{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Meredith E.K. Calvert"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201101055"],"attach_to":["JCB"],"legend":["A Z-stack of optical sections from a representative hypha during actomyosin ring constriction, showing the localization of Lifeact-GFP (green), FM4-64 (red), and calcofluor (blue) to label actin, cell membrane and cell wall, respectively. See also Supplemental Video 3.","Time-lapse imaging shows the decrease in ring diameter over time within a representative hypha expressing Lifeact-GFP (green) and membranes labeled with FM4-64 (red). <br />","A Z-stack of optical sections showing adjacent actomyosin rings immediately following the photobleaching of Myo-2-GFP (green). The ring on the right has been bleached. See also Supplemental Video 4.","Time-lapse imaging shows that the actin binding probe Lifeact-GFP accumulated along the hyphal cortex, assembled into a contractile ring, and persisted at the ring throughout septation.  See also Supplemental video 2.","Time-lapse imaging shows the decrease in ring diameter over time within a representative hypha expressing Myo2-GFP (green) and membranes labeled with FM4-64 (red). See also Supplemental Video 1.","Time-lapse imaging shows the decrease in ring diameter over time within a representative hypha expressing Lifeact-GFP (green) and membranes labeled with FM4-64 (red).","Single image showing Lifeact-GFP (green) and membranes labeled with FM4-64 (red) following inhibition of actin polymerization with 10 um Latrunculin A.","Delocalization of Myo-2-GFP (green) from the ring and stalled membrane closure (FM4-64, red) following treatement with 1mM blebbistatin","Time-lapse of Z-stacks of optical sections showing adjacent actomyosin rings during fluorescence recovery after photobleaching (FRAP) of Myo2-GFP (green). The ring on the right has been bleached. See also Supplemental Video 4.","Time-lapse imaging shows Lifeact-GFP (green) and membranes labeled with FM4-64 (red) following treatment with DMSO.","Time-lapse imaging shows the decrease in ring diameter over time within a representative hypha expressing Myo2-GFP (green) and membranes labeled with FM4-64 (red).","A Z-stack of optical sections showing adjacent actomyosin rings prior to the photobleaching of Myo-2-GFP (green). See also Supplemental Video 4.<br />","Time-lapse imaging of Myo2-GFP (green) and membranes labeled with FM4-64 (red) following treatment with MeOH. See also Supplemental Video 4.","Time-lapse of Z-stacks of optical sections from a representative hypha during actomyosin ring constriction, showing the localization of Lifeact-GFP (green), FM4-64 (red), and calcofluor (blue) to label actin, cell membrane and cell wall, respectively. See also Supplemental Video 3."],"repository":["bioimages"],"figure_sub":["Image 30437 (Figure 3 - A)","Image 30436 (Figure 2 - E)","Figure 8 - A","Image 131134 (Figure 7 - C)","Image 30439 (Figure 3 - B)","Figure 7 - A","Figure 7 - C","Image 30442 (Figure 7 - C)","Figure 3 - A","Figure 3 - B","Image 30441 (Figure 3 - C)","Figure 3 - C","Image 30438 (Figure 3 - B)","Image 131129 (Figure 7 - A)","Image 30440 (Figure 3 - C)","Image 131131 (Figure 7 - A)","Image 131128 (Figure 7 - A)","Image 131109 (Figure 2 - D)","Image 30445 (Figure 8 - A)","Figure 2 - E","Figure 7","Figure 8","Image 131133 (Figure 8 - A)","Figure 2 - D","Figure 3","Figure 2"],"pubmed_authors":["Fong Yew Leong","Mohan K. Balasubramanian","Yinxiao Chen","Meredith E.K. Calvert","Gregory Jedd","Keng-Hwee Chiam","Graham D. Wright"],"additional_accession":[]},"is_claimable":false,"name":"Myosin concentration underlies cell size–dependent scalability of actomyosin ring constriction","description":null,"dates":{"release":"2011-11-28T11:21:59Z","modification":"2018-11-29T11:21:59Z","creation":"2018-11-29T11:21:59Z"},"accession":"S-JCBD-201101055","cross_references":{"doi":["10.1083/jcb.201101055"]}}