bioimagesUnknownVenizelos PapayannopoulosThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201006052JCB

NE, PR3, and MPO localization during NET formation.<br /><br />PMA-activated neutrophils (60 min) were fixed and immunolabeled for NE (red) and MPO (green). DNA was stained with DRAQ5 (blue). <br /><br />MPO associates with DNA before cell lysis but later than NE.

Figure 6.<br /><br />NE knockout mice fail to form NETs. <br /><br />(A) Representative fluorescence images of the lungs of WT (i) and NE knockout (ii) mice infected with K. pneumoniae, and stained with antibodies against MPO (green) and against a DNA/histone complex (red). The lungs of WT mice (i) contain decondensed web-like chromatin structures that stain for MPO (arrow). In contrast, in the lungs of NE knockout mice, all neutrophils appear naive, with condensed nuclei and granular MPO staining.

NE, PR3, and MPO localization during NET formation.<br /><br />PMA-activated neutrophils (60 min) were fixed and immunolabeled for NE (red) and MPO (green). DNA was stained with DRAQ5 (blue).<br /><br />MPO associates with DNA before cell lysis but later than NE.

NE, PR3, and MPO localization during NET formation. Naive and PMA-activated neutrophils in the presence or absence of NEi, fixed at the indicated time points and immunolabeled for NE (red) and MPO (green). DNA was stained with DRAQ5 (blue). <br /><br />NEi prevents NE and MPO translocation to the nucleus, and chromatin decondensation.

bioimagesImage 27029 (Figure 5 - C)Image 27032 (Figure 5 - C)Image 27056 (Figure 6 - Ai)Image 27040 (Figure 5 - C)Figure 5 - B upperImage 27038 (Figure 5 - C)Image 27055 (Figure 6 - Aii)Image 27036 (Figure 5 - C)Image 27051 (Figure 6 - Ai)Image 27042 (Figure 5 - C)Image 27045 (Figure 5 - B upper)Figure 6 - AiiImage 27033 (Figure 5 - C)Image 27041 (Figure 5 - C)Image 27048 (Figure 5 - B lower)Image 27037 (Figure 5 - C)Image 27046 (Figure 5 - B upper)Image 27043 (Figure 5 - C)Image 27047 (Figure 5 - B lower)Image 27030 (Figure 5 - C)Figure 5 - B lowerImage 27034 (Figure 5 - C)Figure 5 - CFigure 5Image 27031 (Figure 5 - C)Image 27044 (Figure 5 - B upper)Figure 6Image 27049 (Figure 5 - B lower)Figure 6 - AiImage 27035 (Figure 5 - C)Image 27054 (Figure 6 - Aii)Image 27039 (Figure 5 - C)Arturo ZychlinskyVenizelos PapayannopoulosAbdul HakkimKathleen D. MetzlerfalseNeutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps2010-10-25T11:20:37Z2018-11-29T11:20:37Z2018-11-29T11:20:37ZS-JCBD-20100605210.1083/jcb.201006052