{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Mauricio Valerio-Santiago"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201007044"],"attach_to":["JCB"],"legend":["3HA-Bfa1 (red) localization in wild-type metaphase cells. Tubulin (green) and DAPI (blue) are also shown.","Localization of eGFP-Tem1-CAAX (green) in GAL-UPL-TEM1 cells growing in glucose. DIC is also shown.","3HA-Bfa1 (red) localization in wild type anaphase cells. Tubulin (green) and DAPI (blue) are also shown.","Localization of eGFP-Cnm67-Tem1 (green) in nud1-2 cells with a misaligned spindle. DAPI (blue) and DIC are also shown.","Localization of eGFP-Tem1-CAAX (green) in GAL-UPL-TEM1 anaphase cells lacking Bfa1. DIC is also shown.","Localization of 3HA-Cdc15 (red) in metaphase cells expressing Cnm67-Tem1. Tubulin (green) and DAPI (blue) are also shown.","Localization of Tem1-eGFP (green) in a wild-type cell in metaphase. DAPI (blue) and DIC are also shown.","Localization of Tem1-eGFP (green) in wild type anaphase cells. DAPI (blue) and DIC are also shown.","Localization of Tem1-eGFP (green) in a cdc15-2 mutant growing at 37ºC. DAPI (blue) and DIC are also shown.","3HA-Cdc14 (red) localization in anaphase. The protein is released throughout the whole cell. Tubulin (green) and DAPI (blue) are also shown.","eGFP-Cnm67-Tem1 (green) localization in GAL-UPL1-TEM1 anaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.","3HA-Bfa1 (red) localization in metaphase cells expressing Cnm67-Tem1. Tubulin (green) and DAPI (blue) are also shown.","Localization of eGFP-Tem1 (green) in GAL-UPL-TEM1 anaphase cells lacking Bfa1. DIC is also shown.","Localization of Tem1-eGFP (green) in a bfa1 mutant in metaphase. DAPI (blue) and DIC are also shown.","3HA-Bfa1 (red) localization in anaphase cells expressing Cnm67-Tem1. Tubulin (green) and DAPI (blue) are also shown.","Localization of Tem1-eGFP (green) and Bfa1-mCherry (red) in metaphase cells. DAPI (blue) and DIC are also shown.","Localization of Tem1-eGFP (green) in a cdc15-2 bfa1-null mutant growing at 37ºC. DAPI (blue) and DIC are also shown.","eGFP-Cnm67-Tem1 (green) localization in GAL-UPL1-TEM1 metaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.","3HA-Cdc14 (red) localization in metaphase. The protein is sequestered in the nucleolus. Tubulin (green) and DAPI (blue) are also shown.","DIC image of cells expressing KIN4 from the GAL1-10 promoter and growing in medium with 2% glucose.","Localization of eGFP-Cnm67-Tem1 (green) in nud1-2 cells with an aligned spindle. DAPI (blue) and DIC are also shown.","Localization of Tem1-eGFP (green) and Bfa1-mCherry (red) in anaphase cells. DAPI (blue) and DIC are also shown.","eGFP-Tem1 (green) localization in GAL-UPL1-TEM1 anaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.","3HA-Cdc15 (red) localization in wild-type metaphase cells. Tubulin (green) and DAPI (blue) are also shown.","eGFP-Bfa1-Tem1 (green) localization in GAL-UPL1-TEM1 anaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.","DIC image of cells expressing KIN4 from the GAL1-10 promoter and CNM67-TEM1 from the TEM1 promoter growing in medium with 2% glucose.","3HA-Cdc15 (red) localization in metaphase cells expressing Bfa1-Tem1. Tubulin (green) and DAPI (blue) are also shown.","DIC image of cells expressing KIN4 from the GAL1-10 promoter and growing in medium with 2% galactose / 2% raffinose.","3HA-Bfa1 (red) localization in dyn1 mutant expressing the Cnm67-Tem1 chimera. Tubulin (green), DAPI (blue) and DIC are also shown.","eGFP-Tem1 (green) localization in GAL-UPL1-TEM1 metaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.","DIC image of cells expressing KIN4 from the GAL1-10 promoter and CNM67-TEM1 from the TEM1 promoter growing in medium with 2% galactose / 2% raffinose.","Localization of Tem1-eGFP (green) in anaphase cells lacking Bfa1. DAPI (blue) and DIC are also shown.","Localization of eGFP-Cnm67-Tem1 (green) in nud1-2 rebudded cells. DAPI (blue) and DIC are also shown.","eGFP-Bfa1-Tem1 (green) localization in GAL-UPL1-TEM1 metaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.","3HA-Cdc14 (red) localization in early anaphase. The protein is partially released throughout the nucleus. Tubulin (green) and DAPI (blue) are also shown."],"repository":["bioimages"],"figure_sub":["Image 30586 (Figure 1 - B)","Image 30545 (Figure S4 - B)","Image 30583 (Figure 1 - A)","Figure 7 - A","Image 30549 (Figure S5 - D)","Figure 7 - F","Image 30539 (Figure 7 - A)","Figure S5","Figure S4","Figure S3","Image 30627 (Figure 5 - C)","Figure S3 - A","Image 30535 (Figure 6 - A)","Image 30546 (Figure S4 - D)","Image 30524 (Figure 3 - D)","Image 30542 (Figure 7 - F)","Image 30600 (Figure 4 - C)","Image 30628 (Figure 5 - C)","Image 30534 (Figure 6 - A)","Figure S4 - D","Figure S4 - B","Image 30536 (Figure 6 - B)","Figure 1 - A","Image 30544 (Figure S4 - B)","Image 30592 (Figure 7 - F)","Image 30602 (Figure 4 - D)","Figure 1 - B","Image 30629 (Figure 5 - C)","Image 30601 (Figure 4 - D)","Figure S5 - D","Figure 3 - D","Image 30525 (Figure 3 - D)","Image 30543 (Figure S3 - A)","Figure 3 - C","Image 30585 (Figure 1 - B)","Image 30591 (Figure 7 - A)","Image 30522 (Figure 3 - C)","Image 30537 (Figure 6 - B)","Figure 4 - C","Figure 4 - D","Image 30523 (Figure 3 - D)","Image 30584 (Figure 1 - B)","Image 30581 (Figure 1 - A)","Image 30599 (Figure 4 - C)","Image 30521 (Figure 3 - C)","Figure 5 - C","Figure 5","Figure 4","Figure 7","Figure 6","Image 30547 (Figure S4 - D)","Image 30548 (Figure S5 - D)","Image 30582 (Figure 1 - A)","Image 30626 (Figure 5 - C)","Figure 6 - A","Image 30520 (Figure 3 - C)","Figure 6 - B","Figure 1","Image 30540 (Figure 7 - F)","Figure 3"],"pubmed_authors":["Fernando Monje-Casas","Mauricio Valerio-Santiago"],"additional_accession":[]},"is_claimable":false,"name":"Tem1 localization to the spindle pole bodies is essential for mitotic exit and impairs spindle checkpoint function","description":null,"dates":{"release":"2011-02-14T11:20:53Z","modification":"2018-11-29T11:20:53Z","creation":"2018-11-29T11:20:53Z"},"accession":"S-JCBD-201007044","cross_references":{"doi":["10.1083/jcb.201007044"]}}