bioimagesUnknownAnja MaiThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201012126JCB

Recycling assay following control siRNA with 10 nm gold-conjugated, anti-beta1-integrin antibody and detected with electron microscopy. Integrin location after recycling is indicated (n=35-50 cells scored). Scale bars are 0.2 um.

Recycling assay following p120 siRNA with 10 nm gold-conjugated, anti-beta1-integrin antibody and detected with electron microscopy. Integrin location after recycling is indicated (n=35-50 cells scored). Scale bars are 0.2 um.

Rab21-EGFP expressing cells treated with p120RasGAP siRNA were surface labelled with 10 nm gold-conjugated anti-beta1-integrin antibody. Subsequently, cells were allowed to undergo internalisation followed by serum-induced recycling for 30 min respectively. Rab21-EGFP was co-labelled with 5 nm protein A-gold. Shown are electron microscopic images of cells from frozen thin cryosections. Scale bar represents 200 nm.

Rab21-EGFP expressing cells treated with control siRNA were surface labelled with 10 nm gold-conjugated anti-beta1-integrin antibody. Subsequently, cells were allowed to undergo internalisation followed by serum-induced recycling for 30 min respectively. Rab21-EGFP was co-labelled with 5 nm protein A-gold. Shown are electron microscopic images of cells from frozen thin cryosections. Scale bar represents 200 nm.

bioimagesImage 33177 (Figure 7 - C)Figure 7Image 33176 (Figure 7 - C)Image 33174 (Figure 1 - D)Image 33175 (Figure 1 - D)Figure 7 - CFigure 1Figure 1 - DVarpu MarjomäkiEleanor CoffeyTeijo PellinenAnja MaiStefan VeltelArtur PadzikJohanna IvaskafalseCompetitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration2011-07-18T11:21:46Z2018-11-29T11:21:46Z2018-11-29T11:21:46ZS-JCBD-20101212610.1083/jcb.201012126