bioimagesUnknownMyriam RuaultThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201008007JCBLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 8h in galactose containing medium.Representative fluorescence Z-stack image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 8h in galactose containing medium.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 3h in galactose containing medium.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 6h in galactose containing medium.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a wild-type strain Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ strain grown in galactose mediumLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ yku70∆ GAL1-SIR3 strain grown in galactoseLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain owild-type strainLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP and Sir3 tagged with mCherry in a strain overexpressing Sir3-mCherry under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of a sir2∆ strain overexpressing Sir3-GFP under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 4h30 in galactose containing medium.Live cell Z-stack fluorescence image of a strain overexpressing Sir3-GFP under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown in raffinose containing medium.Live cell Z-stack fluorescence image of a sir2∆ sir4∆ strain overexpressing Sir3-A2Q-GFP under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain co-overexpressing Sir3 and Sir4 under the control of the GAL1 promoterLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 6h in galactose containing medium.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ GAL1-SIR3 strain grown in glucoseLive cell Z-stack fluorescence image of CBF2-GFP in a strain repressing SIR3 (GAL1p-SIR3 in glucose medium).Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 1h30 in galactose containing medium.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir2∆ sir4∆ strain overexpressing Sir3-A2Q under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir2 under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a wild-type strainLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown in raffinose containing medium.Representative fluorescence Z-stack image of the telomere-associated protein Rap1 tagged with GFP in a WT strain.Live cell Z-stack fluorescence image of a strain expressing the telomere-associated protein Rap1 tagged with GFP and the nucleolar protein Sik1 tagged with mRFP. Green channel.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP and Sir3 tagged with mCherry in a strain overexpressing Sir3 under the control of the GAL1 promoter. Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3-A2Q under the control of the GAL1 promoter.Immuno-FISH experiment performed on a strain overexpressing Sir3-A2Q. Sir3 is detected using a polyclonal anti-Sir3 antibody and a secondary rabbit antibody coupled to FITC. Z-stack fluorescence image acquired with a deltavision microscope, FITC channel.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing SIR3 under the control of the GAL1 promoterLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 1h30 in galactose containing medium.Immuno-FISH experiment performed on a strain overexpressing Sir3. Z-stack fluorescence image acquired with a deltavision microscope, FITC channel.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 45 minutes in galactose containing medium. Immuno-FISH experiment performed on a strain overexpressing Sir3-A2Q. Z-stack fluorescence image acquired with a deltavision microscope, RD-TR-PE channel.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing the N-terminal part of SIR3 under the control of the ADH promoter from a 2 micron plasmidLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 3h in galactose containing medium.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a mps3∆75-150 strain overexpressing SIR3 under the control of the GAL1 promoterImmuno-FISH experiment performed in a wild-type strain. Z-stack fluorescence image.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir2∆ strain overexpressing Sir3-A2Q under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of a sir2∆ strain overexpressing Sir3-A2Q-GFP under the control of the GAL1 promoter. Live cell Z-stack fluorescence image of CBF2-GFP in a strain overexpressing SIR3 (GAL1p-SIR3 in galactose medium).Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a mps3∆75-150 strainLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a wild type strain grown 4h30 in galactose containing medium.Live cell Z-stack fluorescence image of a strain overexpressing Sir3 and expressing the telomere-associated protein Rap1 tagged with GFP as well as the nucleolar protein Sik1 tagged with mRFP. Green channel.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain co-overexpressing Sir2, Sir3 and Sir4 under the control of the GAL1 promoterLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir2∆ strain overexpressing Sir3 under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of a strain expressing the telomere-associated protein Rap1 tagged with GFP and the nucleolar protein Sik1 tagged with mRFP. Red channel.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a esc1∆ strain overexpressing SIR3 under the control of the GAL1 promoterLive cell Z-stack fluorescence image of a strain expressing Sir4 tagged with GFP.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir3∆ strain carrying a centromeric vector expressing SIR3 under the control of its own promoterLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoterImmuno-FISH experiment performed on a strain overexpressing Sir3-A2Q. Rap1-GFP signal was detected using a primary anti-GFP antibody and a secondary antibody coupled to FITC. Z-stack fluorescence image acquired with a Deltavision microscope, FITC channel.Immuno-FISH experiment performed on a strain overexpressing Sir3. Z-stack fluorescence image acquired with a deltavision microscope, RD-TR-PE channel.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3 under the control of the GALS promoter.Live cell Z-stack fluorescence image of a strain overexpressing Sir3-A2Q-GFP under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of a strain overexpressing Sir3 and expressing the telomere-associated protein Rap1 tagged with GFP as well as the nucleolar protein Sik1 tagged with mRFP. Red channel.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir1∆ strain overexpressing SIR3 under the control of the GAL1 promoterLive cell Z-stack fluorescence image of a strain expressing Sir3 tagged with GFP.Live cell Z-stack fluorescence image of sir4-tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of a sir2∆ sir4∆ strain overexpressing Sir3-GFP under the control of the GAL1 promoter. Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir3∆ strain carrying a centromeric vector expressing sir3-A2Q under the control of its own promoter Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir1∆ strainLive cell Z-stack fluorescence image of SPC42-mCherry in a strain repressing SIR3 (GAL1p-SIR3 in glucose medium).Immuno-FISH experiment performed on a strain overexpressing Sir3-A2Q. Z-stack fluorescence image acquired with a Deltavision micrsocope, RD-TR-PE channel.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in the wild-type strain.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP of a GAL1-SIR3 strain grown for 45 minutes in galactose containing medium.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain co-overexpressing Sir2 and Sir3 under the control of the GAL1 promoterLive cell Z-stack fluorescence image of a rap1-17 strain overexpressing Sir3-A2Q-GFP under the control of the GAL1 promoter. Immuno-FISH experiment performed in a strain overexpressing Sir3. Z-stack fluorescence image.Live cell Z-stack fluorescence image of sir2-tagged with GFP in a strain overexpressing Sir3 under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of a strain expressing Sir2 tagged with GFP.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a esc1∆ strainLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir4 under the control of the GAL1 promoter.Representative fluorescence Z-stack image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing Sir3-A2Q under the control of the GAL1 promoter.Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a sir3∆ strain carrying an empty vector.Live cell Z-stack fluorescence image of SPC42-mCherry in a strain overexpressing SIR3 (GAL1p-SIR3 in galactose medium).Live cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ yku70∆ GAL1-SIR3 strain grown in glucoseLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a strain overexpressing SIR3 under the control of the ADH promoter from a 2 micron plasmidLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ strain grown in glucose mediumLive cell Z-stack fluorescence image of the telomere-associated protein Rap1 tagged with GFP in a rif1∆ GAL1-SIR3 strain grown in galactosebioimagesImage 29737 (Supplemental Figure 3 - A)Image 29636 (Supplemental Figure 1 - D)Image 29723 (Supplemental Figure 2 - None)Image 29760 (Supplemental Figure 4 - F)Image 29523 (Figure 6 - A)Image 29746 (Supplemental Figure 4 - D)Image 29755 (Supplemental Figure 4 - F)Image 29608 (Figure 1 - B)Image 29629 (Figure 6 - E)Image 29726 (Supplemental Figure 2 - None)Image 29742 (Supplemental Figure 4 - B)Image 29766 (Supplemental Figure 1 - C)Image 29517 (Figure 5 - B)Image 29745 (Supplemental Figure 4 - D)Image 29734 (Supplemental Figure 2 - None)Image 29618 (Figure 2 - A)Image 29529 (Figure 6 - C)Image 29765 (Supplemental Figure 1 - C)Image 29729 (Supplemental Figure 2 - None)Figure 1 - BImage 29617 (Figure 2 - A)Image 29763 (Supplemental Figure 5 - A)Image 29528 (Figure 6 - C)Image 29733 (Supplemental Figure 2 - None)Image 29756 (Supplemental Figure 4 - F)Image 29743 (Supplemental Figure 4 - B)Image 29519 (Figure 5 - B)Image 29530 (Figure 6 - C)Image 29609 (Figure 1 - B)Image 29767 (Supplemental Figure 1 - C)Image 29762 (Supplemental Figure 5 - A)Image 29611 (Figure 1 - B)Figure 3 - BImage 29741 (Supplemental Figure 4 - A)Image 29731 (Supplemental Figure 2 - None)Image 29615 (Figure 2 - A)Image 29627 (Figure 6 - D)Image 29526 (Figure 6 - C)Image 29730 (Supplemental Figure 2 - None)Image 29522 (Figure 6 - A)Supplemental Figure 3 - AImage 29750 (Supplemental Figure 4 - D)Image 29749 (Supplemental Figure 4 - D)Image 29613 (Figure 2 - A)Figure 5 - BFigure 5 - CImage 29621 (Figure 2 - C)Image 29623 (Figure 2 - C)Figure 5Supplemental Figure 2 - NoneImage 29757 (Supplemental Figure 4 - F)Figure 6Image 29751 (Supplemental Figure 4 - E)Image 29625 (Figure 3 - B)Image 29637 (Supplemental Figure 1 - D)Figure 1Supplemental Figure 5 - AFigure 3Figure 2Image 29527 (Figure 6 - C)Image 29732 (Supplemental Figure 2 - None)Supplemental Figure 4Supplemental Figure 5Supplemental Figure 1 - CSupplemental Figure 2Image 29616 (Figure 2 - A)Supplemental Figure 3Image 29520 (Figure 5 - C)Supplemental Figure 1Supplemental Figure 1 - DImage 29630 (Figure 6 - E)Image 29628 (Figure 6 - D)Image 29753 (Supplemental Figure 4 - E)Image 29735 (Supplemental Figure 2 - None)Image 29518 (Figure 5 - B)Image 29739 (Supplemental Figure 3 - A)Image 29740 (Supplemental Figure 4 - A)Image 29761 (Supplemental Figure 5 - A)Image 29752 (Supplemental Figure 4 - E)Image 29525 (Figure 6 - A)Image 29619 (Figure 2 - A)Image 29638 (Supplemental Figure 1 - D)Image 29758 (Supplemental Figure 4 - F)Image 29744 (Supplemental Figure 4 - B)Image 29748 (Supplemental Figure 4 - D)Figure 2 - CImage 29524 (Figure 6 - A)Figure 2 - AImage 29768 (Supplemental Figure 1 - C)Image 29728 (Supplemental Figure 2 - None)Image 29747 (Supplemental Figure 4 - D)Image 29754 (Supplemental Figure 4 - E)Image 29738 (Supplemental Figure 3 - A)Image 29624 (Figure 3 - B)Image 29727 (Supplemental Figure 2 - None)Image 29612 (Figure 2 - A)Image 29620 (Figure 2 - C)Image 29521 (Figure 5 - C)Image 29736 (Supplemental Figure 3 - A)Image 29722 (Supplemental Figure 2 - None)Image 29759 (Supplemental Figure 4 - F)Image 29635 (Supplemental Figure 1 - D)Image 29764 (Supplemental Figure 5 - A)Image 29626 (Figure 3 - B)Image 29725 (Supplemental Figure 2 - None)Supplemental Figure 4 - ASupplemental Figure 4 - BSupplemental Figure 4 - DSupplemental Figure 4 - ESupplemental Figure 4 - FImage 29614 (Figure 2 - A)Figure 6 - CFigure 6 - DFigure 6 - AImage 29724 (Supplemental Figure 2 - None)Figure 6 - EImage 29610 (Figure 1 - B)Image 29622 (Figure 2 - C)Myriam RuaultIsabelle LoïodiceArnaud De MeyerAngela TaddeifalseClustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast2011-02-07T11:20:59Z2018-11-29T11:20:59Z2018-11-29T11:20:59ZS-JCBD-20100800710.1083/jcb.201008007