{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Sandra Habbig"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201009069"],"attach_to":["JCB"],"legend":["MCF-10A cells were transfected with FLAG-tagged TAZ and V5-tagged NPHP4. Staining was performed with anti-FLAG pAB (anti-rabbit-IgG-Cy3 - magenta in the merged picture) and anti-V5 mAB (anti-mouse-IgG-Cy2 - green).<br />","siRNA directed against TAZ was transfected into MCF-7 cells. After labeling with BrdU cells were stained anti-BrdU (mAB; anti-mouse-IgG-Cy3 - magenta in the merged image) and counterstained with DAPI (green).","MCF-10A cells were transfected with FLAG-tagged YAP and V5-tagged NPHP4. Staining was performed with anti-FLAG pAB (anti-rabbit-IgG-Cy3 - magenta in the merged picture) and anti-V5 mAB (anti-mouse-IgG-Cy2 - green).","siRNA directed against NPHP4 was transfected into MCF-7 cells. After labeling with BrdU cells were stained anti-BrdU (mAB; anti-mouse-IgG-Cy3 - magenta in the merged image) and counterstained with DAPI (green).","siRNA directed against TAZ and NPHP4 was transfected into MCF-7 cells. After labeling with BrdU cells were stained anti-BrdU (mAB; anti-mouse-IgG-Cy3 - magenta in the merged image) and counterstained with DAPI (green).","Scrambled siRNA was transfected into MCF-7 cells. After labeling with BrdU cells were stained anti-BrdU (mAB; anti-mouse-IgG-Cy3 - magenta in the merged image) and counterstained with DAPI (green)."],"repository":["bioimages"],"figure_sub":["Figure 4 - A","Figure 4","Image 24660 (Figure 4 - A)","Image 24659 (Figure 4 - A)","Image 24661 (Figure 4 - A)","Image 24658 (Figure 2 - C)","Image 24657 (Figure 2 - C)","Image 24662 (Figure 4 - A)","Figure 2 - C","Figure 2"],"pubmed_authors":["Shuhua Chen","Bernhard Schermer","Roman U. Müller","Thomas Benzing","Josef G. Sägmüller","Max C. Liebau","Sandra Habbig","Malte P. Bartram","Nikolaos Andriopoulos","H. Christian Reinhardt","Ricarda Schwarz","Volker Burst","Martin Hoehne"],"additional_accession":[]},"is_claimable":false,"name":"NPHP4, a cilia-associated protein, negatively regulates the Hippo pathway","description":null,"dates":{"release":"2011-05-09T11:21:18Z","modification":"2018-11-29T11:21:18Z","creation":"2018-11-29T11:21:18Z"},"accession":"S-JCBD-201009069","cross_references":{"doi":["10.1083/jcb.201009069"]}}