{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Eliza Żyłkiewicz"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201011142"],"attach_to":["JCB"],"legend":["Image showing formation of unfocused microtubule structures in Xenopus egg extracts in the presence of function blocking xNdel1 antibodies(anti-xNdel1).","Image showing xNdel1 co-localization with dynein IC (DIC) in the center of Ran aster.","Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of additional LIS1 (xNdel1+LIS1).","Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 88-192 (xNdel1+88-192).<br /><br />","Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 (xNdel1+8-192)","Image showing DMSO-induced aster formation in control Xenopus egg extracts in the presence of control rabbit IgG (IgG).","Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E36A/E39A (xNdel1+8-192 E36A/E39A).","Image showing formation of multiple foci in xNdel1-depleted Xenopus egg extracts in the presence of LIS1 (xNdel1+LIS1).","Image showing formation of hollow asters in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E119A/R130A and LIS1 (xNdel1+8-192 E119A/R130A+LIS1).","Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-310 E48A/E52A (xNdel1+8-310 E48A/E52A).","Image showing aster formation in control depleted Xenopus egg extracts in the presence of BSA (IgG+BSA).<br /><br />","Image showing formation of unfocused microtubule structures in Xenopus egg extracts in the presence of a dominant negative p50/dynamitin subunit of dynactin(p50/dynamitin).","Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNde1 7-170 (xNdel1+mNde1 7-170).","Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of control BSA (xNdel1+BSA).","Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 (xNdel1+8-192).","Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 1-174 (xNdel1+1-174).","Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-310 (xNdel1+8-310).","Image showing aster formation in control Xenopus egg extracts in the presence of control rabbit IgG (IgG).","Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E48A/E52A (xNdel1+8-192 E48A/E52A).","Image showing aster formation in control depleted Xenopus egg extracts in the presence of BSA (IgG+BSA).","Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNde1 7-99 (xNdel1+mNde1 7-99).","Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E119A/R130A and LIS1(xNdel1+8-192 E119A/R130A+LIS1).","Image showing formation of microtubule bundles in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E119A/R130A (xNdel1+8-192 E119A/R130A).","Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of control BSA (xNdel1+BSA).<br /><br />","Image showing aster formation in control depleted Xenopus egg extracts in the presence of BSA (IgG+BSA).<br />Note: Images are shown for the same experiment as in Figure 3 e, and the same control IgG depletion is presented.","Image showing formation of unfocused microtubule structures in Xenopus egg extracts in the presence of function blocking DIC antibodies(anti-DIC).","Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-99 (xNdel1+8-99)."],"repository":["bioimages"],"figure_sub":["Image 28999 (Figure 2 - b')","Image 29850 (Figure 1 - d)","Figure 1 - d","Figure 1 - c","Image 29046 (Figure 5 - b)","Image 29043 (Figure 5 - b)","Image 29004 (Figure 3 - e)","Figure 2 - b'","Image 29006 (Figure 3 - e)","Image 28998 (Figure 2 - b')","Image 29008 (Figure 4 - c)","Image 29010 (Figure 4 - c)","Image 29044 (Figure 5 - b)","Image 28995 (Figure 2 - b')","Image 29005 (Figure 3 - e)","Image 31524 (Figure 2 - d')","Image 29007 (Figure 4 - c)","Image 29045 (Figure 5 - b)","Image 29042 (Figure 5 - b)","Image 29003 (Figure 3 - e)","Figure 5 - b","Image 29329 (Figure 1 - c)","Figure 5 - d","Image 29048 (Figure 5 - d)","Image 28997 (Figure 2 - b')","SupFigure 2","Image 29014 (SupFigure 2 - b)","SupFigure 5","Image 29851 (Figure 1 - d)","Image 29009 (Figure 4 - c)","Image 29047 (Figure 5 - b)","Image 31522 (Figure 2 - d')","Image 31525 (Figure 2 - d')","SupFigure 2 - b","Figure 3 - e","Image 29001 (Figure 3 - e)","SupFigure 5 - b","Image 29849 (Figure 1 - d)","Figure 2 - d'","Image 29852 (Figure 1 - d)","Image 29013 (SupFigure 2 - b)","Image 29011 (SupFigure 5 - b)","Figure 5","Figure 4","Image 29002 (Figure 3 - e)","Image 28996 (Figure 2 - b')","Image 29000 (Figure 2 - b')","Figure 1","Image 29012 (SupFigure 5 - b)","Figure 3","Figure 4 - c","Figure 2"],"pubmed_authors":["Won-Chan Choi","Urszula Derewenda","Monika Kijańska","Zygmunt S. Derewenda","P. Todd Stukenberg","Eliza Żyłkiewicz"],"additional_accession":[]},"is_claimable":false,"name":"The N-terminal coiled-coil of Ndel1 is a regulated scaffold that recruits LIS1 to dynein","description":null,"dates":{"release":"2011-01-31T11:21:41Z","modification":"2018-11-29T11:21:41Z","creation":"2018-11-29T11:21:41Z"},"accession":"S-JCBD-201011142","cross_references":{"doi":["10.1083/jcb.201011142"]}}