bioimagesUnknownEliza ŻyłkiewiczThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201011142JCB

Image showing formation of unfocused microtubule structures in Xenopus egg extracts in the presence of function blocking xNdel1 antibodies(anti-xNdel1).

Image showing xNdel1 co-localization with dynein IC (DIC) in the center of Ran aster.

Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of additional LIS1 (xNdel1+LIS1).

Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 88-192 (xNdel1+88-192).<br /><br />

Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 (xNdel1+8-192)

Image showing DMSO-induced aster formation in control Xenopus egg extracts in the presence of control rabbit IgG (IgG).

Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E36A/E39A (xNdel1+8-192 E36A/E39A).

Image showing formation of multiple foci in xNdel1-depleted Xenopus egg extracts in the presence of LIS1 (xNdel1+LIS1).

Image showing formation of hollow asters in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E119A/R130A and LIS1 (xNdel1+8-192 E119A/R130A+LIS1).

Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-310 E48A/E52A (xNdel1+8-310 E48A/E52A).

Image showing aster formation in control depleted Xenopus egg extracts in the presence of BSA (IgG+BSA).<br /><br />

Image showing formation of unfocused microtubule structures in Xenopus egg extracts in the presence of a dominant negative p50/dynamitin subunit of dynactin(p50/dynamitin).

Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNde1 7-170 (xNdel1+mNde1 7-170).

Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of control BSA (xNdel1+BSA).

Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 (xNdel1+8-192).

Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 1-174 (xNdel1+1-174).

Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-310 (xNdel1+8-310).

Image showing aster formation in control Xenopus egg extracts in the presence of control rabbit IgG (IgG).

Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E48A/E52A (xNdel1+8-192 E48A/E52A).

Image showing aster formation in control depleted Xenopus egg extracts in the presence of BSA (IgG+BSA).

Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNde1 7-99 (xNdel1+mNde1 7-99).

Image showing rescue of aster formation in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E119A/R130A and LIS1(xNdel1+8-192 E119A/R130A+LIS1).

Image showing formation of microtubule bundles in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-192 E119A/R130A (xNdel1+8-192 E119A/R130A).

Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of control BSA (xNdel1+BSA).<br /><br />

Image showing aster formation in control depleted Xenopus egg extracts in the presence of BSA (IgG+BSA).<br />Note: Images are shown for the same experiment as in Figure 3 e, and the same control IgG depletion is presented.

Image showing formation of unfocused microtubule structures in Xenopus egg extracts in the presence of function blocking DIC antibodies(anti-DIC).

Image showing formation of unfocused microtubule structures in xNdel1-depleted Xenopus egg extracts in the presence of mNdel1 8-99 (xNdel1+8-99).

bioimagesImage 28999 (Figure 2 - b')Image 29850 (Figure 1 - d)Figure 1 - dFigure 1 - cImage 29046 (Figure 5 - b)Image 29043 (Figure 5 - b)Image 29004 (Figure 3 - e)Figure 2 - b'Image 29006 (Figure 3 - e)Image 28998 (Figure 2 - b')Image 29008 (Figure 4 - c)Image 29010 (Figure 4 - c)Image 29044 (Figure 5 - b)Image 28995 (Figure 2 - b')Image 29005 (Figure 3 - e)Image 31524 (Figure 2 - d')Image 29007 (Figure 4 - c)Image 29045 (Figure 5 - b)Image 29042 (Figure 5 - b)Image 29003 (Figure 3 - e)Figure 5 - bImage 29329 (Figure 1 - c)Figure 5 - dImage 29048 (Figure 5 - d)Image 28997 (Figure 2 - b')SupFigure 2Image 29014 (SupFigure 2 - b)SupFigure 5Image 29851 (Figure 1 - d)Image 29009 (Figure 4 - c)Image 29047 (Figure 5 - b)Image 31522 (Figure 2 - d')Image 31525 (Figure 2 - d')SupFigure 2 - bFigure 3 - eImage 29001 (Figure 3 - e)SupFigure 5 - bImage 29849 (Figure 1 - d)Figure 2 - d'Image 29852 (Figure 1 - d)Image 29013 (SupFigure 2 - b)Image 29011 (SupFigure 5 - b)Figure 5Figure 4Image 29002 (Figure 3 - e)Image 28996 (Figure 2 - b')Image 29000 (Figure 2 - b')Figure 1Image 29012 (SupFigure 5 - b)Figure 3Figure 4 - cFigure 2Won-Chan ChoiUrszula DerewendaMonika KijańskaZygmunt S. DerewendaP. Todd StukenbergEliza ŻyłkiewiczfalseThe N-terminal coiled-coil of Ndel1 is a regulated scaffold that recruits LIS1 to dynein2011-01-31T11:21:41Z2018-11-29T11:21:41Z2018-11-29T11:21:41ZS-JCBD-20101114210.1083/jcb.201011142