{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Myriam Ruault"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201008007"],"attach_to":["JCB"],"legend":["Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 8h in galactose containing medium.","Representative fluorescence Z-stack image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 8h in galactose containing medium.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 3h in galactose containing medium.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 6h in galactose containing medium.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a wild-type strain<br />","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ strain grown in galactose medium","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ yku70∆ GAL1-SIR3 strain grown in galactose","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain owild-type strain","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP and Sir3 tagged with mCherry in a strain overexpressing Sir3-mCherry under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of a sir2∆ strain overexpressing Sir3-GFP under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 4h30 in galactose containing medium.","Live cell Z-stack fluorescence image of a strain overexpressing Sir3-GFP under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown in raffinose containing medium.","Live cell Z-stack fluorescence image of a sir2∆ sir4∆ strain overexpressing Sir3-A2Q-GFP under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain co-overexpressing Sir3 and Sir4 under the control of the GAL1 promoter","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 6h in galactose containing medium.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ GAL1-SIR3 strain grown in glucose","Live cell Z-stack fluorescence image of CBF2-GFP in a strain repressing SIR3 (GAL1p-SIR3 in glucose medium).","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 1h30 in galactose containing medium.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir2∆ sir4∆ strain overexpressing Sir3-A2Q under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir2 under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a wild-type strain","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown in raffinose containing medium.","Representative fluorescence Z-stack image of the telomere-associated protein Rap1 tagged with GFP in a WT strain.","Live cell Z-stack fluorescence image of a strain expressing the telomere-associated protein Rap1 tagged with GFP and the nucleolar protein Sik1 tagged with mRFP. Green channel.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP and Sir3 tagged with mCherry in a strain overexpressing Sir3 under the control of the GAL1 promoter.<br />","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3-A2Q under the control of the GAL1 promoter.","Immuno-FISH experiment performed on a strain overexpressing Sir3-A2Q. Sir3 is detected using a polyclonal anti-Sir3 antibody and a secondary rabbit antibody coupled to FITC. Z-stack fluorescence image acquired with a deltavision microscope, FITC channel.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing SIR3 under the control of the GAL1 promoter","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 1h30 in galactose containing medium.","Immuno-FISH experiment performed on a strain overexpressing Sir3. Z-stack fluorescence image acquired with a deltavision microscope, FITC channel.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 45 minutes in galactose containing medium.<br />","Immuno-FISH experiment performed on a strain overexpressing Sir3-A2Q. Z-stack fluorescence image acquired with a deltavision microscope, RD-TR-PE channel.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing the N-terminal part of SIR3 under the control of the ADH promoter from a 2 micron plasmid","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 3h in galactose containing medium.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a mps3∆75-150 strain overexpressing SIR3 under the control of the GAL1 promoter","Immuno-FISH experiment performed in a wild-type strain. Z-stack fluorescence image.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir2∆ strain overexpressing Sir3-A2Q under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of a sir2∆ strain overexpressing Sir3-A2Q-GFP under the control of the GAL1 promoter.<br />","Live cell Z-stack fluorescence image of CBF2-GFP in a strain overexpressing SIR3 (GAL1p-SIR3 in galactose medium).","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a mps3∆75-150 strain","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 4h30 in galactose containing medium.","Live cell Z-stack fluorescence image of a strain overexpressing Sir3 and expressing the telomere-associated protein Rap1 tagged with GFP as well as the nucleolar protein Sik1 tagged with mRFP. Green channel.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain co-overexpressing Sir2, Sir3 and Sir4 under the control of the GAL1 promoter","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir2∆ strain overexpressing Sir3 under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of a strain expressing the telomere-associated protein Rap1 tagged with GFP and the nucleolar protein Sik1 tagged with mRFP. Red channel.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a esc1∆ strain overexpressing SIR3 under the control of the GAL1 promoter","Live cell Z-stack fluorescence image of a strain expressing Sir4 tagged with GFP.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir3∆ strain carrying a centromeric vector expressing SIR3 under the control of its own promoter","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoter","Immuno-FISH experiment performed on a strain overexpressing Sir3-A2Q. Rap1-GFP signal was detected using a primary anti-GFP antibody and a secondary antibody coupled to FITC. Z-stack fluorescence image acquired with a Deltavision microscope, FITC channel.","Immuno-FISH experiment performed on a strain overexpressing Sir3. Z-stack fluorescence image acquired with a deltavision microscope, RD-TR-PE channel.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3 under the control of the GALS promoter.","Live cell Z-stack fluorescence image of a strain overexpressing Sir3-A2Q-GFP under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of a strain overexpressing Sir3 and expressing the telomere-associated protein Rap1 tagged with GFP as well as the nucleolar protein Sik1 tagged with mRFP. Red channel.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir1∆ strain overexpressing SIR3 under the control of the GAL1 promoter","Live cell Z-stack fluorescence image of a strain expressing Sir3 tagged with GFP.","Live cell Z-stack fluorescence image of sir4-tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of a sir2∆ sir4∆ strain overexpressing Sir3-GFP under the control of the GAL1 promoter.<br />","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir3∆ strain carrying a centromeric vector expressing sir3-A2Q under the control of its own promoter<br />","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir1∆ strain","Live cell Z-stack fluorescence image of SPC42-mCherry in a strain repressing SIR3 (GAL1p-SIR3 in glucose medium).","Immuno-FISH experiment performed on a strain overexpressing Sir3-A2Q. Z-stack fluorescence image acquired with a Deltavision micrsocope, RD-TR-PE channel.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in the wild-type strain.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 45 minutes in galactose containing medium.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain co-overexpressing Sir2 and Sir3 under the control of the GAL1 promoter","Live cell Z-stack fluorescence image of a rap1-17 strain overexpressing Sir3-A2Q-GFP under the control of the GAL1 promoter.<br />","Immuno-FISH experiment performed in a strain overexpressing Sir3. Z-stack fluorescence image.","Live cell Z-stack fluorescence image of sir2-tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of a strain expressing Sir2 tagged with GFP.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a esc1∆ strain","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir4 under the control of the GAL1 promoter.","Representative fluorescence Z-stack image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3-A2Q under the control of the GAL1 promoter.","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir3∆ strain carrying an empty vector.","Live cell Z-stack fluorescence image of SPC42-mCherry in a strain overexpressing SIR3 (GAL1p-SIR3 in galactose medium).","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ yku70∆ GAL1-SIR3 strain grown in glucose","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing SIR3 under the control of the ADH promoter from a 2 micron plasmid","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ strain grown in glucose medium","Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ GAL1-SIR3 strain grown in galactose"],"repository":["bioimages"],"figure_sub":["Image 29737 (Supplemental Figure 3 - A)","Image 29636 (Supplemental Figure 1 - D)","Image 29723 (Supplemental Figure 2 - None)","Image 29760 (Supplemental Figure 4 - F)","Image 29523 (Figure 6 - A)","Image 29746 (Supplemental Figure 4 - D)","Image 29755 (Supplemental Figure 4 - F)","Image 29608 (Figure 1 - B)","Image 29629 (Figure 6 - E)","Image 29726 (Supplemental Figure 2 - None)","Image 29742 (Supplemental Figure 4 - B)","Image 29766 (Supplemental Figure 1 - C)","Image 29517 (Figure 5 - B)","Image 29745 (Supplemental Figure 4 - D)","Image 29734 (Supplemental Figure 2 - None)","Image 29618 (Figure 2 - A)","Image 29529 (Figure 6 - C)","Image 29765 (Supplemental Figure 1 - C)","Image 29729 (Supplemental Figure 2 - None)","Figure 1 - B","Image 29617 (Figure 2 - A)","Image 29763 (Supplemental Figure 5 - A)","Image 29528 (Figure 6 - C)","Image 29733 (Supplemental Figure 2 - None)","Image 29756 (Supplemental Figure 4 - F)","Image 29743 (Supplemental Figure 4 - B)","Image 29519 (Figure 5 - B)","Image 29530 (Figure 6 - C)","Image 29609 (Figure 1 - B)","Image 29767 (Supplemental Figure 1 - C)","Image 29762 (Supplemental Figure 5 - A)","Image 29611 (Figure 1 - B)","Figure 3 - B","Image 29741 (Supplemental Figure 4 - A)","Image 29731 (Supplemental Figure 2 - None)","Image 29615 (Figure 2 - A)","Image 29627 (Figure 6 - D)","Image 29526 (Figure 6 - C)","Image 29730 (Supplemental Figure 2 - None)","Image 29522 (Figure 6 - A)","Supplemental Figure 3 - A","Image 29750 (Supplemental Figure 4 - D)","Image 29749 (Supplemental Figure 4 - D)","Image 29613 (Figure 2 - A)","Figure 5 - B","Figure 5 - C","Image 29621 (Figure 2 - C)","Image 29623 (Figure 2 - C)","Figure 5","Supplemental Figure 2 - None","Image 29757 (Supplemental Figure 4 - F)","Figure 6","Image 29751 (Supplemental Figure 4 - E)","Image 29625 (Figure 3 - B)","Image 29637 (Supplemental Figure 1 - D)","Figure 1","Supplemental Figure 5 - A","Figure 3","Figure 2","Image 29527 (Figure 6 - C)","Image 29732 (Supplemental Figure 2 - None)","Supplemental Figure 4","Supplemental Figure 5","Supplemental Figure 1 - C","Supplemental Figure 2","Image 29616 (Figure 2 - A)","Supplemental Figure 3","Image 29520 (Figure 5 - C)","Supplemental Figure 1","Supplemental Figure 1 - D","Image 29630 (Figure 6 - E)","Image 29628 (Figure 6 - D)","Image 29753 (Supplemental Figure 4 - E)","Image 29735 (Supplemental Figure 2 - None)","Image 29518 (Figure 5 - B)","Image 29739 (Supplemental Figure 3 - A)","Image 29740 (Supplemental Figure 4 - A)","Image 29761 (Supplemental Figure 5 - A)","Image 29752 (Supplemental Figure 4 - E)","Image 29525 (Figure 6 - A)","Image 29619 (Figure 2 - A)","Image 29638 (Supplemental Figure 1 - D)","Image 29758 (Supplemental Figure 4 - F)","Image 29744 (Supplemental Figure 4 - B)","Image 29748 (Supplemental Figure 4 - D)","Figure 2 - C","Image 29524 (Figure 6 - A)","Figure 2 - A","Image 29768 (Supplemental Figure 1 - C)","Image 29728 (Supplemental Figure 2 - None)","Image 29747 (Supplemental Figure 4 - D)","Image 29754 (Supplemental Figure 4 - E)","Image 29738 (Supplemental Figure 3 - A)","Image 29624 (Figure 3 - B)","Image 29727 (Supplemental Figure 2 - None)","Image 29612 (Figure 2 - A)","Image 29620 (Figure 2 - C)","Image 29521 (Figure 5 - C)","Image 29736 (Supplemental Figure 3 - A)","Image 29722 (Supplemental Figure 2 - None)","Image 29759 (Supplemental Figure 4 - F)","Image 29635 (Supplemental Figure 1 - D)","Image 29764 (Supplemental Figure 5 - A)","Image 29626 (Figure 3 - B)","Image 29725 (Supplemental Figure 2 - None)","Supplemental Figure 4 - A","Supplemental Figure 4 - B","Supplemental Figure 4 - D","Supplemental Figure 4 - E","Supplemental Figure 4 - F","Image 29614 (Figure 2 - A)","Figure 6 - C","Figure 6 - D","Figure 6 - A","Image 29724 (Supplemental Figure 2 - None)","Figure 6 - E","Image 29610 (Figure 1 - B)","Image 29622 (Figure 2 - C)"],"pubmed_authors":["Myriam Ruault","Isabelle Loïodice","Arnaud De Meyer","Angela Taddei"],"additional_accession":[]},"is_claimable":false,"name":"Clustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast","description":null,"dates":{"release":"2011-02-07T11:20:59Z","modification":"2018-11-29T11:20:59Z","creation":"2018-11-29T11:20:59Z"},"accession":"S-JCBD-201008007","cross_references":{"doi":["10.1083/jcb.201008007"]}}