{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Iwona M. Pranke"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201011118"],"attach_to":["JCB"],"legend":["Shown is a brightfield image corresponding to localization of alpha-synuclein chimera-GFP (green) with mCherry-Bos1p (red) in a yeast cell after 2.5 hours of induction.","Shown is localization of GFP-Emp47p (green), an ER-Golgi cargo receptor, in a cell also expressing invALPS-GMAP-mCherry. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.","Shown is a transmission EM image of a yeast cell expressing ALPS-Smc1-CC-GFP after overnight induction.","Shown is a brightfield image corresponding to localization of GMAP-N-mCherry with pro-alpha-factor-Citrine in SEY6210 yeast cells after overnight induction.","Shown is a transmission EM image of a yeast cell expressing ALPS-Smc1-CC-GFP after overnight induction. This is an additional example not included in the figure.","Shown is localization of pro-alpha-factor-Citrine (green) in SEY6210 yeast cells also expressing GMAP-N-mCherry (red) after overnight induction.","Shown is localization of GMAP-N-mCherry in yeast cells after overnight induction. An overlay of brightfield and fluorescence is shown.","Shown is localization of alpha-synuclein-GFP in wild-type yeast cells after overnight induction.","Shown is localization of the endocytic tracer FM-64 in cells expressing invALPS-GMAP-mCherry (red). This is an image not included in figure but corresponds to the quantification shown in Figure 8D.","Shown is localization of GMAP-N-mCherry (red) in SEY6210  yeast cells also expressing pro-alpha-factor-Citrine after overnight induction.","Shown is localization of delta-ALPS-GMAP-N-mCherry (GMAP-N with ALPS motif deleted) in a yeast cell after overnight induction.","Shown is localization of alpha-synuclein chimera-GFP (green) with mCherry-Snc1p after 4 hours of induction. This image is not included in figure but corresponds to quantification shown in Figure 7C.","Shown is a brightfield image corresponding to localization of invALPS-GMAP-mCherry with GFP-Sec22p, an ER-Golgi cargo receptor. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.","Shown is localization of GMAP-N-mCherry (red) in yeast cells after overnight induction.","Shown is localization of GFP-Sec22p, an ER-Golgi SNARE protein, in cells also expressing invALPS-GMAP-mCherry. This image was not included in the figure but corresponds to the quantification shown in Figure 8D.","Shown is a brightfield image corresponding to localization of alpha-synuclein-GFP in a wild-type yeast cell after 3 hr induction. This is an additional example not included in the figure.","Shown is a transmission EM image of a yeast cell expressing invALPS-GMAP-mCherry after overnight induction.","Shown is localization of alpha-synuclein-GFP (green) in a wild-type yeast cell after 3 hr induction. This is an additional example not included in the figure.","Shown is a brightfield image corresponding to localization of GMAP-N-GFP in BY4742 wild-type yeast cells after overnight induction. This image is shown in Supplemental Figure S1A, 2u, lower panel.","Shown is a brightfield image corresponding to localization of alpha-synuclein chimera-GFP with mCherry-Snc1p after 4 hours of induction. This image is not included in figure but corresponds to quantification shown in Figure 7C.","Shown is localization of mCherry-Bos1p (red), in a yeast cell also expressing alpha-synuclein chimera-GFP, after 2.5 hours of induction.","Shown is a brightfield image corresponding to localization of GMAP-N-mCherry in yeast cells after overnight induction.","Transmission EM image of a yeast cell expressing GMAP-N-GFP after overnight induction. This is an additional example not included in figure.","Shown is a transmission EM image of a yeast cell expressing the alpha-synuclein chimera-GFP after overnight induction.","Shown is localization of mCherry-Snc1p (red), in a yeast cell also expressing alpha-synuclein chimera-GFP (green), after 2.5 hours of induction. This is an additional example not included in Figure 7B.","Shown is a brightfield image corresponding to localization of invALPS-GMAP-mCherry with the endocytic tracer FM4-64. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.","Shown is localization image of GMAP-N-GFP (green) in BY4742 wild-type yeast cells after 3 hours induction. This is an additional example not included in the figure.","Shown is immunogold labeling of yeast cells expressing GMAP-N-GFP after overnight induction, using antibodies against GFP. The image shown in Supplemental Fig. S1B.","Shown is localization image of GMAP-N-GFP (green) in BY4742 wild-type yeast cells after overnight induction. This image is shown in Supplemental Figure S1A, 2u, lower panel.","Shown is localization of alpha-synuclein-GFP (green) in a wild-type yeast cell after overnight induction. This is an additional example not included in the figure.","Shown is localization of mCherry-Snc1p (red) with alpha-synuclein chimera-GFP (green) after 4 hours of induction. This image is not included in figure but corresponds to quantification shown in Figure 7C.","Shown is a transmission EM image of a yeast cell expressing invALPS-GMAP-mCherry after overnight induction. This is an additional example not included in the figure.","Shown is immunogold labeling of yeast cells expressing GMAP-N-GFP after overnight induction, using antibodies against GFP.","Shown is a transmission EM image of a yeast cell expressing the alpha-synuclein chimera-GFP after overnight induction.<br />","Shown is localization of the T6 mutant alpha-synuclein-GFP in wild-type yeast cells after overnight induction.","Transmission EM image of a yeast cell expressing GMAP-N-GFP after overnight induction.","Shown is a brightfield image corresponding to localization of alpha-synuclein chimera-GFP (green) with mCherry-Snc1p (red) in a yeast cell after 2.5 hours of induction. This is an additional example not included in Figure 7B.","Transmission EM image of a yeast cell expressing GMAP-N-GFP after overnight induction. This is an additional example not included in figure.<br />","Shown is a brightfield image corresponding to localization of invALPS-GMAP-mCherry with GFP-Emp47p, an ER-Golgi cargo receptor. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.","Shown is a brightfield image corresponding to localization of alpha-synuclein-GFP in a wild-type yeast cell after overnight induction. This is an additional example not included in the figure.","Shown is immunogold labeling of yeast cells expressing GMAP-N-GFP after overnight induction, using antibodies against GFP. This is an additional example not included in figure.<br />","Shown is localization of invALPS-GMAP-mCherry (red) in a cell also expressing GFP-Emp47p, an ER-Golgi cargo receptor. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.","Shown is localization image of GMAP-N-GFP (green) in BY4742 wild-type yeast cells after overngiht induction. This is an additional example not included in the figure.","Shown is a transmission EM image of yeast cell expressing alpha-synuclein-GFP after overnight induction.","Shown is the brightfield image corresponding to localization of the T6 mutant alpha-synuclein-GFP in wild-type yeast cells after overnight induction.","Shown is a brightfield image corresponding to localization of alpha-synuclein-GFP in yeast cells after overnight induction.","Shown is localization of invALPS-GMAP-mCherry (red) in a cell also stained for the endocytic tracer FM-64. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.","Shown is fluorescence imaging for ocalization of alpha-synuclein-GFP (green) in yeast cells after overnight induction.","Shown is a transmission EM image of a yeast cell expressing the alpha-synuclein chimera-GFP after overnight induction. This is an additional image not shown in figure.","Shown is a brightfield image corresponding to localization image of GMAP-N-GFP in BY4742 wild-type yeast cells after 3 hours induction. This is an additional example not included in the figure.","Shown is a brightfield image corresponding to localization of alpha-synuclein-GFP in wild-type yeast cells after overnight induction.","Shown is localization of delta-ALPS-GMAP-N-mCherry (GMAP-N with ALPS motif deleted; red) in a yeast cell after overnight induction.","Shown is localization of alpha-synuclein chimera-GFP (green), in a yeast cell also expressing mCherry-Bos1p, after 2.5 hours of induction.","Shown is a transmission EM image of yeast cell expressing alpha-synuclein-GFP after overnight induction. This is an additional example not included in figure.<br /><br />","Shown is localization of invALPS-GMAP-mCherry (red) in a cell also expressing GFP-Sec22p, an ER-Golgi SNARE protein. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.","Shown is localization of alpha-synuclein chimera-GFP (green), in a yeast cell also expressing mCherry-Snc1p, after 2.5 hours of induction. This is an additional example not included in Figure 7B."],"repository":["bioimages"],"figure_sub":["Image 28651 (Figure 6 - F and G)","Image 28667 (Figure 1 - E)","Image 28616 (Figure 7 - A)","Figure 8 - B","Image 28627 (Figure 8 - D-Sec22p)","Image 31553 (SupFigure 3 - C)","Image 31541 (SupFigure 1 - A)","Image 28669 (Figure 5 - C)","Image 31539 (SupFigure 1 - A)","Image 31547 (Figure 6 - A)","Image 31552 (SupFigure 3 - C)","Image 28642 (Figure 7 - B)","Image 28612 (Figure 5 - A)","Image 28723 (SupFigure 4 - A)","Image 28645 (Figure 7 - C)","Image 28672 (Figure 8 - B)","Image 28659 (Figure 1 - B)","Figure 1 - A","Image 28673 (Figure 8 - B)","Figure 1 - E","Image 28648 (Figure 7 - C)","Figure 1 - D","Figure 1 - C","SupFigure 3 - C","Figure 1 - B","Image 28537 (Figure 1 - A)","Image 31544 (Figure 6 - A)","Image 28722 (SupFigure 4 - A)","Image 28652 (Figure 6 - F and G)","Image 28539 (Figure 1 - C)","Figure 8 - D-Emp47p","Image 28646 (Figure 7 - C)","Image 31550 (SupFigure 3 - C)","SupFigure 1","Image 28661 (Figure 1 - B)","SupFigure 3","Image 28657 (Figure 1 - B)","Image 31555 (Figure 6 - A)","SupFigure 4","Image 28633 (Figure 8 - D-FM4-64)","Image 28538 (Figure 1 - A)","Image 28615 (Figure 7 - A)","Figure 5 - A","Image 28644 (Figure 7 - C)","Image 28640 (Figure 7 - B)","Figure 5 - C","Image 28629 (Figure 8 - D-Sec22p)","Figure 5","Figure 7","Figure 6","Figure 8","Image 28676 (Figure 8 - B)","Image 28650 (Figure 6 - F and G)","Image 28663 (Figure 1 - D)","Figure 1","Image 28665 (Figure 1 - E)","Image 28617 (Figure 7 - A)","Image 28631 (Figure 8 - D-FM4-64)","Image 31542 (SupFigure 1 - A)","Image 28624 (Figure 8 - D-Emp47p)","Image 28656 (Figure 6 - F and G)","Figure 7 - A","Figure 8 - D-Sec22p","Image 28660 (Figure 1 - B)","Figure 7 - B","Figure 7 - C","Figure 6 - F and G","Image 28671 (Figure 8 - B)","Image 28625 (Figure 8 - D-Emp47p)","Image 28674 (Figure 8 - B)","SupFigure 4 - A","Image 28611 (Figure 5 - A)","Image 28655 (Figure 6 - F and G)","Image 31545 (Figure 6 - A)","Image 28647 (Figure 7 - C)","Image 28658 (Figure 1 - B)","Image 28536 (Figure 1 - A)","Image 28721 (SupFigure 4 - A)","Image 28675 (Figure 8 - B)","Image 31543 (SupFigure 1 - A)","Image 28668 (Figure 1 - E)","Image 28654 (Figure 6 - F and G)","Image 28632 (Figure 8 - D-FM4-64)","SupFigure 1 - A","Image 28666 (Figure 1 - E)","Image 28653 (Figure 6 - F and G)","Image 31546 (Figure 6 - A)","Image 28641 (Figure 7 - B)","Image 28662 (Figure 1 - D)","Image 28670 (Figure 5 - C)","Image 31554 (Figure 6 - A)","Image 28664 (Figure 1 - E)","Image 28623 (Figure 8 - D-Emp47p)","Figure 6 - A","Image 28628 (Figure 8 - D-Sec22p)","Image 31551 (SupFigure 3 - C)","Image 28540 (Figure 1 - C)","Image 31538 (SupFigure 1 - A)","Image 28643 (Figure 7 - C)","Figure 8 - D-FM4-64"],"pubmed_authors":["Vincent Morello","Iwona M. Pranke","Catherine L. Jackson","Joëlle Bigay","Kimberley Gibson","Bruno Antonny","Jean-Marc Verbavatz"],"additional_accession":[]},"is_claimable":false,"name":"α-Synuclein and ALPS motifs are membrane curvature sensors whose contrasting chemistry mediates selective vesicle binding","description":null,"dates":{"release":"2011-07-11T11:21:38Z","modification":"2018-11-29T11:21:38Z","creation":"2018-11-29T11:21:38Z"},"accession":"S-JCBD-201011118","cross_references":{"doi":["10.1083/jcb.201011118"]}}