{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Dan Xu"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201010100"],"attach_to":["JCB"],"legend":["Cell morphology of Anti-C-infected young MRC-5 was analyzed at ×100 magnification by phase contrast microscopy.","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in 184hTERT cells.","Hematoxylin and eosin (HE) staining in primary tumor was imaged using a 10× objective with light microscopy on day 46 after inoculation in miR-22-treated mice showing obvious pathological changes in primary tumor.<br />","Selected organs were imaged using IVIS imaging system on day 46 after inoculation in miR-22-treated mice, showing obvious inhibitory effect of miR-22 on tumor metastasis.<br />","Representative photos for SAHF formation in MRC-5 cells at day 6 after transfection of miR-22. Images were taken using a 40× objective with fluorescent microscopy. Enlarged images of the boxed area from the upper panel are shown in this picture.<br /><br />","Representative photos for SAHF formation in MRC-5 cells at day 6 after transfection of miR-22. Images were taken using a 40× objective with fluorescent microscopy.","Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection. GFP-labeled cells indicated infected cells (merged image with phase contrast).","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection of Lenti-C in MDA-D3 cells.","Cell morphology of Anti-22-infected young MRC-5 was analyzed at ×100 magnification by phase contrast microscopy.","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in 184hTERT cells, showing obvious SA-β-gal positive staining.","Mice (5-week-old, CLEA Japan) were inoculated with MDA-MB-231-luc-D3H2LN cells into the fat pad on day 0, followed by injection of control miRNA every other day from day 13 to 31 post-inoculation. Tumor growth was imaged using IVIS imaging system on day 46 after inoculation.","Enlarged images of the boxed area from the upper panel in Fig.7A are shown, showing obvious stress fiber formation in miR-22-transfected cells.","Cell morphology of Anti-22-infected young MRC-5 was analyzed at ×16 magnification by phase contrast microscopy.","Describe: SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection of Lenti-C in SiHa cells.<br />","Cell morphology was analyzed by phase contrast microscopy at day 6 after infection with pre-miR-22 (Lenti-Pre22).","Cell morphology was analyzed by phase contrast microscopy at day 6 after infection of Lenti-C in MDA-D3 cells.","Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in 184hTERT cells.","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in MCF7 cells, showing obvious SA-β-gal positive staining.","Cell morphology was analyzed by phase contrast microscopy at day 6 after infection of Lenti-Pre22 in SiHa cells, showing flattened and enlarged senescent morphology.<br />","Describe: SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection of Lenti-Pre22 in SiHa cells, showing obvious SA-β-gal positive staining.<br />","Cell morphology was analyzed by phase contrast microscopy at day 6 after infection with empty vector (Lenti-C).","Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in SiHa cells.","Presenescent MRC-5 cells infected with Anti-22 were subjected to SA-β-gal assay at day 6 after infection.","Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in 184hTERT cells, showing flattened and enlarged senescent morphology.","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection of Lenti-Pre22 in MDA-D3 cells, showing obvious SA-β-gal positive staining.","Cell morphology was analyzed using a 10× objective by phase contrast microscopy at day 6 after infection of pre-miR-22.","Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of control Anti-C lenti vector. GFP-labeled cells indicated infected cells.","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MDA-D3 cells.","Cell morphology, area, and actin stress fiber formation (stained with phalloidin) were examined by confocal microscopy at day 3 after transfection of miR-22 in SiHa cells, showing enlarged morphology and stress fiber formation.<br />","Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MDA-D3 cells.","Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MCF7 cells.<br />","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in SiHa cells","Cell morphology, area, and actin stress fiber formation (stained with phalloidin) were examined by confocal microscopy at day 3 after transfection of cont miRNA in SiHa cells.<br />","Cell morphology was analyzed using a 10× objective by phase contrast microscopy at day 6 after infection of control Anti-C lenti vector.","Cell morphology was analyzed using a 10× objective by phase contrast microscopy at day 6 after infection.","Enlarged images of the boxed area from the upper panel in Fig.7A (conr miR) are shown.<br />","Histochemical detection of SA-β-gal activity in vivo in primary tumor was imaged using a 40× objective with light microscopy on day 46 after inoculation in cont miR-treated mice. <br />","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection with pre-miR-22 (Lenti-Pre22).","Cell morphology was analyzed using a 10× objective by phase contrast microscopy at day 6 after infection of Anti-miR-22 virus.","Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of Anti-miR-22 virus. GFP-labeled cells indicated infected cells.","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in MDA-D3 cells, showing obvious SA-β-gal positive staining.<br />","Mice (5-week-old, CLEA Japan) were inoculated with MDA-MB-231-luc-D3H2LN cells into the fat pad on day 0, followed by injection of miR-22 every other day from day 13 to 31 post-inoculation. Tumor growth was imaged using IVIS imaging system on day 46 after inoculation, showing obvious inhibitory effect of miR-22 on tumor growth.","Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in SiHa cells, showing flattened and enlarged senescent morphology.","Cell morphology was analyzed by phase contrast microscopy at day 6 after infection of Lenti-C in SiHa cells.","Presenescent MRC-5 cells infected with Anti-C were subjected to SA-β-gal assay at day 6 after infection.","Histochemical detection of SA-β-gal activity in vivo in primary tumor was imaged using a 40× objective with light microscopy on day 46 after inoculation in miR-22-treated mice, showing obvious SA-β-gal positive staining in primary tumor. <br />","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection with empty vector (Lenti-C).","Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of pre-miR-22. GFP-labeled cells indicated infected cells.","Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MDA-D3 cells.<br />","Presenescent MRC-5 cells infected with Anti-22 were subjected to SA-β-gal assay at day 14 after infection.","Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of control Anti--C lenti vector. Phase contrast image was merged with GFP.","Selected organs were imaged using IVIS imaging system on day 46 after inoculation in cont miR-treated mice.<br />","Histochemical detection of SA-β-gal activity in vivo in primary tumor was imaged using a 10× objective with light microscopy on day 46 after inoculation in cont miR-treated mice. <br />","Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of Anti-miR-22 virus. Phase contrast image was merged with GFP.","Representative photos for SAHF formation in MRC-5 cells at day 6 after transfection. Images were taken using a 40× objective with fluorescent microscopy. <br />","Cell morphology of Anti-C-infected young MRC-5 was analyzed at ×16 magnification by phase contrast microscopy.","Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in MDA-D3 cells, showing flattened and enlarged senescent morphology.","Representative photos for SAHF formation in MRC-5 cells at day 6 after transfection. Images were taken using a 40× objective with fluorescent microscopy. Enlarged images of the boxed area from the upper panel are shown in this picture.<br />","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MCF7 cells.","Hematoxylin and eosin (HE) staining in primary tumor was imaged using a 10× objective with light microscopy on day 46 after inoculation in cont miR-treated mice.<br />","Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of pre-miR-22. Phase contrast image was merged with GFP channel.","Cell morphology was analyzed by phase contrast microscopy at day 6 after infection of Lenti-Pre22 in MDA-D3 cells, showing flattened and enlarged senescent morphology.","Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection. GFP-labeled cells indicated infected cells.","Presenescent MRC-5 cells infected with Anti-C were subjected to SA-β-gal assay at day 14 after infection.","Histochemical detection of SA-β-gal activity in vivo in primary tumor was imaged using a 40× objective with light microscopy on day 46 after inoculation in miR-22-treated mice, showing obvious SA-β-gal positive staining in primary tumor.","Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in MCF7 cells, showing flattened and enlarged senescent morphology.","SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in SiHa cells, showing obvious SA-β-gal positive staining."],"repository":["bioimages"],"figure_sub":["Image 31815 (Figure 3 - C)","Figure 8 - E","Image 31857 (Figure 1 - F)","Image 31790 (Figure 2 - A)","Image 31831 (Figure 4 - H)","Figure 8 - D","Image 31863 (Figure 4 - C)","Figure 8 - A","Image 31823 (Figure 3 - E)","Figure 8 - B","Image 31862 (Figure 8 - D)","Figure 7 - A","Image 31840 (Figure 8 - B)","Image 31791 (Figure 2 - C)","Image 31848 (Figure 3 - C)","Image 31799 (Figure 2 - C)","Image 31833 (Figure 4 - H)","Image 31817 (Figure 3 - C)","Image 31855 (Figure 1 - F)","Image 31794 (Figure 2 - C)","Image 31807 (Figure 2 - E)","Image 31805 (Figure 2 - D)","Image 31826 (Figure 3 - E)","Figure 1 - F","Image 31797 (Figure 2 - C)","Image 31846 (Figure 8 - E)","Image 31834 (Figure 4 - H)","Image 31802 (Figure 2 - C)","Image 31818 (Figure 3 - C)","Image 31828 (Figure 4 - C)","Image 31789 (Figure 2 - A)","Image 31812 (Figure 3 - C)","Image 31847 (Figure 8 - E)","Image 31796 (Figure 2 - C)","Image 31804 (Figure 2 - D)","Figure 2 - E","Image 31819 (Figure 3 - C)","Image 31810 (Figure 2 - E)","Image 31841 (Figure 8 - B)","Image 31788 (Figure 2 - A)","Figure 2 - C","Figure 2 - D","Image 31803 (Figure 2 - D)","Figure 2 - A","Image 31801 (Figure 2 - C)","Image 31811 (Figure 3 - C)","Figure 3 - E","Image 31795 (Figure 2 - C)","Image 31849 (Figure 3 - C)","Image 31787 (Figure 2 - A)","Image 31814 (Figure 3 - C)","Image 31800 (Figure 2 - C)","Figure 3 - C","Image 31856 (Figure 1 - F)","Image 31798 (Figure 2 - C)","Image 31861 (Figure 8 - D)","Figure 4 - H","Image 31827 (Figure 4 - C)","Image 31853 (Figure 7 - A)","Figure 4 - C","Image 31852 (Figure 7 - A)","Image 31843 (Figure 8 - D)","Image 31808 (Figure 2 - E)","Image 31821 (Figure 3 - C)","Image 31850 (Figure 3 - C)","Image 31854 (Figure 1 - F)","Image 31793 (Figure 2 - C)","Image 31835 (Figure 7 - A)","Image 31806 (Figure 2 - D)","Image 31825 (Figure 3 - E)","Image 31838 (Figure 8 - A)","Image 31842 (Figure 8 - D)","Image 31809 (Figure 2 - E)","Figure 4","Figure 7","Image 31816 (Figure 3 - C)","Image 31851 (Figure 7 - A)","Image 31832 (Figure 4 - H)","Image 31820 (Figure 3 - C)","Figure 8","Image 31830 (Figure 4 - C)","Image 31824 (Figure 3 - E)","Image 31839 (Figure 8 - A)","Image 31792 (Figure 2 - C)","Figure 1","Figure 3","Figure 2"],"pubmed_authors":["Hidetoshi Tahara","Saori Fukunaga","Fumitaka Takeshita","Takahiro Ochiya","Yasusei Kudo","Akira Shimamoto","Ryou-u Takahashi","Junko Matsunaga","Yumiko Hino","Dan Xu","Aya Tamaki","Takashi Takata"],"additional_accession":[]},"is_claimable":false,"name":"miR-22 represses cancer progression by inducing cellular senescence","description":null,"dates":{"release":"2011-04-18T11:21:25Z","modification":"2018-11-29T11:21:25Z","creation":"2018-11-29T11:21:25Z"},"accession":"S-JCBD-201010100","cross_references":{"doi":["10.1083/jcb.201010100"]}}