{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Yukihiro Yabuta"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201009043"],"attach_to":["JCB"],"legend":["TDRD5-EGFP was costained with MILI in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, MILI.","Electron micrograph of CB in a step 2-3 round spermatid of Tdrd5-wildtype testis.","TDRD5-EGFP was costained with TDRD7 in pachytene spermatocytes. Many TDRD5-EGFP signals, including one in nucleus, localize close to TDRD7 singnal, but not co-stained. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD7.","Histological section of adult epididymus of Tdrd5-wildtype. Hematoxylin and eosin staining.","TDRD5-EGFP was costained with TDRD1 in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD1.","TDRD5-EGFP was costained with TDRD7 in diplotene spermatocytes. TDRD7-positive signals become fewer in number and larger in size in late pachytene-diplotene stage. Most of TDRD7 signals were not costained with TDRD5-EGFP. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD7.","Histological section of adult testis of wildtype control.","Electron micrograph of IMCs in a pachytene spermatocyte of Tdrd5-wildtype testis.","Electron micrograph of CB in a step 5 round spermatid of Tdrd5-knockout testis.","TDRD5-EGFP was costained with TDRD6 in diplotene spermatocytes. TDRD6-positive signals become fewer in number and larger in size in late pachytene-diplotene stage. Most of TDRD6 signals in this stage colocalized with TDRD5-EGFP. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD6.","TDRD5-EGFP costained with TDRD1 in prospermatogonia at E17.5. Only part of the image (up-left area) was shown in figure 2 D a to match the scale to others. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD1.","Z-stack images of confocal sections of stage VIII seminiferous tubule. TDRD5-EGFP and TDRD1 signals are observed at the periphery of nucleus (inter-mitochondrial cements) and within nuclea (T/PNBs) of pachytene spermatocytes at stage VII-VIII seminiferous tubule.  Ch. 1, Hoechst; Ch. 2, TDRD5-EGFP; Ch. 3, TDRD1.","TDRD5-EGFP was costained with TDRD9 in pachytene spermatocytes. TDRD9 signals were very weak at this stage. All TDRD5-EGFP signals were costained with TDRD9.  CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD9.","Electron micrograph of CB in a step 7 round spermatid of Tdrd5-knockout testis. Cytoplasmic bridge is observed at up-right area and CB of a next spermatid are also detected.","TDRD5-EGFP was costained with DCP1A, a marker for piP body, in prospermatogonia at E17.5. Most of the signals were not costained with DCP1A. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, DCP1A.","TDRD5-EGFP was costained with TDRD6 in pachytene spermatocytes. Most of TDRD6 signals, including one in nucleus are contacting to TDRD5-EGFP signals but not costained. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD6.","TDRD5-EGFP in an adult testis section costained with germ cell marker, MVH and DAPI. TDRD5-EGFP expressed in testicular germ cells but not in somatic cells. TDRD5-EGFP signals in the CB of round spermatids are only detectable at early stage (I-III) seminiferous tubule with weak intensity. Therefore, most of the tubules show only MVH signal in round spermatids. CH1, DAPI; CH2,TDRD5-EGFP; CH3, MVH.","Electron micrograph of CB in a step 7 round spermatid of Tdrd5-wildtype testis.","Histological section of adult epididymus of Tdrd5-knockout. Hematoxylin and eosin staining.","Electron micrograph of IMCs in a pachytene spermatocyte of Tdrd5-knockout testis.","Electron micrograph of CB in a step 2-3 round spermatid of Tdrd5-knockout testis.","TDRD5-EGFP was co-stained with MVH in prospermatogonia at E17.5. Granular signal of MVH colocalized with TDRD5-EGFP. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, MVH.","Histological section of adult testis of Tdrd5-knockout testis. Hematoxylin and eosin staining.","TDRD5-EGFP was costained with DCP1A in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, DCP1A.","TDRD5-EGFP was costained with MIWI in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, MIWI.","TDRD5-EGFP was costained with MVH in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, MVH."],"repository":["bioimages"],"figure_sub":["Image 31592 (Figure 3 - G top-right)","Figure 2 - E h","Figure 2 - E i","Figure 2 - E j","Image 31577 (Figure 2 - D a)","Figure 3 - G bottom-left","Image 31582 (Figure 2 - E c)","Image 31598 (Figure 6 - C b)","Figure 2 - E a","Image 31602 (Figure 6 - C f)","Figure 2 - E b","Figure 2 - E c","Figure 2 - E d","Image 31585 (Figure 2 - E f)","Figure 2 - E e","Figure 2 - E f","Figure 2 - E g","Image 31593 (Figure 3 - G bottom-left)","Image 31596 (Figure 6 - B b)","Image 31594 (Figure 3 - G bottom-right)","Figure 3 - G bottom-right","Figure 6 - C f","Image 31589 (Figure 2 - E j)","Image 31599 (Figure 6 - C c)","Figure 3 - G top-right","Image 31575 (SupFigure 2 - B)","Image 31587 (Figure 2 - E h)","Image 31595 (Figure 6 - B a)","Image 31584 (Figure 2 - E e)","Figure 6 - C a","Figure 6 - C c","Figure 6 - C b","Figure 6 - C e","SupFigure 2 - B","Figure 2 - B","Figure 6 - C d","Image 31591 (Figure 3 - G top-left)","Image 31581 (Figure 2 - E b)","Image 31597 (Figure 6 - C a)","Image 31579 (Figure 2 - D b)","Image 31600 (Figure 6 - C d)","Figure 2 - D a","Figure 2 - D b","Figure 2 - D c","SupFigure 2","Image 31586 (Figure 2 - E g)","Image 31583 (Figure 2 - E d)","Image 31601 (Figure 6 - C e)","Image 31590 (Figure 2 - E i)","Image 31576 (Figure 2 - B)","Figure 6","Figure 3 - G top-left","Image 31580 (Figure 2 - E a)","Figure 6 - B b","Figure 6 - B a","Figure 3","Figure 2","Image 31578 (Figure 2 - D c)"],"pubmed_authors":["Mitinori Saitou","Kazuki Kurimoto","Yukihiro Yabuta","Hiroshi Ohta","Shinichiro Chuma","Takaya Abe"],"additional_accession":[]},"is_claimable":false,"name":"TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice","description":null,"dates":{"release":"2011-03-07T11:21:15Z","modification":"2018-11-29T11:21:15Z","creation":"2018-11-29T11:21:15Z"},"accession":"S-JCBD-201009043","cross_references":{"doi":["10.1083/jcb.201009043"]}}