell labelled with cell tracker dye that has been added to activated EC monolayers. After 30 min, cells were fixed and stained with antibodies to GEF-H1.

Confocal z-stack images of cell tracker dye-labelled (pink) T-LB cell transmigrating through endothelial cells, fixed and labelled for endogenous RhoA (green).

bioimagesImage 13102 (Figure 1 - f)Figure 1 - fImage 13107 (Figure 3 - a)Image 13114 (Figure 5 - f)Image 13105 (Figure 3 - a)Figure 5 - fImage 13104 (Figure 2 - C)Image 13162 (Figure 2 - C)Image 13111 (Figure 4 - j)Image 13108 (Figure 3 - a)Figure 3 - aFigure 5Image 13106 (Figure 3 - a)Figure 4Image 13103 (Figure 1 - f)Image 13113 (Figure 5 - f)Image 13112 (Figure 4 - j)Figure 4 - jImage 13110 (Figure 4 - j)Image 13163 (Figure 2 - C)Figure 1Figure 2 - CFigure 3Figure 2Susan CoxLeo M. CarlinAnne J. RidleySarah J. HeasmanTony NgfalseCoordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration2010-08-23T11:19:48Z2018-11-29T11:19:48Z2018-11-29T11:19:48ZS-JCBD-20100206710.1083/jcb.201002067