bioimagesUnknownYan Shan OngThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201003005JCB

<br />p125A colocalizes extensively with Sec31A.<br /><br />p125A- (red) and Sec31A-(green) positive structures are colocalized stably with each other over time, and undergo homotypic fusion. HeLa cells were transfected to co-express p125A-Cherry and Sec31A-GFP. The cells were subjected to live cell imaging. The vesicular<br />structures of p125A-Cherry colocalize extensively with those of Sec31A-GFP over a<br />period of at least 30 min. This is is also shown as Supplementary Movie 1.

p125A colocalizes extensively with Sec31A.<br /><br />Area of Figure 4D enlarged as in Figure 4E.<br /> <br />p125A- and Sec31A-positive structures are colocalized stably with each other over time, and undergo homotypic fusion. HeLa cells were transfected to co-express p125A-Cherry and Sec31A-GFP. The cells were subjected to live cell imaging. The vesicular<br />structures of p125A-Cherry colocalize extensively with those of Sec31A-GFP over a<br />period of at least 30 min

Exogenous expression of myc-p125A can rescue the dispersed Golgi phenotype in p125A depleted cells. <br /><br />HeLa cells stably expressing GT-GFP were transfected twice with ON-Targetplus SMARTpool siRNA (Dharmacon) against p125A with a 24 hour interval. 48 hour after siRNA transfection, the cells were transfected again to express<br />myc-p125A. At 72 hour after the initial siRNA transfection, the cells were processed for indirect imunofluosecence microscopy to view GT-GFP and myc-p125A.

<br />p125A colocalizes extensively with Sec31A.<br /><br />HeLa cells were fixed and double-labeled with rabbit anti-p125A (red) and mouse anti-Sec31A (green)antibodies.

HeLa cells stably expressing ManII-GFP were depleted of p125A and then double-labeled using mouse anti-GM130 and rabbit anti-GT antibodies<br />followed by viewing the distribution of trans-Golgi marked by GT (red), medial-Golgi<br />marked by ManII-GFP (green) and cis-Golgi marked by GM130 (blue).

bioimagesFigure 6 - Figure 6BFigure 4Figure 6 - Figure 6AFigure 6Image 14348 (Figure 4 - Figure 4D)Image 14347 (Figure 4 - Figure 4E)Figure 4 - Figure 4BFigure 4 - Figure 4DFigure 4 - Figure 4EImage 14346 (Figure 6 - Figure 6B)Image 14312 (Figure 4 - Figure 4B)Image 14311 (Figure 6 - Figure 6A)Yan Shan OngBor Luen TangWanjin HongLi Shen Loofalsep125A exists as part of the mammalian Sec13/Sec31 COPII subcomplex to facilitate ER-Golgi transport2010-08-02T11:20:03Z2018-11-29T11:20:03Z2018-11-29T11:20:03ZS-JCBD-20100300510.1083/jcb.201003005