bioimagesUnknownKatharina RzeczkowskiThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201006089JCB

HEK2931L-1R cells were transfected to express GFP-DCP1a and HA-JNK K55R and were analyzed for GFP-DCP1a (green) and immunostained for JNK (red).

HEK293IL-1R cells were stimulated with anisomycin (10µg/ml) for 120min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with IL-1 alpha(10ng/ml) for 120min. Localization of DCP1a (green) and Edc4 (red) (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were left untreated. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stably transfected with GFP-DCP1a S315A. Co-localization of endogenous JNK (red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with anisomycin (10µg/ml) for 60min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stably transfected with GFP-DCP1a S315A. Co-localization of endogenous Edc4 (red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were stimulated with anisomycin (10µg/ml) for 60min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence. <br />

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with anisomycin (10µg/ml) for 60min. Localization of DCP1a (green) and Edc4 (red) (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with sorbitol (0.5M) for 120min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with sorbitol (0.5M) for 60min. Localization of DCP1a (green) and Edc4 (red)(red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with anisomycin (10µg/ml) for 60min. Localization of DCP1a (green) and Edc4 (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with IL-1alpha (10ng/ml) for 120min. Localization of DCP1a (green) and Edc4 (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with anisomycin (10µg/ml) for 60min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with anisomycin (10µg/ml) for 120min. Localization of DCP1a (green) and Edc4 (red) was analyzed by double-immunofluorescence.

HEK2931L-1R cells were transfected to express GFP-DCP1a and HA-TAK1-TAB1 and HA-JNK K55R and were analyzed for GFP-DCP1a (green) and immunostained for JNK (red).

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with IL-1alpha (10ng/ml) for 60min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with IL-1alpha (10ng/ml) for 120min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with anisomycin (10µg/ml) for 120min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stably transfected with GFP-DCP1a S315A. Co-localization of endogenous Xrn1 ( red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were stably transfected with wild type GFP-DCP1a(wt). Co-localization of endogenous Edc4 (red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were left untreated. Double-immunofluorescence omitting 1st antibodies was performed as a negative control for Xrn1/P-DCP1a staining.

HEK293IL-1R cells were left untreated. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with IL-1alpha (10ng/ml) for 60min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with IL-1 alpha(10ng/ml) for 120min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence. <br />

HEK293IL-1R cells were stimulated with sorbitol (0.5M) for 120min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK2931L-1R cells were transfected to express GFP-DCP1a and MEKK1delta-BFP were analyzed for GFP-DCP1a (green) and immunostainined for JNK (red).

HEK2931L-1R cells were transfected to express GFP-DCP1a and HA-JNK and were analyzed for GFP-DCP1a (green) and immunostained for JNK (red).

HEK293IL-1R cells were stably transfected with a C-terminal deletion mutant of GFP-DCP1a(delta515-582). Co-localization of endogenous JNK (red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were stimulated with IL-1alpha (10ng/ml) for 120min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with sorbitol (0.5M) for 60min. Localization of DCP1a (green) and Edc4 (red) was analyzed by double-immunofluorescence.

GFP-DCP1a, negative control for Edc4 staining.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with IL-1 alpha(10ng/ml) for 120min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK2931L-1R cells were transfected to express GFP-DCP1a and MEKK1delta-BFP and were analyzed for GFP-DCP1a (green) and immunostained for JNK (red).

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with anisomycin (10µg/ml) for 120min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stably transfected with wild type GFP-DCP1a(wt). Co-localization of endogenous Xrn1 (red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were left untreated. Double-immunofluorescence omitting 1st antibodies was performed as a negative control for DCP1a/Edc4 staining.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with sorbitol (0.5M) for 120min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with IL-1alpha (10ng/ml) for 60min. Localization of DCP1a (green) and Edc4 (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with IL-1alpha (10ng/ml) for 60min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with sorbitol (0.5M) for 60min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stably transfected with GFP-DCP1a S315D. Co-localization of endogenous JNK (red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were stably transfected with GFP-DCP1a S315D. Co-localization of endogenous Xrn1 ( red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were stably transfected with wild type GFP-DCP1a(wt). Co-localization of endogenous JNK (red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with sorbitol (0.5M) for 120min. Localization of DCP1a (green) and Edc4 (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with anisomycin (10µg/ml) for 60min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stably transfected with a C-terminal deletion mutant of GFP-DCP1a(delta515-582). Co-localization of endogenous Edc4 (red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were stably transfected with GFP-DCP1a S315D. Co-localization of endogenous Edc4 (red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with sorbitol (0.5M) for 60min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were left untreated. Localization of DCP1a (green) and Edc4 (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stably transfected with a C-terminal deletion mutant of GFP-DCP1a(delta515-582). Co-localization of endogenous Xrn1 ( red) with stably expressed GFP-DCP1a (green) was analyzed by fluorescence microscopy.

HEK293IL-1R cells were stimulated with sorbitol (0.5M) for 120min. Localization of DCP1a (green) and Edc4 (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with IL-1alpha (10ng/ml) for 60min. Localization of DCP1a (green) and Edc4 (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were stimulated with sorbitol (0.5M) for 60min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

GFP-DCP1a, negative control for JNK staining.

HEK2931L-1R cells were transfected to express GFP-DCP1a and HA-TAK1-TAB1 and HA-JNK and were analyzed for GFP-DCP1a (green) and immunostained for JNK (red).

HEK293IL-1R cells were stimulated with IL-1alpha (10ng/ml) for 60min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with anisomycin (10µg/ml) for 120min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

GFP-DCP1a, negative control for Xrn1 staining.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with sorbitol (0.5M) for 60min. Localization of Xrn1 (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were pretreated for 30min with SP600125 (20µM) and then stimulated with anisomycin (10µg/ml) for 120min. Localization of DCP1a (green) and Edc4 (red) (red) was analyzed by double-immunofluorescence.

HEK2931L-1R cells were transfected to express GFP-DCP1a and HA-TAK1-TAB1 and were analyzed for GFP-DCP1a (green) and immunostained for JNK (red).

HEK293IL-1R cells were stimulated with sorbitol (0.5M) for 120min. Localization of DCP1a (green) and P(S315)-DCP1a (red) was analyzed by double-immunofluorescence.

HEK293IL-1R cells were left untreated. Double-immunofluorescence omitting 1st antibodies was performed as a negative control for DCP1a/P-DCP1a staining.

bioimagesImage 32665 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32657 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Figure 1AImage 32647 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32663 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32653 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32662 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32664 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32666 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32667 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32668 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32393 (Figure 1A - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Image 32388 (Figure 1A - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Image 32660 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32669 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32394 (Figure 1A - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Image 32644 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32656 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32439 (Figure 6C - 1- colabeling for GFP-DCP1a (green; direct fluorescence), Xrn1 (red; immunofluorescence), and Hoechst 33342 (blue))Image 32401 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32402 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32648 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32652 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Figure 6C - 2 - colabeling for GFP-DCP1a (green; direct fluorescence), Edc4 (red; immunofluorescence) and Hoechst 33342 (blue)Figure 6CImage 32437 (Figure 6C - 1- colabeling for GFP-DCP1a (green; direct fluorescence), Xrn1 (red; immunofluorescence), and Hoechst 33342 (blue))Image 32441 (Figure 6C - 1- colabeling for GFP-DCP1a (green; direct fluorescence), Xrn1 (red; immunofluorescence), and Hoechst 33342 (blue))Image 32403 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue)Image 32447 (Figure 6C - 3 - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Image 32449 (Figure 6C - 3 - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Figure 1A - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue)Image 32400 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32645 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32672 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32649 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32655 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32396 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32408 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32390 (Figure 1A - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Image 32651 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32406 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32446 (Figure 6C - 2 - colabeling for GFP-DCP1a (green; direct fluorescence), Edc4 (red; immunofluorescence) and Hoechst 33342 (blue))Image 32399 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32670 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32671 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32395 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32407 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32391 (Figure 1A - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Image 32442 (Figure 6C - 2 - colabeling for GFP-DCP1a (green; direct fluorescence), Edc4 (red; immunofluorescence) and Hoechst 33342 (blue))Image 32404 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32445 (Figure 6C - 2 - colabeling for GFP-DCP1a (green; direct fluorescence), Edc4 (red; immunofluorescence) and Hoechst 33342 (blue))Image 32398 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32646 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue)Image 32405 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32444 (Figure 6C - 2 - colabeling for GFP-DCP1a (green; direct fluorescence), Edc4 (red; immunofluorescence) and Hoechst 33342 (blue))Image 32450 (Figure 6C - 3 - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Image 32451 (Figure 6C - 3 - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Image 32397 (Figure 4B - 1 - costaining for DCP1a (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32443 (Figure 6C - 2 - colabeling for GFP-DCP1a (green; direct fluorescence), Edc4 (red; immunofluorescence) and Hoechst 33342 (blue))Image 32658 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Image 32659 (Figure 4B - 3 - costaining for DCP1a (green), Edc4 (red), and Hoechst 33342 (blue))Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue)Image 32438 (Figure 6C - 1- colabeling for GFP-DCP1a (green; direct fluorescence), Xrn1 (red; immunofluorescence), and Hoechst 33342 (blue))Image 32654 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32392 (Figure 1A - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Image 32389 (Figure 1A - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Figure 4BImage 32440 (Figure 6C - 1- colabeling for GFP-DCP1a (green; direct fluorescence), Xrn1 (red; immunofluorescence), and Hoechst 33342 (blue))Figure 6C - 1- colabeling for GFP-DCP1a (green; direct fluorescence), Xrn1 (red; immunofluorescence), and Hoechst 33342 (blue)Image 32650 (Figure 4B - 2 - costaining for Xrn1 (green), P(S315)-DCP1a (red), and Hoechst 33342 (blue))Image 32448 (Figure 6C - 3 - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue))Figure 6C - 3 - colabeling for GFP-DCP1a (green; direct fluorescence), JNK (red; immunofluorescence), and Hoechst 33342 (blue)Helmut MüllerKnut BeuerleinHelmut HoltmannHeike SchneiderDaniela Kettner-BuhrowMichael KrachtKatharina RzeczkowskiOliver Dittrich-Breiholzfalsec-Jun N-terminal kinase phosphorylates DCP1a to control formation of P bodies2011-08-22T11:20:40Z2018-11-29T11:20:40Z2018-11-29T11:20:40ZS-JCBD-20100608910.1083/jcb.201006089