bioimagesUnknownSteph D. AnastasiaThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-201108108JCB

The pkc1-14 mutant compromises functions of Pkc1 that are important when growth is slowed due to nutrient limitation, and the effects of the pkc1-14 mutant can be rescued by loss of Cdk1 inhibitory phosphorylation in a swe1Δ mutant background. The control (wild-type) yeast cells shown here exhibit no visible bud elongation. These cells were inoculated into YPD media at low density and grown at 30°C until they reached an optical density of approximately 1.7. Cells were fixed with formaldehyde and photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera. Images were acquired using Zeiss Axiovision software.

The response to arrest of membrane traffic is not a consequence of indirect effects on actin. These wild-type yeast cells were grown to log phase in YPD media and then shifted to the restrictive temperature (34°C) for 5 minutes. Cells were then fixed and stained with FITC-phalloidin. Cells were photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera. Images were acquired using Zeiss Axiovision software. <br /><br />

The pkc1-14 mutant compromises functions of Pkc1 that are important when growth is slowed due to nutrient limitation, and the effects of the pkc1-14 mutant can be rescued by loss of Cdk1 inhibitory phosphorylation in a swe1Δ mutant background. The pkc1-14 swe1∆ yeast cells shown here do not exhibit the dramatic bud elongation phenotype seen in pkc1-14 cells. These cells were inoculated into YPD media at low density and grown at 30°C until they reached an optical density of approximately 1.7. Cells were fixed with formaldehyde and photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera. Images were acquired using Zeiss Axiovision software.

The response to arrest of membrane traffic is not a consequence of indirect<br />effects on actin. These sec6-4 yeast cells were grown to log phase in YPD media and then shifted to the restrictive temperature (34°C) for 5 minutes. Cells were then fixed and stained with FITC-phalloidin. Cells were photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera. Images were acquired using Zeiss Axiovision software.

The pkc1-14 mutant compromises functions of Pkc1 that are important when growth is slowed due to nutrient limitation, and the effects of the pkc1-14 mutant can be rescued by loss of Cdk1 inhibitory phosphorylation in a swe1Δ mutant background. The pkc1-14 yeast cells shown here exhibit a dramatic bud elongation phenotype. These cells were inoculated into YPD media at low density and grown at 30°C until they reached an optical density of approximately 1.7. Cells were fixed with formaldehyde and photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera. Images were acquired using Zeiss Axiovision software.

bioimagesFigure 4Figure 4 - BImage 133653 (Figure 6 - B)Image 133656 (Figure 4 - B)Figure 6Image 133655 (Figure 4 - B)Figure 6 - BImage 133654 (Figure 6 - B)Image 133652 (Figure 6 - B)Steph D. AnastasiaTracy MacDonoughVu ThaiDuy Linh NguyenMelissa MeloyDouglas R. KelloggfalseA link between mitotic entry and membrane growth suggests a novel model for cell size control2012-03-26T11:22:25Z2018-11-29T11:22:25Z2018-11-29T11:22:25ZS-JCBD-20110810810.1083/jcb.201108108