bioimagesUnknownDan XuThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201010100JCB

Cell morphology of Anti-C-infected young MRC-5 was analyzed at ×100 magnification by phase contrast microscopy.

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in 184hTERT cells.

Hematoxylin and eosin (HE) staining in primary tumor was imaged using a 10× objective with light microscopy on day 46 after inoculation in miR-22-treated mice showing obvious pathological changes in primary tumor.<br />

Selected organs were imaged using IVIS imaging system on day 46 after inoculation in miR-22-treated mice, showing obvious inhibitory effect of miR-22 on tumor metastasis.<br />

Representative photos for SAHF formation in MRC-5 cells at day 6 after transfection of miR-22. Images were taken using a 40× objective with fluorescent microscopy. Enlarged images of the boxed area from the upper panel are shown in this picture.<br /><br />

Representative photos for SAHF formation in MRC-5 cells at day 6 after transfection of miR-22. Images were taken using a 40× objective with fluorescent microscopy.

Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection. GFP-labeled cells indicated infected cells (merged image with phase contrast).

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection of Lenti-C in MDA-D3 cells.

Cell morphology of Anti-22-infected young MRC-5 was analyzed at ×100 magnification by phase contrast microscopy.

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in 184hTERT cells, showing obvious SA-β-gal positive staining.

Mice (5-week-old, CLEA Japan) were inoculated with MDA-MB-231-luc-D3H2LN cells into the fat pad on day 0, followed by injection of control miRNA every other day from day 13 to 31 post-inoculation. Tumor growth was imaged using IVIS imaging system on day 46 after inoculation.

Enlarged images of the boxed area from the upper panel in Fig.7A are shown, showing obvious stress fiber formation in miR-22-transfected cells.

Cell morphology of Anti-22-infected young MRC-5 was analyzed at ×16 magnification by phase contrast microscopy.

Describe: SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection of Lenti-C in SiHa cells.<br />

Cell morphology was analyzed by phase contrast microscopy at day 6 after infection with pre-miR-22 (Lenti-Pre22).

Cell morphology was analyzed by phase contrast microscopy at day 6 after infection of Lenti-C in MDA-D3 cells.

Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in 184hTERT cells.

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in MCF7 cells, showing obvious SA-β-gal positive staining.

Cell morphology was analyzed by phase contrast microscopy at day 6 after infection of Lenti-Pre22 in SiHa cells, showing flattened and enlarged senescent morphology.<br />

Describe: SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection of Lenti-Pre22 in SiHa cells, showing obvious SA-β-gal positive staining.<br />

Cell morphology was analyzed by phase contrast microscopy at day 6 after infection with empty vector (Lenti-C).

Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in SiHa cells.

Presenescent MRC-5 cells infected with Anti-22 were subjected to SA-β-gal assay at day 6 after infection.

Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in 184hTERT cells, showing flattened and enlarged senescent morphology.

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection of Lenti-Pre22 in MDA-D3 cells, showing obvious SA-β-gal positive staining.

Cell morphology was analyzed using a 10× objective by phase contrast microscopy at day 6 after infection of pre-miR-22.

Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of control Anti-C lenti vector. GFP-labeled cells indicated infected cells.

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MDA-D3 cells.

Cell morphology, area, and actin stress fiber formation (stained with phalloidin) were examined by confocal microscopy at day 3 after transfection of miR-22 in SiHa cells, showing enlarged morphology and stress fiber formation.<br />

Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MDA-D3 cells.

Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MCF7 cells.<br />

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in SiHa cells

Cell morphology, area, and actin stress fiber formation (stained with phalloidin) were examined by confocal microscopy at day 3 after transfection of cont miRNA in SiHa cells.<br />

Cell morphology was analyzed using a 10× objective by phase contrast microscopy at day 6 after infection of control Anti-C lenti vector.

Cell morphology was analyzed using a 10× objective by phase contrast microscopy at day 6 after infection.

Enlarged images of the boxed area from the upper panel in Fig.7A (conr miR) are shown.<br />

Histochemical detection of SA-β-gal activity in vivo in primary tumor was imaged using a 40× objective with light microscopy on day 46 after inoculation in cont miR-treated mice. <br />

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection with pre-miR-22 (Lenti-Pre22).

Cell morphology was analyzed using a 10× objective by phase contrast microscopy at day 6 after infection of Anti-miR-22 virus.

Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of Anti-miR-22 virus. GFP-labeled cells indicated infected cells.

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in MDA-D3 cells, showing obvious SA-β-gal positive staining.<br />

Mice (5-week-old, CLEA Japan) were inoculated with MDA-MB-231-luc-D3H2LN cells into the fat pad on day 0, followed by injection of miR-22 every other day from day 13 to 31 post-inoculation. Tumor growth was imaged using IVIS imaging system on day 46 after inoculation, showing obvious inhibitory effect of miR-22 on tumor growth.

Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in SiHa cells, showing flattened and enlarged senescent morphology.

Cell morphology was analyzed by phase contrast microscopy at day 6 after infection of Lenti-C in SiHa cells.

Presenescent MRC-5 cells infected with Anti-C were subjected to SA-β-gal assay at day 6 after infection.

Histochemical detection of SA-β-gal activity in vivo in primary tumor was imaged using a 40× objective with light microscopy on day 46 after inoculation in miR-22-treated mice, showing obvious SA-β-gal positive staining in primary tumor. <br />

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after infection with empty vector (Lenti-C).

Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of pre-miR-22. GFP-labeled cells indicated infected cells.

Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MDA-D3 cells.<br />

Presenescent MRC-5 cells infected with Anti-22 were subjected to SA-β-gal assay at day 14 after infection.

Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of control Anti--C lenti vector. Phase contrast image was merged with GFP.

Selected organs were imaged using IVIS imaging system on day 46 after inoculation in cont miR-treated mice.<br />

Histochemical detection of SA-β-gal activity in vivo in primary tumor was imaged using a 10× objective with light microscopy on day 46 after inoculation in cont miR-treated mice. <br />

Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of Anti-miR-22 virus. Phase contrast image was merged with GFP.

Representative photos for SAHF formation in MRC-5 cells at day 6 after transfection. Images were taken using a 40× objective with fluorescent microscopy. <br />

Cell morphology of Anti-C-infected young MRC-5 was analyzed at ×16 magnification by phase contrast microscopy.

Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in MDA-D3 cells, showing flattened and enlarged senescent morphology.

Representative photos for SAHF formation in MRC-5 cells at day 6 after transfection. Images were taken using a 40× objective with fluorescent microscopy. Enlarged images of the boxed area from the upper panel are shown in this picture.<br />

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of cont miRNA in MCF7 cells.

Hematoxylin and eosin (HE) staining in primary tumor was imaged using a 10× objective with light microscopy on day 46 after inoculation in cont miR-treated mice.<br />

Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection of pre-miR-22. Phase contrast image was merged with GFP channel.

Cell morphology was analyzed by phase contrast microscopy at day 6 after infection of Lenti-Pre22 in MDA-D3 cells, showing flattened and enlarged senescent morphology.

Cell morphology was analyzed using a 10× objective with fluorescent microscopy at day 6 after infection. GFP-labeled cells indicated infected cells.

Presenescent MRC-5 cells infected with Anti-C were subjected to SA-β-gal assay at day 14 after infection.

Histochemical detection of SA-β-gal activity in vivo in primary tumor was imaged using a 40× objective with light microscopy on day 46 after inoculation in miR-22-treated mice, showing obvious SA-β-gal positive staining in primary tumor.

Cell morphology was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in MCF7 cells, showing flattened and enlarged senescent morphology.

SA-β-gal activity was analyzed by phase contrast microscopy at day 6 after transfection of miR-22 in SiHa cells, showing obvious SA-β-gal positive staining.

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