{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Steph D. Anastasia"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-201108108"],"attach_to":["JCB"],"legend":["The pkc1-14 mutant compromises functions of Pkc1 that are important when growth is slowed due to nutrient limitation, and the effects of the pkc1-14 mutant can be rescued by loss of Cdk1 inhibitory phosphorylation in a swe1Δ mutant background. The control (wild-type) yeast cells shown here exhibit no visible bud elongation. These cells were inoculated into YPD media at low density and grown at 30°C until they reached an optical density of approximately 1.7. Cells were fixed with formaldehyde and photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera. Images were acquired using Zeiss Axiovision software.","The response to arrest of membrane traffic is not a consequence of indirect effects on actin. These wild-type yeast cells were grown to log phase in YPD media and then shifted to the restrictive temperature (34°C) for 5 minutes. Cells were then fixed and stained with FITC-phalloidin. Cells were photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera.  Images were acquired using Zeiss Axiovision software.  <br /><br />","The pkc1-14 mutant compromises functions of Pkc1 that are important when growth is slowed due to nutrient limitation, and the effects of the pkc1-14 mutant can be rescued by loss of Cdk1 inhibitory phosphorylation in a swe1Δ mutant background. The pkc1-14 swe1∆ yeast cells shown here do not exhibit the dramatic bud elongation phenotype seen in pkc1-14 cells. These cells were inoculated into YPD media at low density and grown at 30°C until they reached an optical density of approximately 1.7. Cells were fixed with formaldehyde and photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera. Images were acquired using Zeiss Axiovision software.","The response to arrest of membrane traffic is not a consequence of indirect<br />effects on actin. These sec6-4 yeast cells were grown to log phase in YPD media and then shifted to the restrictive temperature (34°C) for 5 minutes. Cells were then fixed and stained with FITC-phalloidin. Cells were photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera.  Images were acquired using Zeiss Axiovision software.","The pkc1-14 mutant compromises functions of Pkc1 that are important when growth is slowed due to nutrient limitation, and the effects of the pkc1-14 mutant can be rescued by loss of Cdk1 inhibitory phosphorylation in a swe1Δ mutant background. The pkc1-14 yeast cells shown here exhibit a dramatic bud elongation phenotype. These cells were inoculated into YPD media at low density and grown at 30°C until they reached an optical density of approximately 1.7. Cells were fixed with formaldehyde and photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera. Images were acquired using Zeiss Axiovision software."],"repository":["bioimages"],"figure_sub":["Figure 4","Figure 4 - B","Image 133653 (Figure 6 - B)","Image 133656 (Figure 4 - B)","Figure 6","Image 133655 (Figure 4 - B)","Figure 6 - B","Image 133654 (Figure 6 - B)","Image 133652 (Figure 6 - B)"],"pubmed_authors":["Steph D. Anastasia","Tracy MacDonough","Vu Thai","Duy Linh Nguyen","Melissa Meloy","Douglas R. Kellogg"],"additional_accession":[]},"is_claimable":false,"name":"A link between mitotic entry and membrane growth suggests a novel model for cell size control","description":null,"dates":{"release":"2012-03-26T11:22:25Z","modification":"2018-11-29T11:22:25Z","creation":"2018-11-29T11:22:25Z"},"accession":"S-JCBD-201108108","cross_references":{"doi":["10.1083/jcb.201108108"]}}