{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Mark T. Howes"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201002119"],"attach_to":["JCB"],"legend":["20 mgmL HRP uptake in Cav1-/- MEFs for 15 seconds in the presence of unlablled CTxB.","Fractions 2.8 was fixed onto EM grids and labeled for Cav1.","CTxB-555 and Tf-647 were pulsed into untreated Cav1-/- MEFs for 5 minutes, cells fixed and labeled with CTxB-488 post fixation.","CTxB-HRP was pulsed into Cav1-/-MEFs for 15 seconds, DAB reaction performed and cells HPF, freeze substituted and processed for EM.","WT MEFs were wounded and cells allowed to migrate into wound for 4-8 hours. Cells were pulsed for 2 minutes with CTxB, Tf and anti-CD44 antibodies.","Anti-CD44-HRP was pulsed into Cav1-/- MEFs for 2 minutes, DAB reaction performed and cells fixed and processed for EM.","CTxB-HRP was pulsed into Dyngo4a treated Cav1-/- MEFs for 2 minutes, DAB reaction performed and samples processed for EM.","WT MEFs were wounded and allowed to migrate for 4 hours. CTxB-HRP was pulsed into cells for 2 minutes, DAB reaction performed and cells fixed and processed for EM.","Anti-Thy-1, CTxB-555 and Tf-647 were pulsed into Cav1-/- MEFs for 2 minutes prior to fixation.","CTxB-HRP was added to cells for 5 mintues prior to fractionation. Fraction 2.8 was fixed and adsorbed onto EM grids and DAB reaction performed.","CTxB-555 and Tf-647 were pulsed into Cav1-/- MEFs for 2 minutes prior to fixation and labeling for myoferlin","Fractions 2.8 was fixed onto EM grids and labeled for Flot1.","20 mgmL HRP uptake in Cav1-/- MEFs without any CTxB.","Migrating WT MEFs were treated with Nocodazole for 30 minutes prior to addition of CTxB-555, Tf-647 and anti-CD44 antibodies for 2 minutes.","CTxB-HRP was pulsed into untreated Cav1-/- MEFs for 2 minutes, DAB reaction performed and samples processed for EM.","Anti-CD44-HRP was pulsed into Cav1-/- MEFs for 10 minutes, DAB reaction performed and cells fixed and processed for EM.","WT MEFs were wounded and cells allowed to migrate into the wound for 4-8 hours. Cells were pulsed for 2 minutes with CTxB-555 and Tf-647 and were labeled post fixation for Cav1.","Fractions 2.8 was fixed onto EM grids and labeled for Dynamin.","GFP-GPI cells were treated with wortmannin for 20 minutes prior to addition of anti-GFP antibodies for a further 10 minutes.","Anti-CD44-HRP was pulsed into Cav1-/- MEFs for 40 minutes, DAB reaction performed and cells fixed and processed for EM.","Fractions 2.8 was fixed onto EM grids and labeled for GRAF1.","Migrating WT MEFs were treated with Taxol for 30 minutes prior to addition of CTxB-555, Tf-647 and anti-CD44 antibodies for 2 minutes.","EM of membranes in Fraction 2.8","Anti-CD44, CTxB-555 and Tf-647 were pulsed into Cav1-/- MEFs for 2 minutes prior to fixation.","CTxB-555 and Tf-647 were pulsed into Dyngo4a Cav1-/- MEFs for 5 minutes, cells fixed and labeled with CTxB-488 post fixation."],"repository":["bioimages"],"figure_sub":["Image 13195 (Figure 1 - A)","Image 13324 (Figure 6 - A)","Image 13219 (Figure 3 - B)","Image 13246 (Figure 4 - B)","Image 13207 (Figure 3 - B)","Image 13274 (Figure 4 - G)","Image 13200 (Figure 1 - A)","Image 13383 (Figure 6 - C)","Image 13348 (Figure 1 - G)","Image 13284 (Figure 5 - E)","Image 13404 (Figure 5 - A)","Image 13183 (Figure 1 - A)","Image 13313 (Figure 5 - E)","Image 13301 (Figure 5 - E)","Image 13371 (Figure 6 - C)","Image 13256 (Figure 4 - G)","Image 13296 (Figure 5 - E)","Image 13378 (Figure 6 - C)","Image 13365 (Figure 6 - C)","Image 13268 (Figure 4 - G)","Image 13213 (Figure 3 - B)","Image 13243 (Figure 3 - B)","Image 13224 (Figure 3 - B)","Image 13230 (Figure 3 - B)","Image 13237 (Figure 3 - B)","Image 13290 (Figure 5 - E)","Image 13359 (Figure 4 - D)","Image 13410 (Figure 5 - A)","Image 13330 (Figure 1 - F)","Image 13225 (Figure 3 - B)","Image 13242 (Figure 3 - B)","Image 13377 (Figure 6 - C)","Image 13212 (Figure 3 - B)","Image 13182 (Figure 1 - A)","Image 13236 (Figure 3 - B)","Image 13206 (Figure 3 - B)","Image 13384 (Figure 6 - C)","Image 13335 (Figure 1 - G)","Image 13398 (Figure 5 - B)","Image 13302 (Figure 5 - E)","Figure 3 - B","Image 13178 (Figure 1 - A)","Image 13269 (Figure 4 - G)","Image 13300 (Figure 5 - E)","Image 13273 (Figure 4 - G)","Image 13201 (Figure 1 - A)","Image 13417 (Figure 5 - A)","Image 13309 (Figure 5 - E)","Image 13354 (Figure 1 - G)","Image 13341 (Figure 1 - G)","Image 13189 (Figure 1 - A)","Image 13318 (Figure 6 - A)","Image 13176 (Figure 1 - A)","Image 13337 (Figure 1 - G)","Figure 5","Figure 4","Figure 6","Image 13262 (Figure 4 - G)","Image 13390 (Figure 6 - C)","Image 13394 (Figure 6 - E)","Image 13275 (Figure 4 - G)","Figure 1","Image 13415 (Figure 5 - A)","Figure 3","Image 13307 (Figure 5 - E)","Image 13223 (Figure 3 - B)","Image 13343 (Figure 1 - G)","Image 13260 (Figure 4 - G)","Image 13315 (Figure 5 - E)","Image 13329 (Figure 1 - F)","Image 13281 (Figure 4 - G)","Image 13376 (Figure 6 - C)","Image 13352 (Figure 1 - G)","Image 13188 (Figure 1 - A)","Image 13251 (Figure 4 - G)","Image 13379 (Figure 6 - C)","Image 13232 (Figure 3 - B)","Image 13235 (Figure 3 - 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Hancock","Piers J. Walser","Rumasia Bashir","Robert G. Parton","Alun Jones","Adam McCluskey","Satyajit Mayor","Richard Lundmark","Margaret R. Lindsay","Harvey McMahon","Katia Cortese","Phillip J. Robinson","Gordana Hadzic","Matthew Kirkham","Michelle M. Hill","Fiona Simpson","Libin Liu","Mark T. Howes","Delia J. Hernandez-Deviez","James Riches","Paul Pilch"],"additional_accession":[]},"is_claimable":false,"name":"Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells","description":null,"dates":{"release":"2010-08-16T11:19:54Z","modification":"2018-11-29T11:19:54Z","creation":"2018-11-29T11:19:54Z"},"accession":"S-JCBD-201002119","cross_references":{"doi":["10.1083/jcb.201002119"]}}