bioimagesUnknownMauricio Valerio-SantiagoThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201007044JCB

3HA-Bfa1 (red) localization in wild-type metaphase cells. Tubulin (green) and DAPI (blue) are also shown.

Localization of eGFP-Tem1-CAAX (green) in GAL-UPL-TEM1 cells growing in glucose. DIC is also shown.

3HA-Bfa1 (red) localization in wild type anaphase cells. Tubulin (green) and DAPI (blue) are also shown.

Localization of eGFP-Cnm67-Tem1 (green) in nud1-2 cells with a misaligned spindle. DAPI (blue) and DIC are also shown.

Localization of eGFP-Tem1-CAAX (green) in GAL-UPL-TEM1 anaphase cells lacking Bfa1. DIC is also shown.

Localization of 3HA-Cdc15 (red) in metaphase cells expressing Cnm67-Tem1. Tubulin (green) and DAPI (blue) are also shown.

Localization of Tem1-eGFP (green) in a wild-type cell in metaphase. DAPI (blue) and DIC are also shown.

Localization of Tem1-eGFP (green) in wild type anaphase cells. DAPI (blue) and DIC are also shown.

Localization of Tem1-eGFP (green) in a cdc15-2 mutant growing at 37ºC. DAPI (blue) and DIC are also shown.

3HA-Cdc14 (red) localization in anaphase. The protein is released throughout the whole cell. Tubulin (green) and DAPI (blue) are also shown.

eGFP-Cnm67-Tem1 (green) localization in GAL-UPL1-TEM1 anaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.

3HA-Bfa1 (red) localization in metaphase cells expressing Cnm67-Tem1. Tubulin (green) and DAPI (blue) are also shown.

Localization of eGFP-Tem1 (green) in GAL-UPL-TEM1 anaphase cells lacking Bfa1. DIC is also shown.

Localization of Tem1-eGFP (green) in a bfa1 mutant in metaphase. DAPI (blue) and DIC are also shown.

3HA-Bfa1 (red) localization in anaphase cells expressing Cnm67-Tem1. Tubulin (green) and DAPI (blue) are also shown.

Localization of Tem1-eGFP (green) and Bfa1-mCherry (red) in metaphase cells. DAPI (blue) and DIC are also shown.

Localization of Tem1-eGFP (green) in a cdc15-2 bfa1-null mutant growing at 37ºC. DAPI (blue) and DIC are also shown.

eGFP-Cnm67-Tem1 (green) localization in GAL-UPL1-TEM1 metaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.

3HA-Cdc14 (red) localization in metaphase. The protein is sequestered in the nucleolus. Tubulin (green) and DAPI (blue) are also shown.

DIC image of cells expressing KIN4 from the GAL1-10 promoter and growing in medium with 2% glucose.

Localization of eGFP-Cnm67-Tem1 (green) in nud1-2 cells with an aligned spindle. DAPI (blue) and DIC are also shown.

Localization of Tem1-eGFP (green) and Bfa1-mCherry (red) in anaphase cells. DAPI (blue) and DIC are also shown.

eGFP-Tem1 (green) localization in GAL-UPL1-TEM1 anaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.

3HA-Cdc15 (red) localization in wild-type metaphase cells. Tubulin (green) and DAPI (blue) are also shown.

eGFP-Bfa1-Tem1 (green) localization in GAL-UPL1-TEM1 anaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.

DIC image of cells expressing KIN4 from the GAL1-10 promoter and CNM67-TEM1 from the TEM1 promoter growing in medium with 2% glucose.

3HA-Cdc15 (red) localization in metaphase cells expressing Bfa1-Tem1. Tubulin (green) and DAPI (blue) are also shown.

DIC image of cells expressing KIN4 from the GAL1-10 promoter and growing in medium with 2% galactose / 2% raffinose.

3HA-Bfa1 (red) localization in dyn1 mutant expressing the Cnm67-Tem1 chimera. Tubulin (green), DAPI (blue) and DIC are also shown.

eGFP-Tem1 (green) localization in GAL-UPL1-TEM1 metaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.

DIC image of cells expressing KIN4 from the GAL1-10 promoter and CNM67-TEM1 from the TEM1 promoter growing in medium with 2% galactose / 2% raffinose.

Localization of Tem1-eGFP (green) in anaphase cells lacking Bfa1. DAPI (blue) and DIC are also shown.

Localization of eGFP-Cnm67-Tem1 (green) in nud1-2 rebudded cells. DAPI (blue) and DIC are also shown.

eGFP-Bfa1-Tem1 (green) localization in GAL-UPL1-TEM1 metaphase cells growing in 2% glucose. DAPI (blue) and DIC are also shown.

3HA-Cdc14 (red) localization in early anaphase. The protein is partially released throughout the nucleus. Tubulin (green) and DAPI (blue) are also shown.

bioimagesImage 30586 (Figure 1 - B)Image 30545 (Figure S4 - B)Image 30583 (Figure 1 - A)Figure 7 - AImage 30549 (Figure S5 - D)Figure 7 - FImage 30539 (Figure 7 - A)Figure S5Figure S4Figure S3Image 30627 (Figure 5 - C)Figure S3 - AImage 30535 (Figure 6 - A)Image 30546 (Figure S4 - D)Image 30524 (Figure 3 - D)Image 30542 (Figure 7 - F)Image 30600 (Figure 4 - C)Image 30628 (Figure 5 - C)Image 30534 (Figure 6 - A)Figure S4 - DFigure S4 - BImage 30536 (Figure 6 - B)Figure 1 - AImage 30544 (Figure S4 - B)Image 30592 (Figure 7 - F)Image 30602 (Figure 4 - D)Figure 1 - BImage 30629 (Figure 5 - C)Image 30601 (Figure 4 - D)Figure S5 - DFigure 3 - DImage 30525 (Figure 3 - D)Image 30543 (Figure S3 - A)Figure 3 - CImage 30585 (Figure 1 - B)Image 30591 (Figure 7 - A)Image 30522 (Figure 3 - C)Image 30537 (Figure 6 - B)Figure 4 - CFigure 4 - DImage 30523 (Figure 3 - D)Image 30584 (Figure 1 - B)Image 30581 (Figure 1 - A)Image 30599 (Figure 4 - C)Image 30521 (Figure 3 - C)Figure 5 - CFigure 5Figure 4Figure 7Figure 6Image 30547 (Figure S4 - D)Image 30548 (Figure S5 - D)Image 30582 (Figure 1 - A)Image 30626 (Figure 5 - C)Figure 6 - AImage 30520 (Figure 3 - C)Figure 6 - BFigure 1Image 30540 (Figure 7 - F)Figure 3Fernando Monje-CasasMauricio Valerio-SantiagofalseTem1 localization to the spindle pole bodies is essential for mitotic exit and impairs spindle checkpoint function2011-02-14T11:20:53Z2018-11-29T11:20:53Z2018-11-29T11:20:53ZS-JCBD-20100704410.1083/jcb.201007044