{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Andrew R. Flannery"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201003021"],"attach_to":["JCB"],"legend":["Figure 7; B and C: Original data represented in the anti-GFP western blots of the immunoprecipitates displayed in figure 7 B and C.  The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63; lane 3, Syt VII (C/S)-YFP/FLAG-CD63; lane 4, Syt VII-YFP; lane 5, SytVII-YFP/FLAG-CD63(Y/A); lane 6, SytVII (C/S)-YFP/FLAG-CD63(Y/A).","Figure 7B: Original data represented in the Ponceau stain of the immunoprecipitates displayed in figure 7 B.  The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63; lane 3, Syt VII (C/S)-YFP/FLAG-CD63.","Figure 1 C: Original data represented in the anti-actin western blot displayed in figure 1 C.  The Lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII (D/N)-YFP; lane 3, Syt VII (C/S)-YFP.","Original .lif files from Leica Acquisition Software represented in figure 8 B.  The left panel corresponds to cells treated with CD63 siRNA and the right panel corresponds to cells treated with control siRNA.   The channels correspond to the figure as follows: Channel 0, DAPI (Blue), Channel 1, anti-CD63 staining (RED).  Anti-Lamp1 staining (gray) corresponds to channel 2, which is not included in figure 8 B.","Figure 7 C: Original data represented in the Ponceau stain of the immunoprecipitates displayed in figure 7 C.  The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63(Y/A); lane 3, Syt VII (C/S)-YFP/FLAG-CD63(Y/A).<br /><br />","Figure 8 A: Original data represented in the anti-actin western blots displayed in figure 8 A.   The lanes correspond to the figure as follows: lane 1, Lipofectamine; lane 2, Control siRNA; lane 3, CD63 siRNA.","Figure 1 C: Original data represented in the anti-GFP western blot displayed in figure 1 C.  The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII (D/N)-YFP; lane 3, Syt VII (C/S)-YFP.","Original .lif files from Leica Acquisition Software represented in figure 8 B. The left panel corresponds to cells treated with CD63 siRNA and the right panel corresponds to cells treated with control siRNA. The channels correspond to the figure as follows: Channel 0, DAPI (Blue), Channel 1, anti-CD63 staining (RED). Anti-Lamp1 staining (gray) corresponds to channel 2, which is not included in figure 8 B.","Figure 7; B and C: Original data represented in the anti-GFP western blots of the whole cell lysates displayed in figure 7 B and C.  The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63; lane 3, Syt VII (C/S)-YFP/FLAG-CD63; lane 4, Syt VII-YFP; lane 5, SytVII-YFP/FLAG-CD63(Y/A); lane 6, SytVII (C/S)-YFP/FLAG-CD63(Y/A).","Figure 7; B and C: Original data represented in the anti-actin western blots of the whole cell lysates displayed in figure 7 B and C.  The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63; lane 3, Syt VII (C/S)-YFP/FLAG-CD63; lane 4, Syt VII-YFP; lane 5, SytVII-YFP/FLAG-CD63(Y/A); lane 6, SytVII (C/S)-YFP/FLAG-CD63(Y/A).","Figure 7; B and C: Original data represented in the anti-FLAG western blots displayed in figure 7 B and C.  The lanes correspond to: lane 1, WCL Syt VII-YFP; lane 2, WCL Syt VII-YFP/FLAG-CD63; lane 3, WCL Syt VII (C/S)-YFP/FLAG-CD63; lane 4, WCL Syt VII-YFP; lane 5, WCL SytVII-YFP/FLAG-CD63(Y/A); lane 6, WCL SytVII (C/S)-YFP/FLAG-CD63(Y/A); lane 7, IP Syt VII-YFP; lane 8, IP Syt VII-YFP/FLAG-CD63; lane 9, IP Syt VII (C/S)-YFP/FLAG-CD63; lane 10, IP Syt VII-YFP; lane 11, IP SytVII-YFP/FLAG-CD63(Y/A); lane 12, IP SytVII (C/S)-YFP/FLAG-CD63(Y/A). Whole Cell Lysate (WCL).  Immunoprecipitation (IP).","Figure 8 A: Original data represented in the anti-CD63 western blot displayed in figure 8 A.   The lanes correspond to the figure as follows: lane 1, Lipofectamine; lane 2, Control siRNA; lane 3, CD63 siRNA."],"repository":["bioimages"],"figure_sub":["Image 23738 (Figure 1 - C)","Figure 8 - A","Figure 8 - B","Image 23744 (Figure 7 - B)","Figure 7 - B","Figure 7 - C","Image 23773 (Figure 8 - B)","Figure 1 - C","Image 23742 (Figure 7 - B, C)","Image 23747 (Figure 8 - A)","Image 23739 (Figure 1 - C)","Image 23741 (Figure 7 - B, C)","Figure 7","Figure 7 - B, C","Figure 8","Image 23745 (Figure 7 - C)","Image 23740 (Figure 7 - B, C)","Figure 1","Image 23743 (Figure 7 - B, C)","Image 23774 (Figure 8 - B)","Image 23746 (Figure 8 - A)"],"pubmed_authors":["Andrew R. Flannery","Cecilia Czibener","Norma W. Andrews"],"additional_accession":[]},"is_claimable":false,"name":"Palmitoylation-dependent association with CD63 targets the Ca2+sensor synaptotagmin VII to lysosomes","description":null,"dates":{"release":"2010-11-01T11:20:07Z","modification":"2018-11-29T11:20:07Z","creation":"2018-11-29T11:20:07Z"},"accession":"S-JCBD-201003021","cross_references":{"doi":["10.1083/jcb.201003021"]}}