{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Seok Min Jin"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201008084"],"attach_to":["JCB"],"legend":["HeLa cells co-transfected with YFP-tagged PINK1 R98F mutant and mCherry-Parkin were incubated with DMSO for 1 hr followed by confocal imaging.","YFP tagged PINK1 R98F mutant was transfected into HeLa cells. Cells were stained with Mitotracker Red prior to treatment with CCCP (10 μM) for 3 hrs and analyzed by confocal microscopy.","HeLa cells were transfected with YFP-tagged WT PINK1 for 18 hrs. Cells were then treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS. Cells were immunostained using antibodies against Tom20 (white) and GFP (red) and analyzed by confocal microscopy.","HeLa cells stably expressing YFP-Parkin were treated with DMSO for 3 hrs followed by staining with TMRE and confocal imaging.","HeLa cells transfected with mito-YFP were treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS. Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.","HeLa cells were transfected with YFP-tagged PINK1 R98F mutant for 18 hrs. Cells were then treated with DMSO for 3 hrs, followed by incubation in PBS. Cells were immunostained using antibodies against  Tom20 (white) and GFP (red) and analyzed by confocal microscopy.","HeLa cells transfected with mito-YFP were treated with DMSO for 3 hrs, followed by incubation in PBS containing 0.005% digitonin. Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.","YFP tagged WT PINK1 was transfected into HeLa cells. Cells were stained with Mitotracker Red prior to treatment with DMSO for 3 hrs and analyzed by confocal microscopy.","YFP tagged WT PINK1 was transfected into HeLa cells. Cells were stained with Mitotracker Red prior to treatment with CCCP (10 μM) for 3 hrs and analyzed by confocal microscopy.","YFP tagged PINK1 R98F mutant was transfected into HeLa cells. Cells were stained with Mitotracker Red prior to treatment with DMSO for 3 hrs and analyzed by confocal microscopy.","HeLa cells stably expressing YFP-Parkin were treated with MG132 (10 μM) for 3 hrs followed by staining with TMRE and confocal imaging.","HeLa cells were transfected with YFP-tagged  PINK1 R98F mutant for 18 hrs. Cells were then treated with DMSO for 3 hrs, followed by incubation in PBS containing 0.25% Triton X-100 (TX-100). Cells were immunostained using antibodies against  Tom20 (white) and GFP (red) and analyzed by confocal microscopy.","HeLa cells transfected with mito-YFP were treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS containing 0.25% Trixon X-100 (TX-100). Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.","HeLa cells were transfected with YFP-tagged WT PINK1 for 18 hrs. Cells were then treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS containing 0.25% Triton X-100 (TX-100). Cells were immunostained using antibodies against  Tom20 (white) and GFP (red) and analyzed by confocal microscopy.","HeLa cells co-transfected with YFP-tagged PINK1 R98F mutant and mCherry-Parkin were incubated with CCCP (10 μM) for 1 hr followed by confocal imaging.","PINK1 KO MEFs were transfected with mCherry-Parkin and WT PINK1-YFP. Following treatment with CCCP (10 µM) for 3h, Parkin translocation was examined using Confocal microscopy.","HeLa cells transfected with mito-YFP were treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS containing 0.005% digitonin. Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.","PINK1 KO MEFs were transfected with mCherry-Parkin and ∆[91-117]-PINK1-YFP. Following treatment with DMSO for 3h, Parkin translocation was examined using Confocal microscopy.","HeLa cells stably expressing YFP-Parkin were transfected with control (siCtrl) for 192 hrs. Following transfection, cells were treated with CCCP (10 μM) for 1 hr, stained with TMRE and analyzed by live-cell imaging.","PINK1 KO MEFs were transfected with mCherry-Parkin and WT PINK1-YFP. Following treatment with DMSO for 3h, Parkin translocation was examined using Confocal microscopy.","PINK1 KO MEFs were transfected with mCherry-Parkin and either ∆[91-117]-PINK1-YFP. Following treatment with CCCP (10 µM) for 3h, Parkin translocation was examined using Confocal microscopy.","HeLa cells stably expressing YFP-Parkin were transfected with control (siCtrl) for 192 hrs. Following transfection, cells were treated with DMSO for 1 hr, stained with TMRE and analyzed by live-cell imaging.","HeLa cells were transfected with YFP-tagged WT PINK1 for 18 hrs. Cells were then treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS containing 0.005% digitonin. Cells were immunostained using antibodies against  Tom20 (white) and GFP (red) and analyzed by confocal microscopy.","HeLa cells stably expressing YFP-Parkin were transfected with PARL siRNA (siPARL) for 192 hrs. Following transfection, cells were treated with DMSO for 1 hr, stained with TMRE and analyzed by live-cell imaging.","HeLa cells transfected with mito-YFP were treated with DMSO for 3 hrs, followed by incubation in PBS. Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.","HeLa cells transfected with mito-YFP were treated with DMSO for 3 hrs, followed by incubation in PBS containing 0.25% Trixon X-100 (TX-100). Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.","HeLa cells were transfected with YFP-tagged PINK1 R98F mutant for 18 hrs. Cells were then treated with DMSO for 3 hrs, followed by incubation in PBS containing 0.005% digitonin. Cells were immunostained using antibodies against  Tom20 (white) and GFP (red) and analyzed by confocal microscopy.","HeLa cells stably expressing YFP-Parkin were treated with CCCP (10 μM) for 3 hrs followed by staining with TMRE and confocal imaging."],"repository":["bioimages"],"figure_sub":["Figure 3 - D","Image 26853 (Figure 4 - B)","Image 26810 (Supplementary Figure 1 - C)","Supplementary Figure 1 - C","Image 26793 (Figure 2 - D)","Image 26804 (Figure 4 - C)","Image 26796 (Figure 3 - D)","Image 26845 (Figure 4 - B)","Image 26789 (Figure 1 - F)","Image 26794 (Figure 3 - D)","Figure 4 - B","Image 26808 (Figure 4 - E)","Image 26788 (Figure 1 - F)","Figure 4 - E","Image 26803 (Figure 4 - C)","Figure 4 - C","Image 26795 (Figure 3 - D)","Image 26844 (Figure 4 - B)","Image 26813 (Supplementary Figure 1 - C)","Image 26791 (Figure 2 - D)","Image 26802 (Figure 4 - C)","Image 26807 (Figure 4 - C)","Image 26812 (Supplementary Figure 1 - C)","Figure 1 - F","Image 26843 (Figure 4 - B)","Image 26811 (Supplementary Figure 1 - C)","Figure 4","Image 26854 (Figure 4 - B)","Image 26806 (Figure 4 - C)","Image 26792 (Figure 2 - D)","Image 26790 (Figure 1 - F)","Image 26797 (Figure 3 - D)","Image 26809 (Figure 4 - E)","Image 26805 (Figure 4 - C)","Figure 1","Figure 2 - D","Figure 3","Image 26846 (Figure 4 - B)","Figure 2","Supplementary Figure 1"],"pubmed_authors":["Derek P. Narendra","Lesley A. Kane","Richard J. Youle","Michael Lazarou","Seok Min Jin","Chunxin Wang"],"additional_accession":[]},"is_claimable":false,"name":"Mitochondrial membrane potential regulates PINK1 import and proteolytic destabilization by PARL","description":null,"dates":{"release":"2010-11-29T11:21:07Z","modification":"2018-11-29T11:21:07Z","creation":"2018-11-29T11:21:07Z"},"accession":"S-JCBD-201008084","cross_references":{"doi":["10.1083/jcb.201008084"]}}