{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Manuel Breuer"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201005065"],"attach_to":["JCB"],"legend":["MI spindle (8h post NEBD) showing Hurp labeling (green), Crest (Red) and DNA labelling (blue, bottom image).","Control wt oocytes and Hurp -/-<br />oocytes were observed at NEBD +7h and labeled for MTOCs using anti-pericentrin (Red) and<br />DNA (Blue). An example of spindle collapse in a Hurp -<br />/- oocytes observed at NEBD+7h is depicted (Microtubules appear green, DNA in blue and MTOCs in<br />red).","Oocytes fixed at different stages during meiotic maturation and labeled with Hoechst for chromosomes (blue),  anti-Tubulin (green) and anti-Hurp (red).","Fixed wt oocytes (up) and Hurp-/-<br />oocytes (bottom) stained with DAPI to label chromosomes (blue), anti-Tubulin for<br />microtubules (green) and anti-Tpx2 (red).","Wt and Hurp -/-<br />oocytes stained with Hoechst to label chromosomes (blue), anti-Tubulin for microtubules<br />(green) and anti-Hurp (red).","Control wt oocytes and Hurp -/- oocytes were observed at NEBD +7h and labeled for MTOCs using anti-pericentrin (Red) and<br />DNA (Blue). An example of spindle collapse in a Hurp -<br />/- oocytes at NEBD+7h is depicted (Microtubules green, DNA in blue and MTOCs in red)."],"repository":["bioimages"],"figure_sub":["Figure 3 - D","Figure 1 - H","Image 17830 (Figure 1 - H)","Figure 5 - A","Image 17847 (SupFigure 1 - C)","Image 17839 (Figure 1 - A)","Figure 1 - A","Image 17829 (Figure 1 - A)","Image 17842 (Figure 3 - D)","Image 17827 (Figure 1 - A)","SupFigure 1 - C","Image 17840 (Figure 3 - D)","Figure 5","SupFigure 1","Image 17838 (Figure 1 - A)","Image 17848 (SupFigure 1 - C)","Image 17843 (Figure 3 - D)","Figure 1","Image 17844 (Figure 5 - A)","Figure 3","Image 17846 (Figure 5 - A)"],"pubmed_authors":["Manuel Breuer","David Pellman","Agnieszka Kolano","Chao-Chin Li","Marie-Hélène Verlhac","Mijung Kwon","Stéphane Brunet","Ting-Fen Tsai"],"additional_accession":[]},"is_claimable":false,"name":"HURP permits MTOC sorting for robust meiotic spindle bipolarity, similar to extra centrosome clustering in cancer cells","description":"<br />            In contrast to somatic cells, formation of acentriolar meiotic spindles relies on the organization of microtubules (MTs) and MT-organizing centers (MTOCs) into a stable bipolar structure. The underlying mechanisms are still unknown. We show that this process is impaired in<br />            <jats:italic>hepatoma up-regulated protein</jats:italic><br />            (<br />            <jats:italic>Hurp</jats:italic><br />            ) knockout mice, which are viable but female sterile, showing defective oocyte divisions. HURP accumulates on interpolar MTs in the vicinity of chromosomes via Kinesin-5 activity. By promoting MT stability in the spindle central domain, HURP allows efficient MTOC sorting into distinct poles, providing bipolarity establishment and maintenance. Our ","dates":{"release":"2010-12-20T11:20:32Z","modification":"2018-11-29T11:20:32Z","creation":"2018-11-29T11:20:32Z"},"accession":"S-JCBD-201005065","cross_references":{"doi":["10.1083/jcb.201005065"]}}