bioimagesUnknownYukihiro YabutaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201009043JCB

TDRD5-EGFP was costained with MILI in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, MILI.

Electron micrograph of CB in a step 2-3 round spermatid of Tdrd5-wildtype testis.

TDRD5-EGFP was costained with TDRD7 in pachytene spermatocytes. Many TDRD5-EGFP signals, including one in nucleus, localize close to TDRD7 singnal, but not co-stained. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD7.

Histological section of adult epididymus of Tdrd5-wildtype. Hematoxylin and eosin staining.

TDRD5-EGFP was costained with TDRD1 in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD1.

TDRD5-EGFP was costained with TDRD7 in diplotene spermatocytes. TDRD7-positive signals become fewer in number and larger in size in late pachytene-diplotene stage. Most of TDRD7 signals were not costained with TDRD5-EGFP. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD7.

Histological section of adult testis of wildtype control.

Electron micrograph of IMCs in a pachytene spermatocyte of Tdrd5-wildtype testis.

Electron micrograph of CB in a step 5 round spermatid of Tdrd5-knockout testis.

TDRD5-EGFP was costained with TDRD6 in diplotene spermatocytes. TDRD6-positive signals become fewer in number and larger in size in late pachytene-diplotene stage. Most of TDRD6 signals in this stage colocalized with TDRD5-EGFP. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD6.

TDRD5-EGFP costained with TDRD1 in prospermatogonia at E17.5. Only part of the image (up-left area) was shown in figure 2 D a to match the scale to others. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD1.

Z-stack images of confocal sections of stage VIII seminiferous tubule. TDRD5-EGFP and TDRD1 signals are observed at the periphery of nucleus (inter-mitochondrial cements) and within nuclea (T/PNBs) of pachytene spermatocytes at stage VII-VIII seminiferous tubule. Ch. 1, Hoechst; Ch. 2, TDRD5-EGFP; Ch. 3, TDRD1.

TDRD5-EGFP was costained with TDRD9 in pachytene spermatocytes. TDRD9 signals were very weak at this stage. All TDRD5-EGFP signals were costained with TDRD9. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD9.

Electron micrograph of CB in a step 7 round spermatid of Tdrd5-knockout testis. Cytoplasmic bridge is observed at up-right area and CB of a next spermatid are also detected.

TDRD5-EGFP was costained with DCP1A, a marker for piP body, in prospermatogonia at E17.5. Most of the signals were not costained with DCP1A. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, DCP1A.

TDRD5-EGFP was costained with TDRD6 in pachytene spermatocytes. Most of TDRD6 signals, including one in nucleus are contacting to TDRD5-EGFP signals but not costained. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, TDRD6.

TDRD5-EGFP in an adult testis section costained with germ cell marker, MVH and DAPI. TDRD5-EGFP expressed in testicular germ cells but not in somatic cells. TDRD5-EGFP signals in the CB of round spermatids are only detectable at early stage (I-III) seminiferous tubule with weak intensity. Therefore, most of the tubules show only MVH signal in round spermatids. CH1, DAPI; CH2,TDRD5-EGFP; CH3, MVH.

Electron micrograph of CB in a step 7 round spermatid of Tdrd5-wildtype testis.

Histological section of adult epididymus of Tdrd5-knockout. Hematoxylin and eosin staining.

Electron micrograph of IMCs in a pachytene spermatocyte of Tdrd5-knockout testis.

Electron micrograph of CB in a step 2-3 round spermatid of Tdrd5-knockout testis.

TDRD5-EGFP was co-stained with MVH in prospermatogonia at E17.5. Granular signal of MVH colocalized with TDRD5-EGFP. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, MVH.

Histological section of adult testis of Tdrd5-knockout testis. Hematoxylin and eosin staining.

TDRD5-EGFP was costained with DCP1A in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, DCP1A.

TDRD5-EGFP was costained with MIWI in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, MIWI.

TDRD5-EGFP was costained with MVH in pachytene spermatocytes. CH1, Hoechst; CH2,TDRD5-EGFP; CH3, MVH.

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