bioimagesUnknownManuel BreuerThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201005065JCB

MI spindle (8h post NEBD) showing Hurp labeling (green), Crest (Red) and DNA labelling (blue, bottom image).

Control wt oocytes and Hurp -/-<br />oocytes were observed at NEBD +7h and labeled for MTOCs using anti-pericentrin (Red) and<br />DNA (Blue). An example of spindle collapse in a Hurp -<br />/- oocytes observed at NEBD+7h is depicted (Microtubules appear green, DNA in blue and MTOCs in<br />red).

Oocytes fixed at different stages during meiotic maturation and labeled with Hoechst for chromosomes (blue), anti-Tubulin (green) and anti-Hurp (red).

Fixed wt oocytes (up) and Hurp-/-<br />oocytes (bottom) stained with DAPI to label chromosomes (blue), anti-Tubulin for<br />microtubules (green) and anti-Tpx2 (red).

Wt and Hurp -/-<br />oocytes stained with Hoechst to label chromosomes (blue), anti-Tubulin for microtubules<br />(green) and anti-Hurp (red).

Control wt oocytes and Hurp -/- oocytes were observed at NEBD +7h and labeled for MTOCs using anti-pericentrin (Red) and<br />DNA (Blue). An example of spindle collapse in a Hurp -<br />/- oocytes at NEBD+7h is depicted (Microtubules green, DNA in blue and MTOCs in red).

bioimagesFigure 3 - DFigure 1 - HImage 17830 (Figure 1 - H)Figure 5 - AImage 17847 (SupFigure 1 - C)Image 17839 (Figure 1 - A)Figure 1 - AImage 17829 (Figure 1 - A)Image 17842 (Figure 3 - D)Image 17827 (Figure 1 - A)SupFigure 1 - CImage 17840 (Figure 3 - D)Figure 5SupFigure 1Image 17838 (Figure 1 - A)Image 17848 (SupFigure 1 - C)Image 17843 (Figure 3 - D)Figure 1Image 17844 (Figure 5 - A)Figure 3Image 17846 (Figure 5 - A)Manuel BreuerDavid PellmanAgnieszka KolanoChao-Chin LiMarie-Hélène VerlhacMijung KwonStéphane BrunetTing-Fen TsaifalseHURP permits MTOC sorting for robust meiotic spindle bipolarity, similar to extra centrosome clustering in cancer cells<br /> In contrast to somatic cells, formation of acentriolar meiotic spindles relies on the organization of microtubules (MTs) and MT-organizing centers (MTOCs) into a stable bipolar structure. The underlying mechanisms are still unknown. We show that this process is impaired in<br /> <jats:italic>hepatoma up-regulated protein</jats:italic><br /> (<br /> <jats:italic>Hurp</jats:italic><br /> ) knockout mice, which are viable but female sterile, showing defective oocyte divisions. HURP accumulates on interpolar MTs in the vicinity of chromosomes via Kinesin-5 activity. By promoting MT stability in the spindle central domain, HURP allows efficient MTOC sorting into distinct poles, providing bipolarity establishment and maintenance. Our 2010-12-20T11:20:32Z2018-11-29T11:20:32Z2018-11-29T11:20:32ZS-JCBD-20100506510.1083/jcb.201005065