{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Xin Chen"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201008090"],"attach_to":["JCB"],"legend":["DIC images for figure 5C","Wild type HShh-HA or the processing-defective mutant HShh-C198A-HA were stably expressed in 3T3 cells. Cells were immunostained with rat HA- and rabbit HShh-N- antibodies, followed by goat anti-rat Alexa488 (green) and goat anti-rabbit Alexa594 (red) secondary antibodies. The cells were incubated for 3 hrs with or without the proteasome inhibitor epoxomicin (1 uM). The two left-most panels are control 3T3 cells, treated or not with epoxomicin. These images show the subcellular distribution of HShh-N (red channel).","DIC images.","These images show the subcellular distribution of HShh-C (green channel).","The HShhN ligand was detected with a rabbit antibody, followed by goat anti-rabbit conjugated to Alexa-594","HShhC and the HShh precursor were detected with a rat anti-HA antibody, followed by goat anti-rat conjugated to Alexa-594"],"repository":["bioimages"],"figure_sub":["Figure 5","Image 29511 (Figure 5 - C)","Image 31001 (Figure 5 - C)","Image 31000 (Figure 5 - C)","Image 31002 (Figure 5 - C)","Image 29514 (Figure 5 - C)","Figure 5 - C","Image 29512 (Figure 5 - C)"],"pubmed_authors":["Chih-Hsiang Huang","Tom A. Rapoport","Hanna Tukachinsky","Cindy Jao","Britta Mueller","Sol Schulman","Hsiang-Yun Tang","Xin Chen","Yue-Ru Chu","Adrian Salic"],"additional_accession":[]},"is_claimable":false,"name":"Processing and turnover of the Hedgehog protein in the endoplasmic reticulum","description":null,"dates":{"release":"2011-02-28T11:21:08Z","modification":"2018-11-29T11:21:08Z","creation":"2018-11-29T11:21:08Z"},"accession":"S-JCBD-201008090","cross_references":{"doi":["10.1083/jcb.201008090"]}}