{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Lidia Hurtado"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201011014"],"attach_to":["JCB"],"legend":["Reassembly of the GA after NZ washout. RPE1 cells co-expressing GT-m-cherry and GFP-AK1B were treated with NZ, and labeling for GMAP210 (green) and gamma-tubulin (red) is shown. Time after NZ washout: 180 min.","RPE-1 cells were cotransfected with VSV-GFP and flag-AK1. After transfection, cells were incubated at 40ºC and were then transferred to 32ºC and fixed at 100 min. Cells were labelled for GFP (green) and AKAP450(red).","RPE-1 cells were transfected with VSV-GFP(control). After transfection, cells were incubated at 40ºC and were then transferred to 32ºC and fixed at 60 min. Cells were labelled for GFP (green) and AKAP450(red).","High magnification image of a flag-AK1-transfected cell triple labelled for α-tubulin (green), flag (red) and GMAP210 (blue) to visualize MT network.","A flag-AK1-transfected cell incubated on ice for 40 min to depolymerize MTs was triple stained for α-tubulin (green), flag (red) and GMAP210 (blue).","RPE-1 cells were transfected with VSV-GFP. After transfection, cells were incubated at 40ºC and were then transferred to 32ºC and fixed at 0 min. Cells were labelled for GFP (green) and AKAP450(red).","Subcellular localization of GFP-AK1 (green). A GFP-AK1-expressing cell was labelled for GMAP210 (red).","Low magnification image of a flag-AK1B transfected cells triple labelled for flag (green), AKAP450 (red) and GMAP210 (blue).","Merged image of a flag-AK1-transfected cell stained with α-tubulin (green), flag (red) and GMAP210 (blue) to visualize the MT network.","Control cells were transfected with the flag-AK1B construct and then NZ treated to fragment the GA into stacks. Labellings were for flag (green), GM130 (red) and GMAP210 (blue).","RPE-1 cells were transfected with VSV-GFP(control). After transfection, cells were incubated at 40ºC and were then transferred to 32ºC and fixed at 100 min. Cells were labelled for GFP (green) and AKAP450(red).","RPE-1 cells were cotransfected with VSV-GFP and flag-AK1. After transfection, cells were incubated at 40ºC and were then transferred to 32ºC and fixed at 0 min. Cells were labelled for GFP (green) and AKAP450(red).","GM130-depleted cells were transfected with the flag-AK1B construct and then NZ treated to fragment the GA into stacks. Labelings were for flag (green), GM130 (red) and GMAP210 (blue).","FRAP experiments were performed in RPE-1 GT-GFP expressing cells co-transfected with mcherry-centrin and flag-AK1B. An image of a selected cell is  shown before bleaching (pre-bleach) with CTR in red and the GA in green.","RPE-1 cells were cotransfected with VSV-GFP and flag-AK1B. After transfection, cells were incubated at 40ºC and were then transferred to 32ºC and fixed at 0 min. Cells were labelled for GFP (green) and AKAP450(red).","Merged image of a cell expressing flag-AK1B triple labelled for flag (green), GM130 (red) and Golgin245 (blue).","FRAP experiments were performed in RPE-1 GT-GFP expressing cells co-transfected with mcherry-centrin and flag-AK1B. A video from of a selected cell is shown 0 sec after bleaching with CTR in red and the GA in green.","Representative images of control cells maintained in 0.25% serum for 36h and triple labelled for polyglutamylated tubulin (green), AKAP450 (red) and GMAP210 (blue).","Merged image of a flag-AK1B-expressing cell triple labelled for flag (green), AKAP450 (red) and GMAP210 (blue).","Translocation of Venus-NPY containing vesicules in AK1-expressing cells. Images recorded over 2 min were overlaid to visualize the vesicle tracks.","Reorientation of the CTR and the GA after wounding in AK1-transfected cells. Cells were stained with flag (green), AKAP450 (red)and GMAP210 (blue).","Merged image of a cell expressing flag-AK1 triple labelled for flag (green), GM130 (red) and Golgin245 (blue).","FRAP experiments were performed in RPE-1 GT-GFP expressing cells co-transfected with mcherry-centrin and either flag-AK1. An image of a selected cell is shown before bleaching (pre-bleach) with CTR in red and the GA in green.","Merged image of a flag-AK1B-expresssing cell stained with α-tubulin (green), flag (red) and GMAP210 (blue) to visualize the MT network.","A control cell incubated on ice for 40 min to depolymerize MTs and rewarmed for 2 min was triple stained for α-tubulin (green), flag (red) and GMAP210 (blue).","RPE-1 cells were transfected with VSV-GFP (control). VSV-GFP was detected at the PM by incubating with the ectodomain antibody 8G5 during the last 30 min of a 60 or 100 min period after the shift at a permissive temperature. Cells were then fixed with methanol and triple labelled for 8G5 (green), AKAP450 (red) and GMAP210 (blue).","RPE-1 cells were cotransfected with VSV-GFP and flag-AK1B. After transfection, cells were incubated at 40ºC and were then transferred to 32ºC and fixed at 60 min. Cells were labelled for GFP (green) and AKAP450(red).","RPE-1 cells were cotransfected with VSV-GFP and flag-AK1. VSV-GFP was detected at the PM by incubating with the ectodomain antibody 8G5 during the last 30 min o a 60 or 100 min period after the shift at a permissive temperature. Cells were then fixed and triple labelled for 8G5 (green), AKAP450(red) and GMAP210 (blue).","FRAP experiments were performed in RPE-1 GT-GFP expressing cells co-transfected with mcherry-centrin and flag-AK1B. A video frame of a selected cell is shown 200 sec after bleaching with CTR in red and the GA in green.","A flag-AK1-transfected cell incubated on ice for 40 min to depolymerize MTs and rewarmed for 7 min was triple labelled for α-tubulin (green), flag (red) and GMAP210 (blue).","Control cells were treated with NZ to depolymerise MTs. At 15 min after washout, cells were triple labelled for α-tubulin (green), flag (red) and GMAP210 (blue).","Reassembly of the GA after NZ washout. RPE1 cells co-expressing GT-m-cherry and GFP-AK1 were treated with NZ, and labeling for GMAP210 (green) and gamma-tubulin (red) is shown. Time after NZ washout: 0 min.","Flag-AK1B transfected cells were treated with NZ to depolymerise MTs. Before washout, cells were triple labelled for α-tubulin (green), flag (red) and GMAP210 (blue).","Reorientation of the CTR and the GA after wounding in AK1B-transfected cells. Cells were stained with flag (green), AKAP450 (red) and GMAP210 (blue).","A control cell incubated on ice for 40 min to depolymerize MTs and rewarmed for 7 min was triple stained for α-tubulin (green), flag (red) and GMAP210 (blue).","Representative images flag-AK1 transfected cells maintained in 0.25% serum for 36h and triple labelled for polyglutamylated tubulin (green), AKAP450 (red) and GMAP210 (blue).","FRAP experiments were performed in RPE-1 GT-GFP expressing cells co-transfected with mcherry-centrin and flag-AK1. A video frame of a selected cell is shown 200 sec after bleaching with CTR in red and the GA in green.","Reassembly of the GA after NZ washout. RPE1 cells expressing GT-mcherry were treated with NZ, and labeling for GMAP210 (green) and gamma-tubulin (red) is shown. Time after NZ washout: 180 min.","A flag-AK1-transfected cell incubated on ice for 40 min to depolymerize MTs and rewarmed for 2 min was triple stained for α-tubulin (green), flag (red) and GMAP210 (blue).","Flag-AK1B transfected cells were treated with NZ to depolymerise MTs. At 5 min after washout, cells were triple labelled for α-tubulin (green), flag (red) and GMAP210 (blue).","FRAP experiments were performed in RPE-1 GT-GFP expressing cells transfected with mcherry-centrin. A video frame of a selected cells is shown 200 sec after bleaching with CTR in red and the GA in green.","Representative images flag-AK1B transfected cells maintained in 0.25% serum for 36h and triple labelled for polyglutamylated tubulin (green), AKAP450 (red) and GMAP210 (blue).","Low magnification image of a flag-AK1 transfected cells triple labelled for flag (green), AKAP450 (red) and GMAP210 (blue).","FRAP experiments were performed in RPE-1 GT-GFP expressing cells co-transfected with mcherry-centrin and flag-AK1. A video frame of a selected cell is shown 0 sec after bleaching with CTR in red and the GA in green.","FRAP experiments were performed in RPE-1 GT-GFP expressing cells transfected with mcherry-centrin. An image of a selected cell is shown before bleaching (pre-bleach) with CTR in red and the GA in green.","RPE-1 cells were cotransfected with VSV-GFP and flag-AK1B. After transfection, cells were incubated at 40ºC and were then transferred to 32ºC and fixed at 100 min. Cells were labelled for GFP (green) and AKAP450(red).","RPE-1 cell expressing the flag-AK1 fragment was triple labelled for flag (green), AKAP450 (red) and GMAP210 (blue).","Control cells were treated with NZ to depolymerise MTs. At 5 min after washout, cells were triple labelled for α-tubulin (green), flag (red) and GMAP210 (blue).","Reassembly of the GA after NZ washout. RPE1 cells co-expressing GT-m-cherry and GFP-AK1B were treated with NZ, and labeling for GMAP210 (green) and gamma-tubulin (red) is shown. Time after NZ washout: 0 min.","RPE-1 cells were cotransfected with VSV-GFP and flag-AK1. After transfection, cells were incubated at 40ºC and were then transferred to 32ºC and fixed at 60 min. Cells were labelled for GFP (green) and AKAP450(red).","A control cell incubated on ice for 40 min to depolymerize MTs was triple stained for α-tubulin (green), flag (red) and GMAP210 (blue).","RPE-1 cells were cotransfected with VSV-GFP and flag-AK1B. VSV-GFP was detected at the PM by incubating with the ectodomain antibody 8G5 during the last 30 min o a 60 or 100 min period after the shift at a permissive temperature. Cells were then fixed and triple labelled for 8G5 (green), AKAP450 (red) and GMAP210 (blue).","Reassembly of the GA after NZ washout. RPE1 cells co-expressing GT-m-cherry and GFP-AK1 were treated with NZ, and labeling for GMAP210 (green) and gamma-tubulin (red) is shown. Time after NZ washout: 180 min.","FRAP experiments were performed in RPE-1 GT-GFP expressing cells transfected with mcherry-centrin. A video frame of a selected cell is shown 0 sec after bleaching (pre-bleach) with CTR in red and the GA in gree.","Translocation of Venus-NPY containing vesicules in AK1B-expressing cells. Images recorded over 2 min were overlaid to visualize the vesicle tracks.","Control cells were treated with NZ to depolymerise MTs. Before washout, cells were triple labelled for α-tubulin (green), flag (red) and GMAP210 (blue).","Reassembly of the GA after NZ washout. RPE1 cells expressing GT-mcherry were treated with NZ, and labeling for GMAP210 (green) and gamma-tubulin (red) is shown. Time after NZ washout: 0 min.","Subcellular localization of GFP-AK4 (green). A GFP-AK4 expressing cell was labelled for γ-tubulin (red).","Flag-AK1B transfected cells were treated with NZ to depolymerise MTs. At 15 min after washout, cells were triple labelled for α-tubulin (green), flag (red) and GMAP210 (blue).","Translocation of Venus-NPY containing vesicules in control cells. Images recorded over 2 min were overlaid to visualize the vesicle tracks.","Subcellular localization of GFP-AK1B (green). A GFP-AK1B expressing cell was labelled for GMAP210 (red)."],"repository":["bioimages"],"figure_sub":["Image 32829 (Figure 9 - B)","Image 32762 (Figure 1 - C)","Image 32788 (Figure 4 - A)","Image 32765 (Figure 1 - H)","Figure 8 - A","Image 32809 (Figure 7 - B)","Image 32799 (Figure 6 - A)","Image 32818 (Figure 7 - B)","Figure 7 - A","Figure 7 - B","Image 32770 (Figure 2 - F)","Figure 7 - C","Image 32794 (Figure 5 - A)","Figure 9 - B","Image 32790 (Figure 4 - B)","Image 32811 (Figure 7 - B)","Image 32828 (Figure 5 - A)","Image 32779 (Figure 4 - A)","Image 32808 (Figure 7 - A)","Image 32773 (Figure 3 - C)","Image 32803 (Figure 6 - C)","Image 32767 (Figure 2 - C)","Image 32789 (Figure 4 - B)","Image 32797 (Figure 5 - A)","Figure 1 - H","Image 32821 (Figure 7 - C)","Image 32781 (Figure 4 - A)","Image 32777 (Figure 4 - A)","Image 32768 (Figure 2 - D)","Image 32784 (Figure 4 - B)","Image 32780 (Figure 4 - A)","Image 32820 (Figure 7 - C)","Image 32822 (Figure 8 - A)","Figure 1 - E","Image 32814 (Figure 7 - B)","Figure 1 - C","Figure 1 - B","Image 32783 (Figure 2 - G)","Figure 2 - G","Figure 2 - E","Image 32776 (Figure 4 - A)","Figure 2 - F","Image 32763 (Figure 1 - E)","Image 32806 (Figure 7 - A)","Image 32813 (Figure 7 - B)","Figure 2 - C","Image 32775 (Figure 3 - C)","Figure 2 - D","Figure 2 - A","Image 32769 (Figure 2 - E)","Image 32782 (Figure 2 - G)","Image 32793 (Figure 5 - A)","Image 32807 (Figure 7 - A)","Image 32764 (Figure 1 - H)","Figure 3 - C","Figure 4 - A","Figure 4 - B","Image 32823 (Figure 8 - A)","Image 32785 (Figure 4 - B)","Image 32798 (Figure 6 - A)","Image 32801 (Figure 6 - B)","Image 32815 (Figure 7 - B)","Image 32810 (Figure 7 - B)","Image 32766 (Figure 2 - A)","Image 32827 (Figure 5 - A)","Figure 5 - A","Image 32800 (Figure 6 - B)","Image 32830 (Figure 9 - B)","Image 32831 (Figure 5 - A)","Image 32802 (Figure 6 - C)","Image 32796 (Figure 5 - A)","Image 32816 (Figure 7 - B)","Figure 5","Figure 4","Figure 7","Image 32826 (Figure 5 - A)","Figure 6","Figure 9","Image 32761 (Figure 1 - B)","Figure 8","Figure 6 - C","Image 32791 (Figure 4 - B)","Image 32795 (Figure 5 - A)","Figure 6 - A","Figure 6 - B","Figure 1","Image 32819 (Figure 7 - C)","Figure 3","Image 32817 (Figure 7 - B)","Figure 2","Image 32787 (Figure 4 - B)","Image 32824 (Figure 9 - B)"],"pubmed_authors":["Lidia Hurtado","Maria P. Gavilan","Jesus Cardenas","Michel Bornens","Cristina Caballero","Rosa M. Rios"],"additional_accession":[]},"is_claimable":false,"name":"Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis","description":null,"dates":{"release":"2011-05-23T11:21:30Z","modification":"2018-11-29T11:21:30Z","creation":"2018-11-29T11:21:30Z"},"accession":"S-JCBD-201011014","cross_references":{"doi":["10.1083/jcb.201011014"]}}