bioimagesUnknownXin ChenThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201008090JCB

DIC images for figure 5C

Wild type HShh-HA or the processing-defective mutant HShh-C198A-HA were stably expressed in 3T3 cells. Cells were immunostained with rat HA- and rabbit HShh-N- antibodies, followed by goat anti-rat Alexa488 (green) and goat anti-rabbit Alexa594 (red) secondary antibodies. The cells were incubated for 3 hrs with or without the proteasome inhibitor epoxomicin (1 uM). The two left-most panels are control 3T3 cells, treated or not with epoxomicin. These images show the subcellular distribution of HShh-N (red channel).

DIC images.

These images show the subcellular distribution of HShh-C (green channel).

The HShhN ligand was detected with a rabbit antibody, followed by goat anti-rabbit conjugated to Alexa-594

HShhC and the HShh precursor were detected with a rat anti-HA antibody, followed by goat anti-rat conjugated to Alexa-594

bioimagesFigure 5Image 29511 (Figure 5 - C)Image 31001 (Figure 5 - C)Image 31000 (Figure 5 - C)Image 31002 (Figure 5 - C)Image 29514 (Figure 5 - C)Figure 5 - CImage 29512 (Figure 5 - C)Chih-Hsiang HuangTom A. RapoportHanna TukachinskyCindy JaoBritta MuellerSol SchulmanHsiang-Yun TangXin ChenYue-Ru ChuAdrian SalicfalseProcessing and turnover of the Hedgehog protein in the endoplasmic reticulum2011-02-28T11:21:08Z2018-11-29T11:21:08Z2018-11-29T11:21:08ZS-JCBD-20100809010.1083/jcb.201008090