bioimagesUnknownIwona M. PrankeThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201011118JCB

Shown is a brightfield image corresponding to localization of alpha-synuclein chimera-GFP (green) with mCherry-Bos1p (red) in a yeast cell after 2.5 hours of induction.

Shown is localization of GFP-Emp47p (green), an ER-Golgi cargo receptor, in a cell also expressing invALPS-GMAP-mCherry. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.

Shown is a transmission EM image of a yeast cell expressing ALPS-Smc1-CC-GFP after overnight induction.

Shown is a brightfield image corresponding to localization of GMAP-N-mCherry with pro-alpha-factor-Citrine in SEY6210 yeast cells after overnight induction.

Shown is a transmission EM image of a yeast cell expressing ALPS-Smc1-CC-GFP after overnight induction. This is an additional example not included in the figure.

Shown is localization of pro-alpha-factor-Citrine (green) in SEY6210 yeast cells also expressing GMAP-N-mCherry (red) after overnight induction.

Shown is localization of GMAP-N-mCherry in yeast cells after overnight induction. An overlay of brightfield and fluorescence is shown.

Shown is localization of alpha-synuclein-GFP in wild-type yeast cells after overnight induction.

Shown is localization of the endocytic tracer FM-64 in cells expressing invALPS-GMAP-mCherry (red). This is an image not included in figure but corresponds to the quantification shown in Figure 8D.

Shown is localization of GMAP-N-mCherry (red) in SEY6210 yeast cells also expressing pro-alpha-factor-Citrine after overnight induction.

Shown is localization of delta-ALPS-GMAP-N-mCherry (GMAP-N with ALPS motif deleted) in a yeast cell after overnight induction.

Shown is localization of alpha-synuclein chimera-GFP (green) with mCherry-Snc1p after 4 hours of induction. This image is not included in figure but corresponds to quantification shown in Figure 7C.

Shown is a brightfield image corresponding to localization of invALPS-GMAP-mCherry with GFP-Sec22p, an ER-Golgi cargo receptor. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.

Shown is localization of GMAP-N-mCherry (red) in yeast cells after overnight induction.

Shown is localization of GFP-Sec22p, an ER-Golgi SNARE protein, in cells also expressing invALPS-GMAP-mCherry. This image was not included in the figure but corresponds to the quantification shown in Figure 8D.

Shown is a brightfield image corresponding to localization of alpha-synuclein-GFP in a wild-type yeast cell after 3 hr induction. This is an additional example not included in the figure.

Shown is a transmission EM image of a yeast cell expressing invALPS-GMAP-mCherry after overnight induction.

Shown is localization of alpha-synuclein-GFP (green) in a wild-type yeast cell after 3 hr induction. This is an additional example not included in the figure.

Shown is a brightfield image corresponding to localization of GMAP-N-GFP in BY4742 wild-type yeast cells after overnight induction. This image is shown in Supplemental Figure S1A, 2u, lower panel.

Shown is a brightfield image corresponding to localization of alpha-synuclein chimera-GFP with mCherry-Snc1p after 4 hours of induction. This image is not included in figure but corresponds to quantification shown in Figure 7C.

Shown is localization of mCherry-Bos1p (red), in a yeast cell also expressing alpha-synuclein chimera-GFP, after 2.5 hours of induction.

Shown is a brightfield image corresponding to localization of GMAP-N-mCherry in yeast cells after overnight induction.

Transmission EM image of a yeast cell expressing GMAP-N-GFP after overnight induction. This is an additional example not included in figure.

Shown is a transmission EM image of a yeast cell expressing the alpha-synuclein chimera-GFP after overnight induction.

Shown is localization of mCherry-Snc1p (red), in a yeast cell also expressing alpha-synuclein chimera-GFP (green), after 2.5 hours of induction. This is an additional example not included in Figure 7B.

Shown is a brightfield image corresponding to localization of invALPS-GMAP-mCherry with the endocytic tracer FM4-64. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.

Shown is localization image of GMAP-N-GFP (green) in BY4742 wild-type yeast cells after 3 hours induction. This is an additional example not included in the figure.

Shown is immunogold labeling of yeast cells expressing GMAP-N-GFP after overnight induction, using antibodies against GFP. The image shown in Supplemental Fig. S1B.

Shown is localization image of GMAP-N-GFP (green) in BY4742 wild-type yeast cells after overnight induction. This image is shown in Supplemental Figure S1A, 2u, lower panel.

Shown is localization of alpha-synuclein-GFP (green) in a wild-type yeast cell after overnight induction. This is an additional example not included in the figure.

Shown is localization of mCherry-Snc1p (red) with alpha-synuclein chimera-GFP (green) after 4 hours of induction. This image is not included in figure but corresponds to quantification shown in Figure 7C.

Shown is a transmission EM image of a yeast cell expressing invALPS-GMAP-mCherry after overnight induction. This is an additional example not included in the figure.

Shown is immunogold labeling of yeast cells expressing GMAP-N-GFP after overnight induction, using antibodies against GFP.

Shown is a transmission EM image of a yeast cell expressing the alpha-synuclein chimera-GFP after overnight induction.<br />

Shown is localization of the T6 mutant alpha-synuclein-GFP in wild-type yeast cells after overnight induction.

Transmission EM image of a yeast cell expressing GMAP-N-GFP after overnight induction.

Shown is a brightfield image corresponding to localization of alpha-synuclein chimera-GFP (green) with mCherry-Snc1p (red) in a yeast cell after 2.5 hours of induction. This is an additional example not included in Figure 7B.

Transmission EM image of a yeast cell expressing GMAP-N-GFP after overnight induction. This is an additional example not included in figure.<br />

Shown is a brightfield image corresponding to localization of invALPS-GMAP-mCherry with GFP-Emp47p, an ER-Golgi cargo receptor. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.

Shown is a brightfield image corresponding to localization of alpha-synuclein-GFP in a wild-type yeast cell after overnight induction. This is an additional example not included in the figure.

Shown is immunogold labeling of yeast cells expressing GMAP-N-GFP after overnight induction, using antibodies against GFP. This is an additional example not included in figure.<br />

Shown is localization of invALPS-GMAP-mCherry (red) in a cell also expressing GFP-Emp47p, an ER-Golgi cargo receptor. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.

Shown is localization image of GMAP-N-GFP (green) in BY4742 wild-type yeast cells after overngiht induction. This is an additional example not included in the figure.

Shown is a transmission EM image of yeast cell expressing alpha-synuclein-GFP after overnight induction.

Shown is the brightfield image corresponding to localization of the T6 mutant alpha-synuclein-GFP in wild-type yeast cells after overnight induction.

Shown is a brightfield image corresponding to localization of alpha-synuclein-GFP in yeast cells after overnight induction.

Shown is localization of invALPS-GMAP-mCherry (red) in a cell also stained for the endocytic tracer FM-64. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.

Shown is fluorescence imaging for ocalization of alpha-synuclein-GFP (green) in yeast cells after overnight induction.

Shown is a transmission EM image of a yeast cell expressing the alpha-synuclein chimera-GFP after overnight induction. This is an additional image not shown in figure.

Shown is a brightfield image corresponding to localization image of GMAP-N-GFP in BY4742 wild-type yeast cells after 3 hours induction. This is an additional example not included in the figure.

Shown is a brightfield image corresponding to localization of alpha-synuclein-GFP in wild-type yeast cells after overnight induction.

Shown is localization of delta-ALPS-GMAP-N-mCherry (GMAP-N with ALPS motif deleted; red) in a yeast cell after overnight induction.

Shown is localization of alpha-synuclein chimera-GFP (green), in a yeast cell also expressing mCherry-Bos1p, after 2.5 hours of induction.

Shown is a transmission EM image of yeast cell expressing alpha-synuclein-GFP after overnight induction. This is an additional example not included in figure.<br /><br />

Shown is localization of invALPS-GMAP-mCherry (red) in a cell also expressing GFP-Sec22p, an ER-Golgi SNARE protein. This is an image not included in figure but corresponds to the quantification shown in Figure 8D.

Shown is localization of alpha-synuclein chimera-GFP (green), in a yeast cell also expressing mCherry-Snc1p, after 2.5 hours of induction. This is an additional example not included in Figure 7B.

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