bioimagesUnknownDavid CohenThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201007002JCBcell spreading and compaction in IRSp53-KD-MDCK monolayers in <br />- dox upon plating on collagen I ;Bright field time lapse series (40x, 1frame/10min), corresponding to Movie M10, right panelBrightfield and mRFP timelapse of freshly plated Par1b cells cultured in -dox (1frame/15min) corresponding to movie M6, right panelactin dynamics in T23-MDCK cells expressing Par1b-GFP and mRFP actin; time lapse series of GFP, mRFP and Bright field (63 x, 1 frame/3min), corresponding to Movie 5, lower panelspreading and actin dynamics in IRSp53KD-MDCK cells cultured on collagen-I in +dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (63x,1frame/3sec), corresponding to movie M7, left panelpaxillin dynamics in IRSp53-KD -dox cells expressing paxilin-mCherry, mCherry and Bright Field time lapse (63x, 1 frame/ 15 min), corresponding to movie M 8, right panelx-y series through confluent IRSp53 cell cultures in -dox, 24h after Ca-switch; phalloidin=red, E-cadherin=green, ZO1=bluePhalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 24h after Ca-switch in IRSp53-KD cells in +dox<br />Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 3h after Ca-switch in IRSp53-KD cells in –dox<br />x-y series through confluent Par1b cell cultures in +dox, 24h after Ca-switch; phalloidin=red, E-cadherin=green, ZO1=bluepaxillin dynamics in IRSp53-KD +dox cells expressing paxilin-mCherry, mCherry and Bright Field time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 8, left panelBrightfield time lapse (1 frame/15min) of Par1b cells cultured in +dox 30’ to 6 h after plating on collagen-I, corresponding to movie M6, left paneltime lapse depicting paxillin dynamics in IRSp53-KD -dox cells expressing paxilin-mCherry (63x, 1 frame/ 15 min), corresponding to movie M 8, right panelspreading and actin dynamics in Par1b-KD-MDCK cells cultured in +dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (63x, 1frame/3sec), corresponding to movie M2, left panel<br /><br />Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 8h after Ca-switch in IRSp53-KD cells in +doxactin dynamics in T23-MDCK cells expressing GFP and mRFP actin; time lapse series of GFP, mRFP and Bright field (63 x, 1 frame/3min), corresponding to Movie 5, upper panelspreading and actin dynamics in Par1b-MDCK cells cultured in –dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (40x, 1frame/1min) corresponding to movie M1, right panelpaxillin dynamics in T23-MDCK cells expressing Par1b-GFP and paxilin-mCherry: mCherry and GFP fluorescence time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 3, right panelpaxillin dynamics in T23-MDCK cells expressing paxilin-mCherry: mCherry and GFP fluorescence time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 4, right panelspreading and actin dynamics in IRSp53KD-MDCK cells cultured on collagen-I in -dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (63x,1frame/3sec), corresponding to movie M7, right panelspreading and actin dynamics in Par1b-KD-MDCK cells cultured in either –dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (63x, 1frame/3sec), corresponding to movie M2, right panel<br /><br />paxillin dynamics in Par1b-KD cells in -dox expressing paxilin-mCherry: mCherry fluorescence and Bright field time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 4, right panelPhalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 8h after Ca-switch in IRSp53-KD cells in –dox<br />paxillin dynamics in T23-MDCK cells expressing GFP and paxilin-mCherry: mCherry and GFP fluorescence time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 3, left paneltime lapse depicting paxillin dynamics in IRSp53-KD +dox cells expressing paxilin-mCherry (63x, 1 frame/ 15 min), corresponding to movie M 8, left panelPhalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 8h after Ca-switch in IRSp53-KD cells in +doxx-y series through confluent IRSp53 cell cultures in +dox, 24h after Ca-switch; phalloidin=red, E-cadherin=green, ZO1=bluex-y series through confluent Par1b cell cultures in -dox, 24h after Ca-switch; phalloidin=red, E-cadherin=green, ZO1=bluespreading and actin dynamics in Par1b-MDCK cells cultured in +dox and expressing mRFP-actin. Bright field and mRFP-fluorescence time lapse series (40x , 1frame/1min) corresponding to movie M1, left panelpaxillin dynamics in Par1b-KDcells cultured in +dox expressing paxilin-mCherry: mCherry and GFP fluorescence time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 4, left panelPhalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 24h after Ca-switch in IRSp53-KD cells in –dox<br />Phalloidin (red), E-cadherin (green) and ZO-1 (blue) labeling 3h after Ca-switch in IRSp53-KD cells in +dox <br />paxillin dynamics in Par1b-KDcells cultured in +dox expressing paxillin-mCherry: mCherry fluorescence and Bright field time lapse series (63x, 1 frame/ 15 min), corresponding to movie M 4, left paneltime lapse depicting paxillin dynamics in IRSp53-WT cells expressing paxilin-mCherry and Par1b-GFP (63x, 1 frame/ 15 min), corresponding to movie M 9, left panelcell spreading and compaction in IRSp53-KD-MDCK monolayers in + dox upon plating on collagen I ;Bright field time lapse series (40x, 1frame/10min), corresponding to Movie M 10, left paneltime lapse depicting paxillin dynamics in IRSp53-AA cells expressing paxilin-mCherry and Par1b-GFP (63x, 1 frame/ 15 min), corresponding to movie M 9, right panelbioimagesImage 22336 (Figure 1 - A)Image 22342 (Figure 1 - D)Image 22347 (Figure 1 - D)Supplemental Figure 4Image 22380 (Figure 4 - D)Image 22363 (Figure 1 - D)Supplemental Figure 4 - A+CImage 22368 (Figure 1 - D)Image 22356 (Figure 1 - D)Image 22381 (Figure 4 - F)Image 22354 (Figure 1 - D)Image 22401 (Figure 5 - C)Image 22384 (Figure 4 - F)Image 22389 (Figure 4 - F)Image 22396 (Figure 5 - C)Image 22351 (Figure 1 - D)Image 22406 (Figure 5 - C)Image 22359 (Figure 1 - D)Image 22378 (Figure 4 - C)Image 22393 (Figure 4 - F)Image 22379 (Figure 4 - D)Image 22412 (Supplemental Figure 4 - A+C)Figure 1 - FImage 22349 (Figure 1 - D)Image 22353 (Figure 1 - D)Image 22405 (Figure 5 - C)Image 22366 (Figure 1 - D)Image 22387 (Figure 4 - F)Figure 1 - AImage 22352 (Figure 1 - D)Image 22371 (Figure 1 - E)Image 22388 (Figure 4 - F)Image 22415 (Supplemental Figure 4 - A+C)Image 22390 (Figure 4 - F)Figure 1 - EFigure 1 - DFigure 1 - CImage 22375 (Figure 4 - B)Image 22377 (Figure 4 - C)Image 22373 (Figure 1 - F)Image 22348 (Figure 1 - D)Image 22398 (Figure 5 - C)Image 22404 (Figure 5 - C)Image 22365 (Figure 1 - D)Image 22386 (Figure 4 - F)Image 22403 (Figure 5 - C)Image 22399 (Figure 5 - C)Image 22409 (Supplemental Figure 4 - A+C)Image 22370 (Figure 1 - D)Image 22391 (Figure 4 - F)Image 22372 (Figure 1 - E)Image 22374 (Figure 1 - F)Image 22416 (Supplemental Figure 4 - A+C)Image 22385 (Figure 4 - F)Image 22397 (Figure 5 - C)Image 22355 (Figure 1 - D)Image 22350 (Figure 1 - D)Image 22364 (Figure 1 - D)Image 22402 (Figure 5 - C)Image 22341 (Figure 1 - D)Image 22343 (Figure 1 - D)Image 22394 (Figure 4 - F)Image 22367 (Figure 1 - D)Figure 4 - BImage 22392 (Figure 4 - F)Image 22410 (Supplemental Figure 4 - A+C)Image 22335 (Figure 1 - A)Image 22413 (Supplemental Figure 4 - A+C)Image 22340 (Figure 1 - C)Image 22346 (Figure 1 - D)Figure 4 - FFigure 4 - CImage 22376 (Figure 4 - B)Figure 4 - DImage 22400 (Figure 5 - C)Image 22361 (Figure 1 - D)Image 22395 (Figure 5 - C)Image 22345 (Figure 1 - D)Image 22339 (Figure 1 - C)Image 22383 (Figure 4 - F)Image 22360 (Figure 1 - D)Figure 5 - CImage 22358 (Figure 1 - D)Figure 5Image 22414 (Supplemental Figure 4 - A+C)Figure 4Image 22362 (Figure 1 - D)Image 22337 (Figure 1 - C)Image 22369 (Figure 1 - D)Image 22338 (Figure 1 - C)Image 22357 (Figure 1 - D)Image 22411 (Supplemental Figure 4 - A+C)Image 22382 (Figure 4 - F)Figure 1Image 22344 (Figure 1 - D)Image 22417 (Supplemental Figure 4 - A+C)David CohenFrancisco Lázaro-DiéguezDawn FernandezAnne MüschfalseThe serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell–ECM signaling2011-01-31T11:20:47Z2018-11-29T11:20:47Z2018-11-29T11:20:47ZS-JCBD-20100700210.1083/jcb.201007002