bioimagesUnknownYayoi IzuThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201010010JCB

In Col12a1+/+ bone, osteoblasts have numerous processes that connect osteoblast to osteoblast. Transmission electron microscopy of the periosteum from cross sections of the femur diaphysis in P14 mice.

Col12a1+/+ osteocytes have processes that extend toward the cortical bone surface interacting with other osteocytes or osteoblasts. Transmission electron microscopy of cross sections of the femur diaphysis in P14 mice.

Col12a1+/+ osteoblasts attached to the surface of cortical bone are elongated and polarized relative to the bone matrix. Transmission electron microscopy of the periosteum from cross sections of the femur diaphysis in P14 mice.

Col12a1-/- osteoblasts have large intracellular spaces with fewer processes than in wild type bone. Transmission electron microscopy of the periosteum from cross sections of the femur diaphysis in P14 mice.

Col12a1-/- osteocytes have fewer and less developed processes than in wild type bone. Transmission electron microscopy of cross sections of the femur diaphysis in P14 mice.

Wild type osteoblasts in an epithelioid arrangement are well organized into a layer along the surface of cortical bone, and F-actin fibers are enriched in regions of the osteoblasts, which face the periostem and cortical bone. DAPI (blue) was used as a nuclear marker; anti-GM130 antibody (red) was used as a Golgi marker; and phalloidin (green) was used to label F-actin.

Wild type osteoblasts exhibit a polarized distribution of the Golgi apparatus and the nucleus. Most Golgi reactivity is localized toward the bone matrix, while the nuclei were positioned to the opposite side. DAPI (blue) was used as a nuclear marker; anti-GM130 antibody (red) was used as a Golgi marker; and phalloidin (green) was used to label F-actin.

Col12a1-/- osteoblasts associated with the surface of cortical bone are disorganized and less polarized than in wild type controls. Transmission electron microscopy of the periosteum from cross sections of the femur diaphysis in P14 mice.

Col12a1-/- osteoblasts are not well organized at the interface of the periosteum and cortical bone, and F-actin fibers appear to form aggregates distributed randomly within osteoblasts. DAPI (blue) was used as a nuclear marker; anti-GM130 antibody (red) was used as a Golgi marker; and phalloidin (green) was used to label F-actin.

Col12a1-/- osteoblasts fail to show a polarized distribution of Golgi and nucleus. It is notable that the F-actin fibers extend to cortical bone matrix establishing a network throughout the bone matrix in wild type bone. This network is not as extensive in Col12a1-/- bone. DAPI (blue) was used as a nuclear marker; anti-GM130 antibody (red) was used as a Golgi marker; and phalloidin (green) was used to label F-actin.

bioimagesImage 33442 (Figure 7 - A)Image 33444 (Figure 7 - B)Image 33365 (Figure 6 - C)Image 33363 (Figure 6 - A)Figure 7 - AFigure 7 - BFigure 7 - DFigure 7Image 33443 (Figure 7 - B)Figure 6Image 33441 (Figure 7 - A)Image 33446 (Figure 7 - D)Image 33364 (Figure 6 - B)Figure 6 - CFigure 6 - DFigure 6 - AImage 33440 (Figure 6 - D)Image 33445 (Figure 7 - D)Figure 6 - BYayoi IzuGuido VeitManuel KochDaniela ZwolanekByeong ChaKarl J. JepsenDavid E. BirkMei SunValerie WilliamsfalseType XII collagen regulates osteoblast polarity and communication during bone formation2011-06-13T11:21:20Z2018-11-29T11:21:20Z2018-11-29T11:21:20ZS-JCBD-20101001010.1083/jcb.201010010