{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["David Cohen"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201007002"],"attach_to":["JCB"],"legend":["cell spreading and compaction in IRSp53-KD-MDCK monolayers  in <br />- dox upon plating on collagen I ;Bright field time lapse series (40x, 1frame/10min), corresponding to Movie M10, right panel","Brightfield and mRFP timelapse of freshly plated Par1b cells cultured in -dox (1frame/15min) corresponding to movie M6, right panel","actin dynamics in T23-MDCK cells expressing Par1b-GFP and mRFP actin; time lapse series of GFP, mRFP and Bright field (63 x, 1 frame/3min), corresponding to Movie 5, lower panel","spreading and actin dynamics in IRSp53KD-MDCK cells cultured on collagen-I in +dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (63x,1frame/3sec), corresponding to movie M7, left panel","paxillin dynamics in IRSp53-KD -dox cells  expressing  paxilin-mCherry, mCherry and Bright Field time lapse   (63x, 1 frame/ 15 min), corresponding to movie M 8, right panel","x-y series through confluent IRSp53 cell cultures in -dox, 24h after Ca-switch; phalloidin=red, E-cadherin=green, ZO1=blue","Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 24h after Ca-switch in IRSp53-KD cells in +dox<br />","Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 3h after Ca-switch in IRSp53-KD cells in –dox<br />","x-y series through confluent Par1b cell cultures in +dox, 24h after Ca-switch; phalloidin=red, E-cadherin=green, ZO1=blue","paxillin dynamics in IRSp53-KD +dox cells  expressing  paxilin-mCherry, mCherry and Bright Field time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 8, left panel","Brightfield  time lapse (1 frame/15min) of Par1b cells cultured in +dox 30’ to 6 h after plating on collagen-I, corresponding to movie M6, left panel","time lapse depicting paxillin dynamics in IRSp53-KD -dox cells  expressing  paxilin-mCherry (63x, 1 frame/ 15 min), corresponding to movie M 8, right panel","spreading and actin dynamics in Par1b-KD-MDCK cells cultured in  +dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (63x, 1frame/3sec), corresponding to movie M2, left panel<br /><br />","Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 8h after Ca-switch in IRSp53-KD cells in +dox","actin dynamics in T23-MDCK cells expressing GFP and mRFP actin; time lapse series of GFP, mRFP and Bright field  (63 x, 1 frame/3min), corresponding to Movie 5, upper panel","spreading and actin dynamics in Par1b-MDCK cells cultured in –dox  and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (40x, 1frame/1min) corresponding to movie M1, right panel","paxillin dynamics in T23-MDCK cells expressing Par1b-GFP and paxilin-mCherry: mCherry and GFP fluorescence time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 3, right panel","paxillin dynamics in T23-MDCK cells expressing  paxilin-mCherry: mCherry and GFP fluorescence time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 4, right panel","spreading and actin dynamics in IRSp53KD-MDCK cells cultured on collagen-I in -dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (63x,1frame/3sec), corresponding to movie M7, right panel","spreading and actin dynamics in Par1b-KD-MDCK cells cultured in either –dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (63x, 1frame/3sec), corresponding to movie M2, right panel<br /><br />","paxillin dynamics in Par1b-KD cells in -dox expressing  paxilin-mCherry: mCherry   fluorescence and Bright field time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 4, right panel","Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling  8h after Ca-switch in IRSp53-KD cells in –dox<br />","paxillin dynamics in T23-MDCK cells expressing GFP and paxilin-mCherry: mCherry and GFP fluorescence time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 3, left panel","time lapse depicting paxillin dynamics in IRSp53-KD +dox cells  expressing  paxilin-mCherry (63x, 1 frame/ 15 min), corresponding to movie M 8, left panel","Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling  8h after Ca-switch in IRSp53-KD cells in +dox","x-y series through confluent IRSp53 cell cultures in +dox, 24h after Ca-switch; phalloidin=red, E-cadherin=green, ZO1=blue","x-y series through confluent Par1b cell cultures in -dox, 24h after Ca-switch; phalloidin=red, E-cadherin=green, ZO1=blue","spreading and actin dynamics in Par1b-MDCK cells cultured in  +dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (40x , 1frame/1min) corresponding to movie M1, left panel","paxillin dynamics in Par1b-KDcells cultured in +dox expressing  paxilin-mCherry: mCherry and GFP fluorescence time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 4, left panel","Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 24h after Ca-switch in IRSp53-KD cells in –dox<br />","Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 3h after Ca-switch in IRSp53-KD cells in +dox <br />","paxillin dynamics in Par1b-KDcells cultured in +dox expressing  paxillin-mCherry: mCherry fluorescence and Bright field time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 4, left panel","time lapse depicting paxillin dynamics in IRSp53-WT  cells  expressing  paxilin-mCherry and Par1b-GFP (63x, 1 frame/ 15 min), corresponding to movie M 9, left panel","cell spreading and compaction in IRSp53-KD-MDCK monolayers  in + dox upon plating on collagen I ;Bright field time lapse series (40x, 1frame/10min), corresponding to Movie M 10, left panel","time lapse depicting paxillin dynamics in IRSp53-AA  cells  expressing  paxilin-mCherry and Par1b-GFP (63x, 1 frame/ 15 min), corresponding to movie M 9, right panel"],"repository":["bioimages"],"figure_sub":["Image 22336 (Figure 1 - A)","Image 22342 (Figure 1 - D)","Image 22347 (Figure 1 - D)","Supplemental Figure 4","Image 22380 (Figure 4 - D)","Image 22363 (Figure 1 - D)","Supplemental Figure 4 - A+C","Image 22368 (Figure 1 - D)","Image 22356 (Figure 1 - D)","Image 22381 (Figure 4 - F)","Image 22354 (Figure 1 - D)","Image 22401 (Figure 5 - C)","Image 22384 (Figure 4 - F)","Image 22389 (Figure 4 - F)","Image 22396 (Figure 5 - C)","Image 22351 (Figure 1 - D)","Image 22406 (Figure 5 - C)","Image 22359 (Figure 1 - D)","Image 22378 (Figure 4 - C)","Image 22393 (Figure 4 - F)","Image 22379 (Figure 4 - D)","Image 22412 (Supplemental Figure 4 - A+C)","Figure 1 - F","Image 22349 (Figure 1 - D)","Image 22353 (Figure 1 - D)","Image 22405 (Figure 5 - C)","Image 22366 (Figure 1 - D)","Image 22387 (Figure 4 - F)","Figure 1 - A","Image 22352 (Figure 1 - D)","Image 22371 (Figure 1 - E)","Image 22388 (Figure 4 - F)","Image 22415 (Supplemental Figure 4 - A+C)","Image 22390 (Figure 4 - F)","Figure 1 - E","Figure 1 - D","Figure 1 - C","Image 22375 (Figure 4 - B)","Image 22377 (Figure 4 - C)","Image 22373 (Figure 1 - F)","Image 22348 (Figure 1 - D)","Image 22398 (Figure 5 - C)","Image 22404 (Figure 5 - C)","Image 22365 (Figure 1 - D)","Image 22386 (Figure 4 - F)","Image 22403 (Figure 5 - C)","Image 22399 (Figure 5 - C)","Image 22409 (Supplemental Figure 4 - A+C)","Image 22370 (Figure 1 - D)","Image 22391 (Figure 4 - F)","Image 22372 (Figure 1 - E)","Image 22374 (Figure 1 - F)","Image 22416 (Supplemental Figure 4 - A+C)","Image 22385 (Figure 4 - F)","Image 22397 (Figure 5 - C)","Image 22355 (Figure 1 - D)","Image 22350 (Figure 1 - D)","Image 22364 (Figure 1 - D)","Image 22402 (Figure 5 - C)","Image 22341 (Figure 1 - D)","Image 22343 (Figure 1 - D)","Image 22394 (Figure 4 - F)","Image 22367 (Figure 1 - D)","Figure 4 - B","Image 22392 (Figure 4 - F)","Image 22410 (Supplemental Figure 4 - A+C)","Image 22335 (Figure 1 - A)","Image 22413 (Supplemental Figure 4 - A+C)","Image 22340 (Figure 1 - C)","Image 22346 (Figure 1 - D)","Figure 4 - F","Figure 4 - C","Image 22376 (Figure 4 - B)","Figure 4 - D","Image 22400 (Figure 5 - C)","Image 22361 (Figure 1 - D)","Image 22395 (Figure 5 - C)","Image 22345 (Figure 1 - D)","Image 22339 (Figure 1 - C)","Image 22383 (Figure 4 - F)","Image 22360 (Figure 1 - D)","Figure 5 - C","Image 22358 (Figure 1 - D)","Figure 5","Image 22414 (Supplemental Figure 4 - A+C)","Figure 4","Image 22362 (Figure 1 - D)","Image 22337 (Figure 1 - C)","Image 22369 (Figure 1 - D)","Image 22338 (Figure 1 - C)","Image 22357 (Figure 1 - D)","Image 22411 (Supplemental Figure 4 - A+C)","Image 22382 (Figure 4 - F)","Figure 1","Image 22344 (Figure 1 - D)","Image 22417 (Supplemental Figure 4 - A+C)"],"pubmed_authors":["David Cohen","Francisco Lázaro-Diéguez","Dawn Fernandez","Anne Müsch"],"additional_accession":[]},"is_claimable":false,"name":"The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell–ECM signaling","description":null,"dates":{"release":"2011-01-31T11:20:47Z","modification":"2018-11-29T11:20:47Z","creation":"2018-11-29T11:20:47Z"},"accession":"S-JCBD-201007002","cross_references":{"doi":["10.1083/jcb.201007002"]}}