bioimagesUnknownSeok Min JinThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201008084JCB

HeLa cells co-transfected with YFP-tagged PINK1 R98F mutant and mCherry-Parkin were incubated with DMSO for 1 hr followed by confocal imaging.

YFP tagged PINK1 R98F mutant was transfected into HeLa cells. Cells were stained with Mitotracker Red prior to treatment with CCCP (10 μM) for 3 hrs and analyzed by confocal microscopy.

HeLa cells were transfected with YFP-tagged WT PINK1 for 18 hrs. Cells were then treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS. Cells were immunostained using antibodies against Tom20 (white) and GFP (red) and analyzed by confocal microscopy.

HeLa cells stably expressing YFP-Parkin were treated with DMSO for 3 hrs followed by staining with TMRE and confocal imaging.

HeLa cells transfected with mito-YFP were treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS. Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.

HeLa cells were transfected with YFP-tagged PINK1 R98F mutant for 18 hrs. Cells were then treated with DMSO for 3 hrs, followed by incubation in PBS. Cells were immunostained using antibodies against Tom20 (white) and GFP (red) and analyzed by confocal microscopy.

HeLa cells transfected with mito-YFP were treated with DMSO for 3 hrs, followed by incubation in PBS containing 0.005% digitonin. Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.

YFP tagged WT PINK1 was transfected into HeLa cells. Cells were stained with Mitotracker Red prior to treatment with DMSO for 3 hrs and analyzed by confocal microscopy.

YFP tagged WT PINK1 was transfected into HeLa cells. Cells were stained with Mitotracker Red prior to treatment with CCCP (10 μM) for 3 hrs and analyzed by confocal microscopy.

YFP tagged PINK1 R98F mutant was transfected into HeLa cells. Cells were stained with Mitotracker Red prior to treatment with DMSO for 3 hrs and analyzed by confocal microscopy.

HeLa cells stably expressing YFP-Parkin were treated with MG132 (10 μM) for 3 hrs followed by staining with TMRE and confocal imaging.

HeLa cells were transfected with YFP-tagged PINK1 R98F mutant for 18 hrs. Cells were then treated with DMSO for 3 hrs, followed by incubation in PBS containing 0.25% Triton X-100 (TX-100). Cells were immunostained using antibodies against Tom20 (white) and GFP (red) and analyzed by confocal microscopy.

HeLa cells transfected with mito-YFP were treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS containing 0.25% Trixon X-100 (TX-100). Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.

HeLa cells were transfected with YFP-tagged WT PINK1 for 18 hrs. Cells were then treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS containing 0.25% Triton X-100 (TX-100). Cells were immunostained using antibodies against Tom20 (white) and GFP (red) and analyzed by confocal microscopy.

HeLa cells co-transfected with YFP-tagged PINK1 R98F mutant and mCherry-Parkin were incubated with CCCP (10 μM) for 1 hr followed by confocal imaging.

PINK1 KO MEFs were transfected with mCherry-Parkin and WT PINK1-YFP. Following treatment with CCCP (10 µM) for 3h, Parkin translocation was examined using Confocal microscopy.

HeLa cells transfected with mito-YFP were treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS containing 0.005% digitonin. Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.

PINK1 KO MEFs were transfected with mCherry-Parkin and ∆[91-117]-PINK1-YFP. Following treatment with DMSO for 3h, Parkin translocation was examined using Confocal microscopy.

HeLa cells stably expressing YFP-Parkin were transfected with control (siCtrl) for 192 hrs. Following transfection, cells were treated with CCCP (10 μM) for 1 hr, stained with TMRE and analyzed by live-cell imaging.

PINK1 KO MEFs were transfected with mCherry-Parkin and WT PINK1-YFP. Following treatment with DMSO for 3h, Parkin translocation was examined using Confocal microscopy.

PINK1 KO MEFs were transfected with mCherry-Parkin and either ∆[91-117]-PINK1-YFP. Following treatment with CCCP (10 µM) for 3h, Parkin translocation was examined using Confocal microscopy.

HeLa cells stably expressing YFP-Parkin were transfected with control (siCtrl) for 192 hrs. Following transfection, cells were treated with DMSO for 1 hr, stained with TMRE and analyzed by live-cell imaging.

HeLa cells were transfected with YFP-tagged WT PINK1 for 18 hrs. Cells were then treated with CCCP (10 μM) for 3 hrs, followed by incubation in PBS containing 0.005% digitonin. Cells were immunostained using antibodies against Tom20 (white) and GFP (red) and analyzed by confocal microscopy.

HeLa cells stably expressing YFP-Parkin were transfected with PARL siRNA (siPARL) for 192 hrs. Following transfection, cells were treated with DMSO for 1 hr, stained with TMRE and analyzed by live-cell imaging.

HeLa cells transfected with mito-YFP were treated with DMSO for 3 hrs, followed by incubation in PBS. Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.

HeLa cells transfected with mito-YFP were treated with DMSO for 3 hrs, followed by incubation in PBS containing 0.25% Trixon X-100 (TX-100). Cells were immunostained using antibodies against Tom20 (white) and Cyt. c (red) and analyzed by confocal microscopy.

HeLa cells were transfected with YFP-tagged PINK1 R98F mutant for 18 hrs. Cells were then treated with DMSO for 3 hrs, followed by incubation in PBS containing 0.005% digitonin. Cells were immunostained using antibodies against Tom20 (white) and GFP (red) and analyzed by confocal microscopy.

HeLa cells stably expressing YFP-Parkin were treated with CCCP (10 μM) for 3 hrs followed by staining with TMRE and confocal imaging.

bioimagesFigure 3 - DImage 26853 (Figure 4 - B)Image 26810 (Supplementary Figure 1 - C)Supplementary Figure 1 - CImage 26793 (Figure 2 - D)Image 26804 (Figure 4 - C)Image 26796 (Figure 3 - D)Image 26845 (Figure 4 - B)Image 26789 (Figure 1 - F)Image 26794 (Figure 3 - D)Figure 4 - BImage 26808 (Figure 4 - E)Image 26788 (Figure 1 - F)Figure 4 - EImage 26803 (Figure 4 - C)Figure 4 - CImage 26795 (Figure 3 - D)Image 26844 (Figure 4 - B)Image 26813 (Supplementary Figure 1 - C)Image 26791 (Figure 2 - D)Image 26802 (Figure 4 - C)Image 26807 (Figure 4 - C)Image 26812 (Supplementary Figure 1 - C)Figure 1 - FImage 26843 (Figure 4 - B)Image 26811 (Supplementary Figure 1 - C)Figure 4Image 26854 (Figure 4 - B)Image 26806 (Figure 4 - C)Image 26792 (Figure 2 - D)Image 26790 (Figure 1 - F)Image 26797 (Figure 3 - D)Image 26809 (Figure 4 - E)Image 26805 (Figure 4 - C)Figure 1Figure 2 - DFigure 3Image 26846 (Figure 4 - B)Figure 2Supplementary Figure 1Derek P. NarendraLesley A. KaneRichard J. YouleMichael LazarouSeok Min JinChunxin WangfalseMitochondrial membrane potential regulates PINK1 import and proteolytic destabilization by PARL2010-11-29T11:21:07Z2018-11-29T11:21:07Z2018-11-29T11:21:07ZS-JCBD-20100808410.1083/jcb.201008084