{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["M. Rizwan Siddiqui"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201012129"],"attach_to":["JCB"],"legend":["Z-stack images of Cav-1–/– endothelial monolayers pre-treated with L-NNA and stained for VE-cadherin (green), b-catenin (magenta), F-actin (red) and nuclei (blue). Images were acquired using a Zeiss LSM 510 META confocal microscope equipped with c-Apochromat 63x/1.2 water correction objective and the following filter sets: Alexa-488: BP 505/530; Alexa-532: BP 560/615 and Cy5: LP 650.<br />","Z-stack images of Wt endothelial monolayers stained for VE-cadherin (green), beta-catenin (magenta), F-actin (red) and nuclei (blue). Images were acquired using a Zeiss LSM 510 META confocal microscope equipped with c-Apochromat 63x/1.2 water correction objective and the following filter sets: Alexa-488: BP 505/530; Alexa-532: BP 560/615 and Cy5: LP 650.","Immunofluorescent staining of HPAECs for eNOS (green) and b-cat (red); DAPI (blue); 3 min after stimulation with 50 nM a-thrombin","VE-cadherin distribution in Wt endothelial monolayers: 12-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).<br /><br />","Organization of actin cytoskeleton in Cav-1–/–  endothelial monolayers: 12-bit projected image.","Confocal microscopy images of control and Ga13 siRNA lentivirus-infected confluent HMVEC-L in response to 300M H2O2, 2g/ml LPS, or 20ng/ml TNFa.","Confocol microscopy images of VE-cadherin subcellular location in mScr or mG13BP pre-treated HMVEC-L in the presence or absence of chloroquine for 3 hr. Green: VE-cadherin. Arrows pointed to internalized VE-cadherin.","Beta-catenin distribution in Cav-1–/– endothelial monolayers pretreated with RhoA inhibitor: 12-bit projected image.","Confocal microscopy images of confluent HMVEC-L pre-treated with PBS, 150uM mScr, or 150uM mG13BP in response to PBS, 300uM H2O2, 2ug/ml LPS, or 20ng/ml TNFa.","Immunofluorescent staining of HPAECs for eNOS (green) and b-cat (red); DAPI (blue); 15 min after stimulation with 50 nM a-thrombin","Organization of actin cytoskeleton in Cav-1–/– endothelial monolayers pre-treated with Rho inhibitor C3 transferase: 12-bit projected image.","Organization of actin cytoskeleton in eNOS–/–/Cav-1–/– double KO endothelial monolayers: 12-bit projected image. <br /><br />","VE-cadherin distribution in Cav-1–/– endothelial monolayers: 12-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).","Organization of actin cytoskeleton in Cav-1–/– endothelial monolayers: 16-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).<br />","Z-stack images of Cav-1–/– endothelial monolayers stained for VE-cadherin (green), beta-catenin (magenta), F-actin (red) and nuclei (blue). Images were acquired using a Zeiss LSM 510 META confocal microscope equipped with c-Apochromat 63x/1.2 water correction objective and the following filter sets: Alexa-488: BP 505/530; Alexa-532: BP 560/615 and Cy5: LP 650.<br />","Beta-catenin distribution in Cav-1–/– endothelial monolayers: 12-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).<br />","Immunofluorescent staining of HPAECs for eNOS (green) and b-cat (red); DAPI (blue); untreated cells.","VE-cadherin distribution in Cav-1–/– endothelial monolayers pretreated with L-NNA: 16-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).","Z-stack images of Cav-1–/– endothelial monolayers pre-treated with AP-CSD peptide and stained for beta-catenin (green), F-actin (red) and nuclei (blue). Images were acquired using a Zeiss LSM 510 META confocal microscope.","Organization of actin cytoskeleton in Cav-1–/– endothelial monolayers pretreated with L-NNA: 16-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).<br />","Live cell images of a FRET-based RhoA biosensor in Wt endothelial monolayers. Z-stack images were obtained using a confocal microscope (Zeiss LSM 510 META) equipped with 63X, 1.2 NA water immersion correction objective and an Ar ion laser. For emission ratio imaging, we acquired 12-bit CFP (blue; l=458 nm; BP500/20), FRET (red; l=458 nm; LP530) and YFP (yellow; l=514 nm; LP530) images. <br />","Beta-catenin distribution in eNOS–/–/Cav-1–/– double KO endothelial monolayers: 12-bit projected image. <br />","Organization of actin cytoskeleton in Wt endothelial monolayers: 12-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).","Beta-catenin distribution in Wt endothelial monolayers: 12-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).","Beta-catenin distribution in Cav-1–/– endothelial monolayers: 12-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).","Live cell images of a FRET-based RhoA biosensor in Cav-1-/- endothelial monolayers. Z-stack images were obtained using a confocal microscope (Zeiss LSM 510 META) equipped with 63X, 1.2 NA water immersion correction objective and an Ar ion lasers. For emission ratio imaging, we acquired 12-bit CFP (blue; l=458 nm; BP500/20), FRET (red; l=458 nm; LP530) and YFP (yellow; l=514 nm; LP530) images. <br />","Confocal microscopy images of VE-cadherin expression in mouse PMECs isolated from WT and Gna13flox/flox mice infected with Cre recombinase adenovirus.","Z-stack images of eNOS–/–/Cav-1–/– double KO endothelial monolayers stained for beta-catenin (green), F-actin (red) and nuclei (blue). Images were acquired using a Zeiss LSM 510 META confocal microscope<br />","Organization of actin cytoskeleton in Cav-1–/– endothelial monolayers pretreated with AP-CSD peptide: 12-bit projected image.","Beta-catenin distribution in Cav-1–/– endothelial monolayers pretreated with L-NNA: 16-bit projected image. The maximum pixel intensity was collected from each image of Z-stack and projected to the single image using MetaMorph software (Molecular Devices).","Z-stack images of Cav-1–/– endothelial monolayers stained for VE-cadherin (green), beta-catenin (magenta), F-actin (red) and nuclei (blue). Images were acquired using a Zeiss LSM 510 META confocal microscope equipped with c-Apochromat 63x/1.2 water correction objective.","Z-stack images of Cav-1–/– endothelial monolayers pretreated with Rho inhibitor C3 transferase and stained for beta-catenin (green), F-actin (red) and nuclei (blue). Images were acquired using a Zeiss LSM 510 META confocal microscope equipped with c-Apochromat 63x/1.2 water correction objective.<br />","Beta-catenin distribution in Cav-1–/– endothelial monolayers pretreated with AP-CSD peptide: 12-bit projected image. <br />","FRET/CFP ratio images in Cav-1-/- endothelial monolayers. Pixel intensities of ratio images were scaled from 0 to 5 and color-coded.","FRET/CFP ratio images (RhoA activity) in Wt endothelial monolayers. Pixel intensities of ratio images were scaled from 0 to 5 and color-coded."],"repository":["bioimages"],"figure_sub":["Image 29720 (Fig 4 - a)","Image 29607 (Fig 1 - c)","Image 290392 (Fig 5 - Fig5E)","Image 29646 (Fig 1 - c)","Image 290388 (Fig 1 - Fig1C)","Image 32860 (Fig 5 - a)","Image 29605 (Fig 1 - c)","Fig 1 - c","Image 29644 (Fig 1 - c)","Image 29647 (Fig 1 - c)","Image 29648 (Fig 1 - c)","Image 29600 (Fig 1 - c)","Image 29604 (Fig 1 - c)","Image 290385 (Fig 1 - Fig1C)","Image 32843 (Fig 4 - a)","Image 290382 (Fig 1 - Fig1C)","Image 290396 (Fig 5 - Fig5E)","Image 290366 (Fig 1 - Fig1I)","Image 290370 (Fig 1 - Fig1I)","Image 290369 (Fig 1 - Fig1I)","Image 29640 (Fig 1 - c)","Image 32834 (Fig 1 - c)","Image 29641 (Fig 1 - c)","Fig 3 - c","Image 29719 (Fig 4 - a)","Image 29706 (Fig 4 - a)","Image 29707 (Fig 4 - a)","Image 290395 (Fig 5 - Fig5E)","Image 290373 (Fig 5 - Fig5C)","Image 29703 (Fig 4 - a)","Fig 4 - a","Image 29715 (Fig 4 - a)","Image 290376 (Fig 5 - Fig5C)","Image 290386 (Fig 1 - Fig1C)","Image 32854 (Fig 5 - a)","Image 29639 (Fig 1 - c)","Image 32852 (Fig 5 - a)","Image 290380 (Fig 1 - Fig1C)","Image 290383 (Fig 1 - Fig1C)","Image 290394 (Fig 5 - Fig5E)","Image 290397 (Fig 5 - Fig5E)","Image 290389 (Fig 1 - Fig1C)","Image 290367 (Fig 1 - Fig1I)","Fig 5 - a","Image 290372 (Fig 5 - Fig5C)","Image 32835 (Fig 3 - c)","Image 290375 (Fig 5 - Fig5C)","Image 290379 (Fig 1 - Fig1C)","Image 32836 (Fig 3 - c)","Image 290390 (Fig 5 - Fig5E)","Image 290393 (Fig 5 - Fig5E)","Image 290378 (Fig 1 - Fig1C)","Image 29659 (Fig 3 - c)","Image 290374 (Fig 5 - Fig5C)","Fig 5 - Fig5E","Fig 5 - Fig5C","Image 32889 (Fig 4 - a)","Image 290377 (Fig 5 - Fig5C)","Image 32838 (Fig 4 - a)","Image 290368 (Fig 1 - Fig1I)","Image 32837 (Fig 4 - a)","Fig 5","Image 290391 (Fig 5 - Fig5E)","Fig 4","Image 290381 (Fig 1 - Fig1C)","Image 290384 (Fig 1 - Fig1C)","Image 290387 (Fig 1 - Fig1C)","Fig 1 - Fig1C","Image 29655 (Fig 3 - c)","Image 29693 (Fig 4 - a)","Fig 3","Image 29671 (Fig 4 - a)","Fig 1","Fig 1 - Fig1I","Image 290371 (Fig 1 - Fig1I)"],"pubmed_authors":["M. Rizwan Siddiqui","You-Yang Zhao","Stephen M. Vogel","Xiaopei Gao","Marcelo G. Bonini","Johnson Rajasingh","Asrar B. Malik","Viktor Brovkovych","Yulia A. Komarova"],"additional_accession":[]},"is_claimable":false,"name":"Caveolin-1–eNOS signaling promotes p190RhoGAP-A nitration and endothelial permeability","description":null,"dates":{"release":"2011-05-30T11:21:47Z","modification":"2018-11-29T11:21:47Z","creation":"2018-11-29T11:21:47Z"},"accession":"S-JCBD-201012129","cross_references":{"doi":["10.1083/jcb.201012129"]}}