bioimagesUnknownDamien GarbettThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201004115JCB

Representative image stacks from the microvillar rescue assay. Control cells (siGL2) were not treated with siEBP50, and shows cells transfected to express the GFP-Nuc control plasmid. Cells were stained for ezrin (red) and actin (blue) and transfected cells identified as those expressing the GFP-tagged construct (green). Microvilli were visualized on the apical surface of cells by ezrin and phalloidin staining.<br />

Representative images of cells from the microvillar rescue assay. Cells were stained for ezrin (red) and actin (blue) and transfected cells identified as those expressing the GFP-tagged construct (green).

GFP-EBP50 wildtype and PKC phospho-mimetic (S162,339,340D) or –deficient (S162,339,340A) mutants were overexpressed in cells treated with PMA, stained for ezrin (red) and actin (blue) and their apical surface phenotype was scored.

Representative images from the microvillar rescue assay. Transfected cells were identified as expressing GFP-tagged constructs (green) and stained for ezrin (red) and actin (blue). Microvilli were visualized on the apical surface of cells by ezrin and phalloidin staining.

Representative images from the microvillar rescue assay. Cells were stained for ezrin (red) and actin (blue) and transfected cells identified as those expressing the GFP-tagged construct (green).

bioimagesImage 23940 (Figure 10 - B)Image 16592 (Figure 3 - B)Image 16607 (Figure 7 - B )Image 16598 (Figure 4 - B)Figure 10 - BImage 16602 (Figure 6 - B)Image 16599 (Figure 4 - B)Image 16601 (Figure 5 - B)Figure 7 - BFigure 3 - BImage 16596 (Figure 3 - B)Image 16593 (Figure 3 - B)Figure 4 - BSupFigure 3SupFigure 2Image 23941 (Figure 10 - B)Image 16600 (Figure 5 - B)Image 16610 (SupFigure 3 - B)Image 16608 (SupFigure 2 - B)Image 16609 (SupFigure 3 - B)Image 16594 (Figure 3 - B)Figure 5 - BImage 16606 (Figure 7 - B )SupFigure 3 - BFigure 5Figure 4Image 16597 (Figure 4 - B)Figure 7Image 23942 (Figure 10 - B)Figure 6Figure 10Image 16605 (Figure 7 - B )Image 16603 (Figure 6 - B)Image 16604 (Figure 6 - B)Figure 6 - BImage 23939 (Figure 10 - B)Figure 3SupFigure 2 - BImage 16595 (Figure 3 - B)Damien GarbettAnthony BretscherDavid P. LaLondefalseThe scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner2010-10-11T11:20:23Z2018-11-29T11:20:23Z2018-11-29T11:20:23ZS-JCBD-20100411510.1083/jcb.201004115