bioimagesUnknownMeredith E.K. CalvertThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201101055JCB

A Z-stack of optical sections from a representative hypha during actomyosin ring constriction, showing the localization of Lifeact-GFP (green), FM4-64 (red), and calcofluor (blue) to label actin, cell membrane and cell wall, respectively. See also Supplemental Video 3.

Time-lapse imaging shows the decrease in ring diameter over time within a representative hypha expressing Lifeact-GFP (green) and membranes labeled with FM4-64 (red). <br />

A Z-stack of optical sections showing adjacent actomyosin rings immediately following the photobleaching of Myo-2-GFP (green). The ring on the right has been bleached. See also Supplemental Video 4.

Time-lapse imaging shows that the actin binding probe Lifeact-GFP accumulated along the hyphal cortex, assembled into a contractile ring, and persisted at the ring throughout septation. See also Supplemental video 2.

Time-lapse imaging shows the decrease in ring diameter over time within a representative hypha expressing Myo2-GFP (green) and membranes labeled with FM4-64 (red). See also Supplemental Video 1.

Time-lapse imaging shows the decrease in ring diameter over time within a representative hypha expressing Lifeact-GFP (green) and membranes labeled with FM4-64 (red).

Single image showing Lifeact-GFP (green) and membranes labeled with FM4-64 (red) following inhibition of actin polymerization with 10 um Latrunculin A.

Delocalization of Myo-2-GFP (green) from the ring and stalled membrane closure (FM4-64, red) following treatement with 1mM blebbistatin

Time-lapse of Z-stacks of optical sections showing adjacent actomyosin rings during fluorescence recovery after photobleaching (FRAP) of Myo2-GFP (green). The ring on the right has been bleached. See also Supplemental Video 4.

Time-lapse imaging shows Lifeact-GFP (green) and membranes labeled with FM4-64 (red) following treatment with DMSO.

Time-lapse imaging shows the decrease in ring diameter over time within a representative hypha expressing Myo2-GFP (green) and membranes labeled with FM4-64 (red).

A Z-stack of optical sections showing adjacent actomyosin rings prior to the photobleaching of Myo-2-GFP (green). See also Supplemental Video 4.<br />

Time-lapse imaging of Myo2-GFP (green) and membranes labeled with FM4-64 (red) following treatment with MeOH. See also Supplemental Video 4.

Time-lapse of Z-stacks of optical sections from a representative hypha during actomyosin ring constriction, showing the localization of Lifeact-GFP (green), FM4-64 (red), and calcofluor (blue) to label actin, cell membrane and cell wall, respectively. See also Supplemental Video 3.

bioimagesImage 30437 (Figure 3 - A)Image 30436 (Figure 2 - E)Figure 8 - AImage 131134 (Figure 7 - C)Image 30439 (Figure 3 - B)Figure 7 - AFigure 7 - CImage 30442 (Figure 7 - C)Figure 3 - AFigure 3 - BImage 30441 (Figure 3 - C)Figure 3 - CImage 30438 (Figure 3 - B)Image 131129 (Figure 7 - A)Image 30440 (Figure 3 - C)Image 131131 (Figure 7 - A)Image 131128 (Figure 7 - A)Image 131109 (Figure 2 - D)Image 30445 (Figure 8 - A)Figure 2 - EFigure 7Figure 8Image 131133 (Figure 8 - A)Figure 2 - DFigure 3Figure 2Fong Yew LeongMohan K. BalasubramanianYinxiao ChenMeredith E.K. CalvertGregory JeddKeng-Hwee ChiamGraham D. WrightfalseMyosin concentration underlies cell size–dependent scalability of actomyosin ring constriction2011-11-28T11:21:59Z2018-11-29T11:21:59Z2018-11-29T11:21:59ZS-JCBD-20110105510.1083/jcb.201101055