bioimagesUnknownAnna Krzywicka-RackaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201101073JCB

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A binuclear cell (white frame) exhibits multiple rounds of cleavage failure resulting in a large multinucleated cell. Shown is a single frame (29:45) from time-lapse video microscopy.

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A binuclear cell (white frame) exhibits multiple rounds of cleavage failure resulting in a large multinucleated cell. Shown is a single frame (37:00) from time-lapse video microscopy.

RPE-1 cells expressing centrin-GFP and labeled for DNA with DAPI (blue).

Daughters of a binucleate cell fixed 15 hours after first tetraploid mitosis. Cells are labeled for p21 (green).

Labeling for p21 (green) in binucleate cells that arrested after cleavage failure.

RPE-1 cells expressing centrin-GFP and immunostained with gamma-tubulin antibody (red).

Daughters of a binucleate cell fixed 15 hours after first tetraploid mitosis. Cells are labeled for p53 (red).

Labeling for p53 (red) in binucleate cells that arrested after cleavage failure.

Labeling for DAPI (blue) in binucleate cells that arrested after cleavage failure.

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A binuclear cell (white frame) exhibits multiple rounds of cleavage failure resulting in a large multinucleated cell. Shown is a single frame (69:30) from time-lapse video microscopy.

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A multinucleate cell (white frame) undergoes cell death following multiple rounds of cleavage failure. Shown is a single frame (10:36) from time-lapse video microscopy.

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A binuclear cell (white frame) exhibits multiple rounds of cleavage failure resulting in a large multinucleated cell. Shown is a single frame (45:30) from time-lapse video microscopy.

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A binuclear cell (white frame) exhibits multiple rounds of cleavage failure resulting in a large multinucleated cell. Shown is a single frame (78:24) from time-lapse video microscopy.

Live cell imaging for centrin-GFP of centriole duplication and distribution thirteen hours after cytochalasin D washout.

Daughters of a binucleate cell fixed 15 hours after first tetraploid mitosis. Cells are labeled for DAPI (blue).

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A binuclear cell (white frame) exhibits multiple rounds of cleavage failure resulting in a large multinucleated cell. Shown is a single frame (13:15) from time-lapse video microscopy.

Live cell imaging for centrin-GFP of centriole duplication and distribution two hours after cytochalasin D washout.

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A binuclear cell (white frame) exhibits multiple rounds of cleavage failure resulting in a large multinucleated cell. Shown is a single frame (3:00) from time-lapse video microscopy.

Phase contrast imaging of centriole duplication and distribution in cells expressing centrin-GFP thirteen hours after cytochalasin D washout.

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A binuclear cell (white frame) exhibits multiple rounds of cleavage failure resulting in a large multinucleated cell. Shown is a single frame (21:24) from time-lapse video microscopy.

Live cell imaging for centrin-GFP of centriole duplication and distribution nineteen hours after cytochalasin D washout.

Fluorescense imaging of centrin1-GFP of centriole duplication and distribution in cells expressing centrin-GFP nineteen hours after cytochalasin D washout. Shown is asymmetric centriole distribution to the daughters of a binucleate cell after biploar mitosis.

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A multinucleate cell (white frame) undergoes cell death following multiple rounds of cleavage failure. Shown is a single frame (0:00) from time-lapse video microscopy.

Phase contrast imaging of centriole duplication and distribution in cells expressing centrin-GFP nineteen hours after cytochalasin D washout. Shown is asymmetric centriole distribution to the daughters of a binucleate cell after biploar mitosis.

Daughters of a binucleate cell fixed 15 hours after first tetraploid mitosis. Cells are labeled for p53 (red), p21 (green), and DAPI (blue).

Labeling for p53 (red), p21 (green), and DAPI (blue) in binucleate cells that arrested after cleavage failure.

Phase contrast microscopy of HCT116 p53-/- cells after cleavage failure. A binuclear cell (white frame) exhibits multiple rounds of cleavage failure resulting in a large multinucleated cell. Shown is a single frame (102:18) from time-lapse video microscopy.

Phase contrast imaging of centriole duplication and distribution in cells expressing centrin-GFP two hours after cytochalasin D washout.

RPE-1 cells expressing centrin1-GFP(green).

Phase contrast imaging of centriole duplication and distribution in cells expressing centrin-GFP nineteen hours after cytochalasin D washout.

bioimagesFigure 3 - C.1 - multiple cleavage failureImage 33273 (Supplemental Figure 1 - B.3 - 19 h after cytochalasin D wash out)Figure 2 - A - binucleate cell following cleavage failureImage 33145 (Figure 2 - A - binucleate cell following cleavage failure)Supplemental Figure 1 - B.4 - 19 h after cytochalasin D wash out, asymmetric centriole distributionSupplemental Figure 1Image 33144 (Figure 2 - A - binucleate cell following cleavage failure)Image 33306 (Figure 3 - C.1 - multiple cleavage failure)Image 33246 (Supplemental Figure 1 - B.2 - 13 h after cytochalasin D wash out)Supplemental Figure 1 - B.3 - 19 h after cytochalasin D wash outImage 33310 (Figure 3 - C.2 - multinucleated cell death)Figure 2 - A - daughter cells after tetraploid mitosisImage 33274 (Supplemental Figure 1 - B.3 - 19 h after cytochalasin D wash out)Image 33190 (Supplemental Figure 1 - B.1 - 2 h after cytochalasin D wash out)Image 33191 (Supplemental Figure 1 - B.1 - 2 h after cytochalasin D wash out)Image 33143 (Figure 2 - A - binucleate cell following cleavage failure)Image 32962 (Figure 2 - A - daughter cells after tetraploid mitosis)Image 33186 (Supplemental Figure 1 - B.1 - 2 h after cytochalasin D wash out)Image 33303 (Figure 3 - C.1 - multiple cleavage failure)Supplemental Figure 1 - B.1 - 2 h after cytochalasin D wash outImage 33288 (Supplemental Figure 1 - A)Image 33245 (Supplemental Figure 1 - B.2 - 13 h after cytochalasin D wash out)Supplemental Figure 1 - AImage 33267 (Supplemental Figure 1 - B.3 - 19 h after cytochalasin D wash out)Image 33276 (Supplemental Figure 1 - B.3 - 19 h after cytochalasin D wash out)Image 33299 (Figure 3 - C.1 - multiple cleavage failure)Image 33157 (Supplemental Figure 1 - B.1 - 2 h after cytochalasin D wash out)Image 33304 (Figure 3 - C.1 - multiple cleavage failure)Image 33298 (Figure 3 - C.1 - multiple cleavage failure)Figure 3 - C.2 - multinucleated cell deathImage 32963 (Figure 2 - A - daughter cells after tetraploid mitosis)Image 33292 (Supplemental Figure 1 - A)Image 33277 (Supplemental Figure 1 - B.3 - 19 h after cytochalasin D wash out)Image 33317 (Supplemental Figure 1 - B.4 - 19 h after cytochalasin D wash out, asymmetric centriole distribution)Image 33309 (Figure 3 - C.2 - multinucleated cell death)Image 33295 (Figure 3 - C.1 - multiple cleavage failure)Image 33289 (Supplemental Figure 1 - A)Image 33242 (Supplemental Figure 1 - B.2 - 13 h after cytochalasin D wash out)Supplemental Figure 1 - B.2 - 13 h after cytochalasin D wash outImage 33142 (Figure 2 - A - binucleate cell following cleavage failure)Image 33275 (Supplemental Figure 1 - B.3 - 19 h after cytochalasin D wash out)Image 33305 (Figure 3 - C.1 - multiple cleavage failure)Image 33318 (Supplemental Figure 1 - B.4 - 19 h after cytochalasin D wash out, asymmetric centriole distribution)Image 33241 (Supplemental Figure 1 - B.2 - 13 h after cytochalasin D wash out)Image 33297 (Figure 3 - C.1 - multiple cleavage failure)Image 33266 (Supplemental Figure 1 - B.3 - 19 h after cytochalasin D wash out)Image 33272 (Supplemental Figure 1 - B.3 - 19 h after cytochalasin D wash out)Image 32964 (Figure 2 - A - daughter cells after tetraploid mitosis)Image 32965 (Figure 2 - A - daughter cells after tetraploid mitosis)Image 33296 (Figure 3 - C.1 - multiple cleavage failure)Figure 3Figure 2Anna Krzywicka-RackaGreenfield SluderfalseRepeated cleavage failure does not establish centrosome amplification in untransformed human cells2011-07-25T11:22:00Z2018-11-29T11:22:00Z2018-11-29T11:22:00ZS-JCBD-20110107310.1083/jcb.201101073