{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Anja Mai"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201012126"],"attach_to":["JCB"],"legend":["Recycling assay following control siRNA with 10 nm gold-conjugated, anti-beta1-integrin antibody and detected with electron microscopy. Integrin location after recycling is indicated (n=35-50 cells scored). Scale bars are 0.2 um.","Recycling assay following p120 siRNA with 10 nm gold-conjugated, anti-beta1-integrin antibody and detected with electron microscopy. Integrin location after recycling is indicated (n=35-50 cells scored). Scale bars are 0.2 um.","Rab21-EGFP expressing cells treated with p120RasGAP siRNA were surface labelled with 10 nm gold-conjugated anti-beta1-integrin antibody. Subsequently, cells were allowed to undergo internalisation followed by serum-induced recycling for 30 min respectively. Rab21-EGFP was co-labelled with 5 nm protein A-gold. Shown are electron microscopic images of cells from frozen thin cryosections. Scale bar represents 200 nm.","Rab21-EGFP expressing cells treated with control siRNA were surface labelled with 10 nm gold-conjugated anti-beta1-integrin antibody. Subsequently, cells were allowed to undergo internalisation followed by serum-induced recycling for 30 min respectively. Rab21-EGFP was co-labelled with 5 nm protein A-gold. Shown are electron microscopic images of cells from frozen thin cryosections. Scale bar represents 200 nm."],"repository":["bioimages"],"figure_sub":["Image 33177 (Figure 7 - C)","Figure 7","Image 33176 (Figure 7 - C)","Image 33174 (Figure 1 - D)","Image 33175 (Figure 1 - D)","Figure 7 - C","Figure 1","Figure 1 - D"],"pubmed_authors":["Varpu Marjomäki","Eleanor Coffey","Teijo Pellinen","Anja Mai","Stefan Veltel","Artur Padzik","Johanna Ivaska"],"additional_accession":[]},"is_claimable":false,"name":"Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration","description":null,"dates":{"release":"2011-07-18T11:21:46Z","modification":"2018-11-29T11:21:46Z","creation":"2018-11-29T11:21:46Z"},"accession":"S-JCBD-201012126","cross_references":{"doi":["10.1083/jcb.201012126"]}}