bioimagesUnknownAndrew R. FlanneryThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201003021JCB

Figure 7; B and C: Original data represented in the anti-GFP western blots of the immunoprecipitates displayed in figure 7 B and C. The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63; lane 3, Syt VII (C/S)-YFP/FLAG-CD63; lane 4, Syt VII-YFP; lane 5, SytVII-YFP/FLAG-CD63(Y/A); lane 6, SytVII (C/S)-YFP/FLAG-CD63(Y/A).

Figure 7B: Original data represented in the Ponceau stain of the immunoprecipitates displayed in figure 7 B. The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63; lane 3, Syt VII (C/S)-YFP/FLAG-CD63.

Figure 1 C: Original data represented in the anti-actin western blot displayed in figure 1 C. The Lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII (D/N)-YFP; lane 3, Syt VII (C/S)-YFP.

Original .lif files from Leica Acquisition Software represented in figure 8 B. The left panel corresponds to cells treated with CD63 siRNA and the right panel corresponds to cells treated with control siRNA. The channels correspond to the figure as follows: Channel 0, DAPI (Blue), Channel 1, anti-CD63 staining (RED). Anti-Lamp1 staining (gray) corresponds to channel 2, which is not included in figure 8 B.

Figure 7 C: Original data represented in the Ponceau stain of the immunoprecipitates displayed in figure 7 C. The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63(Y/A); lane 3, Syt VII (C/S)-YFP/FLAG-CD63(Y/A).<br /><br />

Figure 8 A: Original data represented in the anti-actin western blots displayed in figure 8 A. The lanes correspond to the figure as follows: lane 1, Lipofectamine; lane 2, Control siRNA; lane 3, CD63 siRNA.

Figure 1 C: Original data represented in the anti-GFP western blot displayed in figure 1 C. The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII (D/N)-YFP; lane 3, Syt VII (C/S)-YFP.

Original .lif files from Leica Acquisition Software represented in figure 8 B. The left panel corresponds to cells treated with CD63 siRNA and the right panel corresponds to cells treated with control siRNA. The channels correspond to the figure as follows: Channel 0, DAPI (Blue), Channel 1, anti-CD63 staining (RED). Anti-Lamp1 staining (gray) corresponds to channel 2, which is not included in figure 8 B.

Figure 7; B and C: Original data represented in the anti-GFP western blots of the whole cell lysates displayed in figure 7 B and C. The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63; lane 3, Syt VII (C/S)-YFP/FLAG-CD63; lane 4, Syt VII-YFP; lane 5, SytVII-YFP/FLAG-CD63(Y/A); lane 6, SytVII (C/S)-YFP/FLAG-CD63(Y/A).

Figure 7; B and C: Original data represented in the anti-actin western blots of the whole cell lysates displayed in figure 7 B and C. The lanes correspond to: lane 1, Syt VII-YFP; lane 2, Syt VII-YFP/FLAG-CD63; lane 3, Syt VII (C/S)-YFP/FLAG-CD63; lane 4, Syt VII-YFP; lane 5, SytVII-YFP/FLAG-CD63(Y/A); lane 6, SytVII (C/S)-YFP/FLAG-CD63(Y/A).

Figure 7; B and C: Original data represented in the anti-FLAG western blots displayed in figure 7 B and C. The lanes correspond to: lane 1, WCL Syt VII-YFP; lane 2, WCL Syt VII-YFP/FLAG-CD63; lane 3, WCL Syt VII (C/S)-YFP/FLAG-CD63; lane 4, WCL Syt VII-YFP; lane 5, WCL SytVII-YFP/FLAG-CD63(Y/A); lane 6, WCL SytVII (C/S)-YFP/FLAG-CD63(Y/A); lane 7, IP Syt VII-YFP; lane 8, IP Syt VII-YFP/FLAG-CD63; lane 9, IP Syt VII (C/S)-YFP/FLAG-CD63; lane 10, IP Syt VII-YFP; lane 11, IP SytVII-YFP/FLAG-CD63(Y/A); lane 12, IP SytVII (C/S)-YFP/FLAG-CD63(Y/A). Whole Cell Lysate (WCL). Immunoprecipitation (IP).

Figure 8 A: Original data represented in the anti-CD63 western blot displayed in figure 8 A. The lanes correspond to the figure as follows: lane 1, Lipofectamine; lane 2, Control siRNA; lane 3, CD63 siRNA.

bioimagesImage 23738 (Figure 1 - C)Figure 8 - AFigure 8 - BImage 23744 (Figure 7 - B)Figure 7 - BFigure 7 - CImage 23773 (Figure 8 - B)Figure 1 - CImage 23742 (Figure 7 - B, C)Image 23747 (Figure 8 - A)Image 23739 (Figure 1 - C)Image 23741 (Figure 7 - B, C)Figure 7Figure 7 - B, CFigure 8Image 23745 (Figure 7 - C)Image 23740 (Figure 7 - B, C)Figure 1Image 23743 (Figure 7 - B, C)Image 23774 (Figure 8 - B)Image 23746 (Figure 8 - A)Andrew R. FlanneryCecilia CzibenerNorma W. AndrewsfalsePalmitoylation-dependent association with CD63 targets the Ca2+sensor synaptotagmin VII to lysosomes2010-11-01T11:20:07Z2018-11-29T11:20:07Z2018-11-29T11:20:07ZS-JCBD-20100302110.1083/jcb.201003021